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IL-1beta elisa kit :: Chicken Interleukin 1beta, IL-1beta ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703082
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $475 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,140 +6 FREE 8GB USB
 
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 Product Name   

Interleukin 1beta, IL-1beta, ELISA Kit

★Popular Item★
 Also Known As   

Chicken Interleukin 1beta, IL-1beta ELISA Kit

 Product Synonym Names    Chicken Interleukin 1beta; IL-1beta ELISA Kit; Interleukin 1beta; IL-1beta
 Product Gene Name   

IL-1beta elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703082 Testing Data    Testing Data PDF
 OMIM    147720
 3D Structure    ModBase 3D Structure for P01584
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 Species Reactivity    Chicken
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 Specificity    This assay has high sensitivity and excellent specificity for detection of chicken IL-1beta. No significant cross-reactivity or interference between chicken IL-1beta and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Competitive
 Detection Range    0. 27 pg/ml -200 pg/ml.
 Sensitivity    The minimum detectable dose of chicken IL-1beta is typically less than 0.27 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest chicken IL-1beta concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-1beta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-1beta purchase    MBS703082 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 1beta, IL-1beta, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0. 27 pg/ml -200 pg/ml in biological research samples containing IL-1beta, with an estimated sensitivity of The minimum detectable dose of chicken IL-1beta is typically less than 0.27 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest chicken IL-1beta concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS703082 kit is based on IL-1beta antibody-IL-1beta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-1beta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-1beta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-1beta elisa kit

   Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IL-1beta. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody specific to IL-1beta. The competitive inhibition reaction is launched between with pre-coated IL-1beta and IL-1beta in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of IL-1beta in the sample. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of IL-1beta elisa kit    IL-1beta elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703082. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-1beta. It may not necessarily be applicable to this product.
 NCBI GI #    10835145
 NCBI GeneID    3553
 NCBI Accession #    NP_000567.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000576.2 [Other Products]
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 UniProt Primary Accession #    P01584 [Other Products]
 UniProt Secondary Accession #    Q53X59; Q53XX2; Q7M4S7; Q7RU01; Q96HE5; Q9UCT6 [Other Products]
 UniProt Related Accession #    P01584 [Other Products]
 Molecular Weight    30,748 Da
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 NCBI Official Full Name    interleukin-1 beta proprotein
 NCBI Official Synonym Full Names    interleukin 1, beta
 NCBI Official Symbol    IL1B [Similar Products]
 NCBI Official Synonym Symbols   
IL-1; IL1F2; IL1-BETA
[Similar Products]
 NCBI Protein Information    interleukin-1 beta; IL-1 beta; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta
 UniProt Protein Name    Interleukin-1 beta
 UniProt Synonym Protein Names   
Catabolin
 UniProt Gene Name    IL1B [Similar Products]
 UniProt Synonym Gene Names    IL1F2; IL-1 beta [Similar Products]
 UniProt Entry Name    IL1B_HUMAN
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 NCBI Summary for IL-1beta    The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL-1beta    Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.1 PublicationManual assertion based on experiment in:Ref.15
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 Research Articles on IL-1beta    1. Interleukin-1beta promotes hypoxia-induced apoptosis of glioblastoma cells by inhibiting HIF-1 mediated adrenomedullin production.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-1beta elisa kitPathways associated with IL-1beta elisa kit
 Reference Product  PubMed Publications
 IL6 elisa kit  >250 publications with IL-1beta and IL6
 CASP1 elisa kit  >51 publications with IL-1beta and CASP1
 IL1R1 elisa kit  >36 publications with IL-1beta and IL1R1
 NOS2 elisa kit  >25 publications with IL-1beta and NOS2
 IL18 elisa kit  >24 publications with IL-1beta and IL18
 NFKB1 elisa kit  >20 publications with IL-1beta and NFKB1
 MYD88 elisa kit  >19 publications with IL-1beta and MYD88
 IL1R2 elisa kit  >12 publications with IL-1beta and IL1R2
 IL23A elisa kit  >5 publications with IL-1beta and IL23A
 IL1RAP elisa kit  >3 publications with IL-1beta and IL1RAP
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 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Cellular Roles Of Anthrax Toxin Pathway antibodies  Cellular Roles Of Anthrax Toxin Pathway Diagram
Diseases associated with IL-1beta elisa kitOrgans/Tissues associated with IL-1beta elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >345 publications with IL-1beta and Inflammation
 Necrosis Antibodies  >215 publications with IL-1beta and Necrosis
 Skin Diseases Antibodies  >46 publications with IL-1beta and Skin Diseases
 Stomach Neoplasms Antibodies  >41 publications with IL-1beta and Stomach Neoplasms
 Arthritis, Rheumatoid Antibodies  >26 publications with IL-1beta and Arthritis, Rheumatoid
 Adenocarcinoma Antibodies  >23 publications with IL-1beta and Adenocarcinoma
 Hypersensitivity Antibodies  >19 publications with IL-1beta and Hypersensitivity
 Myocardial Ischemia Antibodies  >19 publications with IL-1beta and Myocardial Ischemia
 Asthma Antibodies  >19 publications with IL-1beta and Asthma
 Colitis Antibodies  >16 publications with IL-1beta and Colitis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >162 publications with IL-1beta and Blood
 Lung Antibodies  >36 publications with IL-1beta and Lung
 Stomach Antibodies  >33 publications with IL-1beta and Stomach
 Uterus Antibodies  >28 publications with IL-1beta and Uterus
 Vascular Antibodies  >23 publications with IL-1beta and Vascular
 Brain Antibodies  >18 publications with IL-1beta and Brain
 Liver Antibodies  >18 publications with IL-1beta and Liver
 Skin Antibodies  >15 publications with IL-1beta and Skin
 Mouth Antibodies  >13 publications with IL-1beta and Mouth
 Bone Marrow Antibodies  >10 publications with IL-1beta and Bone Marrow
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