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IAA elisa kit :: Chicken Insulin Autoantibody (IAA) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS265123
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Insulin Autoantibody (IAA), ELISA Kit

★Popular Item★
 Also Known As   

Chicken Insulin Autoantibody (IAA) ELISA Kit

 Product Gene Name   

IAA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Chicken
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 Specificity    No cross-reaction with other factors.
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 Samples    Chicken serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IAA concentration
 Assay Type    Sandwich
 Detection Range    200 ng/ml-3.12 ng/ml
 Sensitivity    the minimum detectable Chicken IAA up to 0.6 ng/ml.
 Intra-assay Precision    ? 8%
 Inter-assay Precision    ? 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IAA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IAA purchase    MBS265123 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Insulin Autoantibody (IAA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 200 ng/ml-3.12 ng/ml in biological research samples containing IAA, with an estimated sensitivity of the minimum detectable Chicken IAA up to 0.6 ng/ml. The ELISA analytical biochemical technique of the MBS265123 kit is based on IAA antibody-IAA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IAA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IAA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Chicken serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IAA concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IAA elisa kit

   Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken IAA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of IAA elisa kit    IAA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS265123. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IAA. It may not necessarily be applicable to this product.
 NCBI GI #    109148522
 NCBI GeneID    723961
 NCBI Accession #    NP_001035835.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001042376.2 [Other Products]
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 UniProt Secondary Accession #    Q1WM24 [Other Products]
 UniProt Related Accession #    F8WCM5 [Other Products]
 Molecular Weight    11,981 Da
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 NCBI Official Full Name    insulin, isoform 2
 NCBI Official Synonym Full Names    INS-IGF2 readthrough
 NCBI Official Symbol    INS-IGF2 [Similar Products]
 NCBI Official Synonym Symbols   
INSIGF
[Similar Products]
 NCBI Protein Information    insulin, isoform 2; INS-IGF2 readthrough transcript protein; insulin- insulin-like growth factor 2 read-through product
 UniProt Protein Name    Insulin, isoform 2
 UniProt Synonym Protein Names   
INS-IGF2 readthrough transcript protein
 UniProt Gene Name    INS-IGF2 [Similar Products]
 UniProt Entry Name    INSR2_HUMAN
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 NCBI Summary for IAA    This locus includes two alternatively spliced read-through transcript variants which align to the INS gene in the 5' region and to the IGF2 gene in the 3' region. One transcript is predicted to encode a protein which shares the N-terminus with the INS protein but has a distinct and longer C-terminus, whereas the other transcript is a candidate for nonsense-mediated decay (NMD). The transcripts are imprinted and are paternally expressed in the limb and eye. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IAA    INS-IGF2: 2 isoforms of the human protein are produced by alternative splicing

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular region

Molecular Function: hormone activity
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 Research Articles on IAA    1. INS-IGF2 and insulin may share autoantibody-binding sites, thus complicating the notion that insulin is the primary autoantigen in type 1 diabetes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IAA elisa kit
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 IRS Activation Pathway antibodies  IRS Activation Pathway Diagram
 IRS-related Events Pathway antibodies  IRS-related Events Pathway Diagram
 Insulin Processing Pathway antibodies  Insulin Processing Pathway Diagram
 Insulin Receptor Recycling Pathway antibodies  Insulin Receptor Recycling Pathway Diagram
 Insulin Receptor Signalling Cascade Pathway antibodies  Insulin Receptor Signalling Cascade Pathway Diagram
 Integration Of Energy Metabolism Pathway antibodies  Integration Of Energy Metabolism Pathway Diagram
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