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IL-6 elisa kit :: Chicken Interleukin 6 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS737945
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 6 (IL-6), ELISA Kit

★Popular Item★
 Also Known As   

Chicken Interleukin 6 ELISA Kit

 Product Gene Name   

IL-6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 4; NC_005103.3 (3095536..3100112). Location: 4q11
 3D Structure    ModBase 3D Structure for P20607
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 Species Reactivity    Chicken
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IL-6. No significant cross-reactivity or interference between IL-6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-6 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
 Intended Uses    This IL-6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken IL-6. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-6 purchase    MBS737945 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 6 (IL-6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-6, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS737945 kit is based on IL-6 antibody-IL-6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL-6 elisa kit

   Principle of the assay: IL-6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-6 antibody and an IL-6-HRP conjugate. The assay sample and buffer are incubated together with IL-6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-6 concentration since IL-6 from samples and IL-6-HRP conjugate compete for the anti-IL-6 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-6 from the sample, fewer sites are left to bind IL-6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-6 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for IL-6 elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of IL-6 elisa kit    IL-6 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS737945. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-6. It may not necessarily be applicable to this product.
 NCBI GI #    7549769
 NCBI GeneID    24498
 NCBI Accession #    NP_036721.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012589.1 [Other Products]
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 UniProt Primary Accession #    P20607 [Other Products]
 UniProt Related Accession #    P20607 [Other Products]
 Molecular Weight    24,357 Da
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 NCBI Official Full Name    interleukin-6
 NCBI Official Synonym Full Names    interleukin 6
 NCBI Official Symbol    Il6 [Similar Products]
 NCBI Official Synonym Symbols   
ILg6; Ifnb2
[Similar Products]
 NCBI Protein Information    interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)
 UniProt Protein Name    Interleukin-6
 UniProt Gene Name    Il6 [Similar Products]
 UniProt Synonym Gene Names    Il-6; IL-6 [Similar Products]
 UniProt Entry Name    IL6_RAT
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 NCBI Summary for IL-6    a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]
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 UniProt Comments for IL-6    Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance

By similarity.

Subcellular location: Secreted.

Sequence similarities: Belongs to the IL-6 superfamily.
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 Research Articles on IL-6    1. Our study provides for the first time in vivo electrophysiological evidence that meningeal action of IL-1beta can promote the activation and increased mechanosensitivity of intracranial meningeal nociceptors and that IL-6 generally lacks these properties.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IL-6 elisa kitDiseases associated with IL-6 elisa kit
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1308 publications with IL-6 and Inflammation
 Necrosis Antibodies  >1109 publications with IL-6 and Necrosis
 Neoplasms Antibodies  >622 publications with IL-6 and Neoplasms
 Cardiovascular Diseases Antibodies  >555 publications with IL-6 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >272 publications with IL-6 and Nervous System Diseases
 Heart Diseases Antibodies  >210 publications with IL-6 and Heart Diseases
 Lung Diseases Antibodies  >207 publications with IL-6 and Lung Diseases
 Obesity Antibodies  >154 publications with IL-6 and Obesity
 Atherosclerosis Antibodies  >154 publications with IL-6 and Atherosclerosis
 Liver Diseases Antibodies  >148 publications with IL-6 and Liver Diseases
Organs/Tissues associated with IL-6 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Uncharacterized Tissue Antibodies  >1 publications with IL-6 and Uncharacterized Tissue
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