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| Product Name
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Gastric Inhibitory Polypeptide (GIP), ELISA Kit ★Popular Item★
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| Also Known As
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Chicken Gastric Inhibitory Polypeptide (GIP) ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| OMIM
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137240
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| Species Reactivity
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Chicken
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| Specificity
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No cross-reaction with other factors.
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| Samples
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Chicken serum, plasma or cell culture supernatant and organizations in the natural and recombinant GIP concentration.
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| Assay Type
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Sandwich
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| Detection Range
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5000 pg/ml-78 pg/ml
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| Sensitivity
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20 pg/ml.
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| Intra-assay Precision
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<= 8%
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| Inter-assay Precision
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<= 12%
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| Preparation and Storage
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Store all reagents at 2-8 degree C.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of GIP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for GIP purchase
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MBS261884 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gastric Inhibitory Polypeptide (GIP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5000 pg/ml-78 pg/ml in biological research samples containing GIP, with an estimated sensitivity of 20 pg/ml. The ELISA analytical biochemical technique of the MBS261884 kit is based on GIP antibody-GIP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GIP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GIP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Chicken serum, plasma or cell culture supernatant and organizations in the natural and recombinant GIP concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GIP elisa kit
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Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken GIP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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| Typical Testing Data/Standard Curve of GIP elisa kit
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| Sample Manual Insert of MBS261884. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for GIP. It may not necessarily be applicable to this product. |
| NCBI GI #
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4758436
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| NCBI GeneID
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2695
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| NCBI Accession #
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NP_004114.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_004123.2
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[Other Products]
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| UniProt Secondary Accession #
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Q4VB42; Q6NTD3
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[Other Products]
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| UniProt Related Accession #
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P09681
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[Other Products]
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| Molecular Weight
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17,108 Da
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| NCBI Official Full Name
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gastric inhibitory polypeptide preproprotein
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| NCBI Official Synonym Full Names
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gastric inhibitory polypeptide
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| NCBI Official Symbol
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GIP
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[Similar Products]
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| NCBI Protein Information
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gastric inhibitory polypeptide; incretin hormone; glucose-dependent insulinotropic polypeptide
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| UniProt Protein Name
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Gastric inhibitory polypeptide
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| UniProt Synonym Protein Names
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Glucose-dependent insulinotropic polypeptide; Incretin hormone
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| Protein Family
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Gastric inhibitory polypeptide
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| UniProt Gene Name
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GIP
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[Similar Products]
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| UniProt Synonym Gene Names
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GIP
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[Similar Products]
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| UniProt Entry Name
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GIP_HUMAN
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| NCBI Summary for GIP
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This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]
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| UniProt Comments for GIP
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GIP: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Belongs to the glucagon family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 17q21.3-q22
Cellular Component: extracellular space; cell soma; endoplasmic reticulum lumen; extracellular region
Molecular Function: hormone activity
Biological Process: response to drug; response to peptide hormone stimulus; adult locomotory behavior; positive regulation of insulin secretion; female pregnancy; sensory perception of pain; signal transduction; response to lipid; response to amino acid stimulus; response to organic cyclic substance; endocrine pancreas development; memory; response to starvation; response to selenium ion; cellular protein metabolic process; response to glucose stimulus; response to axon injury; regulation of insulin secretion
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| Research Articles on GIP
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1. Data from studies in healthy Japanese men suggest that plasma GIP levels in postprandial period are dose dependently increased by fat content of meals of ordinary size, despite the amount of additional fat being relatively small.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with GIP elisa kit | Pathways associated with GIP elisa kit |
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| Diseases associated with GIP elisa kit | Organs/Tissues associated with GIP elisa kit |
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ELISA Kit 
