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IL-10 elisa kit :: Chicken Interleukin 10 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS735324
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 10 (IL-10), ELISA Kit

 Also Known As   

Chicken Interleukin 10 ELISA Kit

 Product Gene Name   

IL-10 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Chromosome Location    NC_004002.1 (2243..2749)
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 Species Reactivity    Chicken
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
 Intended Uses    This IL-10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken IL-10. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact technical support.
 Other Notes    Small volumes of IL-10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-10 purchase    MBS735324 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 10 (IL-10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-10, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS735324 kit is based on IL-10 antibody-IL-10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-10. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL-10 elisa kit

   Principle of the Assay: IL-10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-10 antibody and an IL-10-HRP conjugate. The assay sample and buffer are incubated together with IL-10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-10 concentration since IL-10 from samples and IL-10-HRP conjugate compete for the anti-IL-10 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-10 from the sample, fewer sites are left to bind IL-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-10 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for IL-10 elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of IL-10 elisa kit    IL-10 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS735324
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NCBI/Uniprot data below describe general gene information for IL-10. It may not necessarily be applicable to this product.
 NCBI GI #    21492460
 NCBI GeneID    944684
 NCBI Accession #    NP_659579.1 [Other Products]
 NCBI GenBank Nucleotide #    NC_004002.1 [Other Products]
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 NCBI Official Full Name    IL-10
 NCBI Official Symbol    SPPV_03 [Similar Products]
 NCBI Protein Information    IL-10
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Chicken Interleukin 10 ELISA Kit
 Catalog #  Product Name  Catalog #  Product Name
 MBS623629  Interleukin 10 Receptor alpha (IL10RA, IL-10RA, IL-10R-A, CDw210a, HIL-10R, IL-10R1, IL10R)  MBS650805  Interleukin 10, Recombinant, Rat (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
 MBS102670  Cat Interleukin 10 ELISA Kit  MBS2089642  Interleukin 10 (IL10) Antibody Pair
 MBS282130  Fish Interleukin 10 (IL-10) ELISA Kit  MBS2014655  Polyclonal Antibody to Interleukin 10 (IL10)
 MBS2036264  Cy3-Linked Polyclonal Antibody to Interleukin 10 (IL10)  MBS755920  Canine Anti-Interleukin 10 ELISA Kit
 MBS2057347  Cy3-Linked Polyclonal Antibody to Interleukin 10 Receptor Beta (IL10Rb)  MBS703712  Bovine Interleukin 10, IL-10 ELISA Kit
Organs/Tissues associated with IL-10 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with IL-10 and Bone
 Connective Tissue Antibodies  >4 publications with IL-10 and Connective Tissue
 Heart Antibodies  >3 publications with IL-10 and Heart
 Muscle Antibodies  >3 publications with IL-10 and Muscle
 Brain Antibodies  >3 publications with IL-10 and Brain
 Vascular Antibodies  >2 publications with IL-10 and Vascular
 Embryonic Tissue Antibodies  >2 publications with IL-10 and Embryonic Tissue
 Ear Antibodies  >1 publications with IL-10 and Ear
 Kidney Antibodies  >1 publications with IL-10 and Kidney
 Umbilical Cord Antibodies  >1 publications with IL-10 and Umbilical Cord
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