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IL-33 elisa kit :: Chicken Interleukin 33 (IL-33) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS266987
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $315 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 33 (IL-33), ELISA Kit

 Also Known As   

Chicken Interleukin 33 (IL-33) ELISA Kit

 Product Gene Name   

IL-33 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    608678
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 Species Reactivity    Chicken
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 Specificity    No cross-reaction with other factors.
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 Samples    Chicken serum, plasma or cell culture supernatant and organizations
 Assay Type    Quantitative Sandwich
 Detection Range    1000 pg/ml-15.6 pg/ml
 Sensitivity    5 pg/ml
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of IL-33 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-33 purchase    MBS266987 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 33 (IL-33) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1000 pg/ml-15.6 pg/ml in biological research samples containing IL-33, with an estimated sensitivity of 5 pg/ml. The ELISA analytical biochemical technique of the MBS266987 kit is based on IL-33 antibody-IL-33 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-33 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-33. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Chicken serum, plasma or cell culture supernatant and organizations. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL-33 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken IL-33 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of IL-33 elisa kit    IL-33 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS266987
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NCBI/Uniprot data below describe general gene information for IL-33. It may not necessarily be applicable to this product.
 NCBI GI #    314122337
 NCBI GeneID    90865
 NCBI Accession #    NP_001186570.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001199641.1 [Other Products]
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 UniProt Secondary Accession #    Q2YEJ5; B2R8L1; B4DJ35; B4E1Q9; D3DRI5; E7EAX4 [Other Products]
 UniProt Related Accession #    O95760 [Other Products]
 Molecular Weight    16,250 Da
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 NCBI Official Full Name    interleukin-33 isoform 3
 NCBI Official Synonym Full Names    interleukin 33
 NCBI Official Symbol    IL33 [Similar Products]
 NCBI Official Synonym Symbols   
DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
[Similar Products]
 NCBI Protein Information    interleukin-33; IL-33; IL-1F11; DVS27-related protein; interleukin-1 family member 11; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules
 UniProt Protein Name    Interleukin-33
 UniProt Synonym Protein Names   
Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules; NF-HEV
 UniProt Gene Name    IL33 [Similar Products]
 UniProt Synonym Gene Names    C9orf26; IL1F11; NFHEV; IL-33; IL-1F11; NF-HEV [Similar Products]
 UniProt Entry Name    IL33_HUMAN
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 NCBI Summary for IL-33    IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]
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 UniProt Comments for IL-33    IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular space; transport vesicle; chromosome; nucleus

Molecular Function: protein binding; cytokine activity

Biological Process: positive regulation of T-helper 2 type immune response; negative regulation of T-helper 1 type immune response; T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; positive regulation of interleukin-5 production; positive regulation of inflammatory response; positive regulation of macrophage activation
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 Research Articles on IL-33    1. Hypoxia-inducible factor-1alpha and interleukin 33 form a regulatory circuit to perpetuate the inflammation in rheumatoid arthritis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-33 elisa kitPathways associated with IL-33 elisa kit
 Reference Product  PubMed Publications
 IL1RL1 elisa kit  >94 publications with IL-33 and IL1RL1
 CASP1 elisa kit  >15 publications with IL-33 and CASP1
 IL18 elisa kit  >13 publications with IL-33 and IL18
 IL25 elisa kit  >9 publications with IL-33 and IL25
 TSLP elisa kit  >8 publications with IL-33 and TSLP
 IL18R1 elisa kit  >4 publications with IL-33 and IL18R1
 CSF2 elisa kit  >1 publications with IL-33 and CSF2
 Products by Pathway  Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
Diseases associated with IL-33 elisa kitOrgans/Tissues associated with IL-33 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >112 publications with IL-33 and Inflammation
 Asthma Antibodies  >57 publications with IL-33 and Asthma
 Hypersensitivity, Immediate Antibodies  >55 publications with IL-33 and Hypersensitivity, Immediate
 Lung Diseases Antibodies  >51 publications with IL-33 and Lung Diseases
 Necrosis Antibodies  >36 publications with IL-33 and Necrosis
 Cardiovascular Diseases Antibodies  >26 publications with IL-33 and Cardiovascular Diseases
 Heart Diseases Antibodies  >14 publications with IL-33 and Heart Diseases
 Neoplasms Antibodies  >14 publications with IL-33 and Neoplasms
 Dermatitis, Atopic Antibodies  >10 publications with IL-33 and Dermatitis, Atopic
 Hypertrophy Antibodies  >9 publications with IL-33 and Hypertrophy
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >29 publications with IL-33 and Lung
 Heart Antibodies  >22 publications with IL-33 and Heart
 Skin Antibodies  >21 publications with IL-33 and Skin
 Vascular Antibodies  >19 publications with IL-33 and Vascular
 Muscle Antibodies  >14 publications with IL-33 and Muscle
 Liver Antibodies  >12 publications with IL-33 and Liver
 Intestine Antibodies  >10 publications with IL-33 and Intestine
 Brain Antibodies  >6 publications with IL-33 and Brain
 Connective Tissue Antibodies  >5 publications with IL-33 and Connective Tissue
 Eye Antibodies  >5 publications with IL-33 and Eye
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