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IL-1 elisa kit :: Chicken Interleukin 1 (IL-1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS261118
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 1 (IL-1), ELISA Kit

★Popular Item★
 Also Known As   

Chicken Interleukin 1 (IL-1) ELISA Kit

 Product Gene Name   

IL-1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    147811
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 Species Reactivity    Chicken
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 Specificity    No cross-reaction with other factors.
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 Samples    Chicken serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IL-1 concentration
 Assay Type    Sandwich
 Detection Range    1000 pg/ml-15.6 pg/ml
 Sensitivity    the minimum detectable Chicken IL-1 up to 5 pg/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-1 purchase    MBS261118 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 1 (IL-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1000 pg/ml-15.6 pg/ml in biological research samples containing IL-1, with an estimated sensitivity of the minimum detectable Chicken IL-1 up to 5 pg/ml. The ELISA analytical biochemical technique of the MBS261118 kit is based on IL-1 antibody-IL-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Chicken serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IL-1 concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-1 elisa kit

  
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken IL-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of IL-1 elisa kit    IL-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS261118. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-1. It may not necessarily be applicable to this product.
 NCBI GI #    387527984
 NCBI GeneID    7850
 NCBI Accession #    NP_001248348.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001261419.1 [Other Products]
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 UniProt Secondary Accession #    Q6LCE6; Q9UE68; D3DVJ5 [Other Products]
 UniProt Related Accession #    P27930 [Other Products]
 Molecular Weight    33,622 Da
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 NCBI Official Full Name    interleukin-1 receptor type 2 isoform 2
 NCBI Official Synonym Full Names    interleukin 1 receptor, type II
 NCBI Official Symbol    IL1R2 [Similar Products]
 NCBI Official Synonym Symbols   
IL1RB; CD121b; IL1R2c; CDw121b; IL-1R-2; IL-1RT2; IL-1RT-2
[Similar Products]
 NCBI Protein Information    interleukin-1 receptor type 2; IL-1R-beta; antigen CDw121b; IL-1 type II receptor; interleukin-1 receptor beta; interleukin-1 receptor type II; CD121 antigen-like family member B; type II interleukin-1 receptor, beta; interleukin 1 receptor type II variant 3
 UniProt Protein Name    Interleukin-1 receptor type 2
 UniProt Synonym Protein Names   
CD121 antigen-like family member B; CDw121b; IL-1 type II receptor; Interleukin-1 receptor beta; IL-1R-beta; Interleukin-1 receptor type II; CD_antigen: CD121bCleaved into the following 2 chains:Interleukin-1 receptor type 2, membrane form; mIL-1R2; mIL-1RII; Interleukin-1 receptor type 2, soluble form; sIL-1R2; sIL-1RII
 UniProt Gene Name    IL1R2 [Similar Products]
 UniProt Synonym Gene Names    IL1RB; IL-1R-2; IL-1RT-2; IL-1RT2; IL-1R-beta; mIL-1R2; mIL-1RII; sIL-1R2; sIL-1RII [Similar Products]
 UniProt Entry Name    IL1R2_HUMAN
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 NCBI Summary for IL-1    The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Alternative splicing results in multiple transcript variants and protein isoforms. Alternative splicing produces both membrane-bound and soluble proteins. A soluble protein is also produced by proteolytic cleavage. [provided by RefSeq, May 2012]
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 UniProt Comments for IL-1    IL-1RB: Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Belongs to the interleukin-1 receptor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: plasma membrane; integral to membrane; extracellular region

Molecular Function: protein binding; interleukin-1, Type II, blocking receptor activity; interleukin-1 receptor activity

Biological Process: cytokine and chemokine mediated signaling pathway; immune response
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 Research Articles on IL-1    1. Data indicate a significant increase in the levels of IL-8, TNF-alpha and IL-1R2 in the CSF of both meningitis groups as compared to controls, and the concentrations of IFN-gamma and IL-1 differed significantly only between the mumps group and control.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-1 elisa kitPathways associated with IL-1 elisa kit
 Reference Product  PubMed Publications
 IL1R1 elisa kit  >16 publications with IL-1 and IL1R1
 IL1B elisa kit  >12 publications with IL-1 and IL1B
 IL1RN elisa kit  >5 publications with IL-1 and IL1RN
 IL1A elisa kit  >3 publications with IL-1 and IL1A
 IL1RAP elisa kit  >3 publications with IL-1 and IL1RAP
 NFKB1 elisa kit  >2 publications with IL-1 and NFKB1
 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
Diseases associated with IL-1 elisa kitOrgans/Tissues associated with IL-1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >24 publications with IL-1 and Inflammation
 Necrosis Antibodies  >11 publications with IL-1 and Necrosis
 Lung Diseases Antibodies  >10 publications with IL-1 and Lung Diseases
 Cardiovascular Diseases Antibodies  >8 publications with IL-1 and Cardiovascular Diseases
 Spondylitis, Ankylosing Antibodies  >6 publications with IL-1 and Spondylitis, Ankylosing
 Hypersensitivity Antibodies  >5 publications with IL-1 and Hypersensitivity
 Colitis, Ulcerative Antibodies  >4 publications with IL-1 and Colitis, Ulcerative
 Heart Diseases Antibodies  >4 publications with IL-1 and Heart Diseases
 Lung Neoplasms Antibodies  >3 publications with IL-1 and Lung Neoplasms
 Pain Antibodies  >3 publications with IL-1 and Pain
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >35 publications with IL-1 and Blood
 Lung Antibodies  >15 publications with IL-1 and Lung
 Uterus Antibodies  >12 publications with IL-1 and Uterus
 Prostate Antibodies  >5 publications with IL-1 and Prostate
 Brain Antibodies  >4 publications with IL-1 and Brain
 Skin Antibodies  >4 publications with IL-1 and Skin
 Kidney Antibodies  >3 publications with IL-1 and Kidney
 Placenta Antibodies  >2 publications with IL-1 and Placenta
 Intestine Antibodies  >2 publications with IL-1 and Intestine
 Lymph Node Antibodies  >2 publications with IL-1 and Lymph Node
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