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HSPA1A elisa kit :: Chicken Heat Shock 70kDa Protein 1A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS740753
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Heat Shock 70kDa Protein 1A (HSPA1A), ELISA Kit

 Also Known As   

Chicken Heat Shock 70kDa Protein 1A ELISA Kit

 Product Gene Name   

HSPA1A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 6; NC_000006.11 (31783291..31785719). Location: 6p21.3
 OMIM    140550
 3D Structure    ModBase 3D Structure for P08107
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 Species Reactivity    Chicken
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 Specificity    This assay has high sensitivity and excellent specificity for detection of HSP-701A. No significant cross-reactivity or interference between HSP-701A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSP-701A and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HSPA1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HSPA1A purchase    MBS740753 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Heat Shock 70kDa Protein 1A (HSPA1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSPA1A, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS740753 kit is based on HSPA1A antibody-HSPA1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSPA1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSPA1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for HSPA1A elisa kit

   Intended Uses: This HSP-701A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken HSP-701A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: HSP-701A ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HSP-701A. Standards or samples are then added to the microtiter plate wells and HSP-701A if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HSP-701A present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HSP-701A are added to each well to "sandwich" the HSP-701A immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HSP-701A and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP-701A concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for HSPA1A elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of HSPA1A elisa kit    HSPA1A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS740753. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HSPA1A. It may not necessarily be applicable to this product.
 NCBI GI #    194248072
 NCBI GeneID    3303
 NCBI Accession #    NP_005336.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_005345.5 [Other Products]
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 UniProt Primary Accession #    P08107 [Other Products]
 UniProt Secondary Accession #    P19790; Q5JQI4; Q5SP17; Q9UQL9; Q9UQM0 [Other Products]
 UniProt Related Accession #    P08107; Q59EJ3; Q9UQC1 [Other Products]
 Molecular Weight    70,052 Da
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 NCBI Official Full Name    heat shock 70 kDa protein 1A/1B
 NCBI Official Synonym Full Names    heat shock 70kDa protein 1A
 NCBI Official Symbol    HSPA1A [Similar Products]
 NCBI Official Synonym Symbols   
HSP72; HSPA1; HSP70I; HSPA1B; HSP70-1; HSP70-1A
[Similar Products]
 NCBI Protein Information    heat shock 70 kDa protein 1A/1B; HSP70-1/HSP70-2; heat shock 70kD protein 1A; heat shock-induced protein; heat shock 70 kDa protein 1/2; dnaK-type molecular chaperone HSP70-1
 UniProt Protein Name    Heat shock 70 kDa protein 1A/1B
 UniProt Synonym Protein Names   
Heat shock 70 kDa protein 1/2
 Protein Family    Heat shock 70 kDa protein
 UniProt Gene Name    HSPA1A [Similar Products]
 UniProt Synonym Gene Names    HSPA1; HSP70-1/HSP70-2 [Similar Products]
 UniProt Entry Name    HSP71_HUMAN
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 NCBI Summary for HSPA1A    This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HSPA1A    HSP70: a critical chaperone protein that has a high affinity for unfolded polypeptide chains. It binds extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In cooperation with other chaperones, hsp70 stabilizes preexistent proteins against aggregation and mediates the folding of newly translated polypeptides in the cytosol as well as within organelles. Mitochondrial HSP70 is crucial to the import process: mutant forms of HSP70 fail to import precursor proteins. Is anti-apoptotic in sympathetic neurones and mediates this effect primarily by suppressing c-Jun transcriptional signalling. Interacts with tau protein and mediates proper folding of tau. Can promote the degradation of tau protein. Triptolide, a potential therapeutic agent for progression/metastasis of pancreatic cancer, causes pancreatic cancer cell death by induction of apoptosis, an effect mediated by the inhibition of HSP70.

Protein type: Chaperone; Heat shock protein; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: signalosome; focal adhesion; mitochondrion; endoplasmic reticulum; inclusion body; ribonucleoprotein complex; cytosol; centriole; nucleoplasm; perinuclear region of cytoplasm; cytoplasm; nuclear speck; nucleus; vesicle; ubiquitin ligase complex

Molecular Function: viral receptor activity; protein binding; enzyme binding; G-protein-coupled receptor binding; ubiquitin protein ligase binding; heat shock protein binding; ATPase activity; double-stranded RNA binding; unfolded protein binding; protein N-terminus binding; ATPase activity, coupled; ATP binding

Biological Process: negative regulation of cell proliferation; entry of virus into host cell; protein stabilization; positive regulation of erythrocyte differentiation; mRNA catabolic process; gene expression; protein refolding; negative regulation of protein ubiquitination; negative regulation of cell growth; response to unfolded protein; negative regulation of apoptosis
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 Research Articles on HSPA1A    1. HSPA1A, in combination with HDACi, may have an effect on lymphoid neoplasms
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HSPA1A elisa kitPathways associated with HSPA1A elisa kit
 Reference Product  PubMed Publications
 DNAJB1 elisa kit  >11 publications with HSPA1A and DNAJB1
 HSPA8 elisa kit  >9 publications with HSPA1A and HSPA8
 HSF1 elisa kit  >7 publications with HSPA1A and HSF1
 BCL2 elisa kit  >6 publications with HSPA1A and BCL2
 STIP1 elisa kit  >3 publications with HSPA1A and STIP1
 NQO1 elisa kit  >2 publications with HSPA1A and NQO1
 BAG3 elisa kit  >2 publications with HSPA1A and BAG3
 APAF1 elisa kit  >2 publications with HSPA1A and APAF1
 SOX9 elisa kit  >1 publications with HSPA1A and SOX9
 PTGES3 elisa kit  >1 publications with HSPA1A and PTGES3
 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Apoptosis Modulation By HSP70 Pathway antibodies  Apoptosis Modulation By HSP70 Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 FOXM1 Transcription Factor Network Pathway antibodies  FOXM1 Transcription Factor Network Pathway Diagram
Diseases associated with HSPA1A elisa kitOrgans/Tissues associated with HSPA1A elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >27 publications with HSPA1A and Neoplasms
 Inflammation Antibodies  >15 publications with HSPA1A and Inflammation
 Drug Toxicity Antibodies  >13 publications with HSPA1A and Drug Toxicity
 Cardiovascular Diseases Antibodies  >12 publications with HSPA1A and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >8 publications with HSPA1A and Nervous System Diseases
 Carcinoma Antibodies  >6 publications with HSPA1A and Carcinoma
 Necrosis Antibodies  >6 publications with HSPA1A and Necrosis
 Heart Diseases Antibodies  >6 publications with HSPA1A and Heart Diseases
 Lung Diseases Antibodies  >6 publications with HSPA1A and Lung Diseases
 Atherosclerosis Antibodies  >5 publications with HSPA1A and Atherosclerosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >25 publications with HSPA1A and Blood
 Liver Antibodies  >14 publications with HSPA1A and Liver
 Brain Antibodies  >10 publications with HSPA1A and Brain
 Vascular Antibodies  >9 publications with HSPA1A and Vascular
 Nerve Antibodies  >7 publications with HSPA1A and Nerve
 Muscle Antibodies  >7 publications with HSPA1A and Muscle
 Kidney Antibodies  >7 publications with HSPA1A and Kidney
 Skin Antibodies  >7 publications with HSPA1A and Skin
 Lung Antibodies  >6 publications with HSPA1A and Lung
 Heart Antibodies  >6 publications with HSPA1A and Heart
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