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PAR3 elisa kit :: Chicken PAR3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2502608
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   


 Also Known As   

Chicken PAR3 (Protease Activated Receptor 3) ELISA Kit

 Product Gene Name   

PAR3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606745
 3D Structure    ModBase 3D Structure for Q8TEW0
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 Species Reactivity    Chicken
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 Specificity    This kit recognizes natural and recombinant Chicken PAR3. No significant cross-reactivity or interference between Chicken PAR3 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    1.563-100ng/mL
 Sensitivity    Min: 0.938ng/mL; Max: 100ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PAR3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PAR3 purchase    MBS2502608 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PAR3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.563-100ng/mL in biological research samples containing PAR3, with an estimated sensitivity of Min: 0.938ng/mL; Max: 100ng/mL. The ELISA analytical biochemical technique of the MBS2502608 kit is based on PAR3 antibody-PAR3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PAR3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PAR3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PAR3 elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken PAR3 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PAR3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PAR3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PAR3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PAR3. You can calculate the concentration of PAR3 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PAR3 elisa kit    PAR3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2502608. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PAR3. It may not necessarily be applicable to this product.
 NCBI GI #    296278259
 NCBI GeneID    56288
 NCBI Accession #    NP_001171716.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001184787.1 [Other Products]
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 UniProt Primary Accession #    Q8TEW0 [Other Products]
 UniProt Secondary Accession #    Q5T2U1; Q5VUA2; Q5VUA3; Q5VWV0; Q5VWV1; Q5VWV3; Q5VWV4; Q5VWV5; Q6IQ47; Q8TCZ9; F5H5T0 [Other Products]
 UniProt Related Accession #    Q8TEW0 [Other Products]
 Molecular Weight    149,695 Da
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 NCBI Official Full Name    partitioning defective 3 homolog isoform 4
 NCBI Official Synonym Full Names    par-3 family cell polarity regulator
 NCBI Official Symbol    PARD3 [Similar Products]
 NCBI Official Synonym Symbols   
Baz; ASIP; PAR3; PARD-3; PARD3A; SE2-5T2; PPP1R118; SE2-5L16; SE2-5LT1; PAR3alpha
[Similar Products]
 NCBI Protein Information    partitioning defective 3 homolog; CTCL tumor antigen se2-5; PAR3-alpha; atypical PKC isotype-specific interacting protein; atypical PKC isotype-specific-interacting protein; bazooka; par-3 family cell polarity regulator alpha; par-3 partitioning defective 3 homolog; protein phosphatase 1, regulatory subunit 118
 UniProt Protein Name    Partitioning defective 3 homolog
 UniProt Synonym Protein Names   
Atypical PKC isotype-specific-interacting protein; ASIP; CTCL tumor antigen se2-5; PAR3-alpha
 UniProt Gene Name    PARD3 [Similar Products]
 UniProt Synonym Gene Names    PAR3; PAR3A; PAR-3; PARD-3; ASIP [Similar Products]
 UniProt Entry Name    PARD3_HUMAN
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 NCBI Summary for PAR3    This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]
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 UniProt Comments for PAR3    PAR3-alpha: an adapter protein involved in asymmetrical cell division, and cell polarization processes. Polarity complex proteins, including PARD3, are essential for PIP3-induced receptor tyrosine kinase recycling. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Association with PARD6B may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons. PDZ 1 domain modulates interactions with JAM1, PARD6A and PARD6B; PDZ 2 domain mediates interaction with membranes containing phosphoinositol lipids; PDZ 3 domain regulates interactions with PTEN (via C-terminus). Belongs to the PAR3 family. 10 isoforms of the human protein are produced by alternative splicing. Isoform 2, but not at least isoform 3 interacts with PRKCZ.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p11.21

Cellular Component: internode region of axon; cell-cell adherens junction; protein complex; tight junction; cell soma; apical part of cell; plasma membrane; endomembrane system; spindle; intercellular junction; cell cortex; cytosol; cell junction

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; protein kinase C binding; protein phosphatase binding; phosphatidylinositol 3-phosphate binding

Biological Process: positive regulation of myelination; intercellular junction assembly and maintenance; regulation of actin filament-based process; axonogenesis; centrosome localization; asymmetric cell division; transforming growth factor beta receptor signaling pathway; protein kinase C activation; establishment and/or maintenance of cell polarity; protein complex assembly; microtubule cytoskeleton organization and biogenesis; protein targeting to membrane; cell cycle; myelination in the peripheral nervous system
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 Research Articles on PAR3    1. A cytoplasmic expression of PAR-3 is therefore implicated in worse clinical and pathological cancer features in clear cell renal cell carcinoma and could be useful to identify patients with high-risk tumors.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PAR3 elisa kitDiseases associated with PAR3 elisa kit
 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Cell-cell Junction Organization Pathway antibodies  Cell-cell Junction Organization Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >12 publications with PAR3 and Carcinoma
 Nervous System Diseases Antibodies  >7 publications with PAR3 and Nervous System Diseases
 Cell Transformation, Neoplastic Antibodies  >7 publications with PAR3 and Cell Transformation, Neoplastic
 Congenital Abnormalities Antibodies  >6 publications with PAR3 and Congenital Abnormalities
 Adenocarcinoma Antibodies  >5 publications with PAR3 and Adenocarcinoma
 Kidney Diseases Antibodies  >5 publications with PAR3 and Kidney Diseases
 Liver Neoplasms Antibodies  >4 publications with PAR3 and Liver Neoplasms
 Inflammation Antibodies  >4 publications with PAR3 and Inflammation
 Necrosis Antibodies  >3 publications with PAR3 and Necrosis
 Liver Diseases Antibodies  >2 publications with PAR3 and Liver Diseases
Organs/Tissues associated with PAR3 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >24 publications with PAR3 and Brain
 Kidney Antibodies  >21 publications with PAR3 and Kidney
 Blood Antibodies  >16 publications with PAR3 and Blood
 Eye Antibodies  >14 publications with PAR3 and Eye
 Vascular Antibodies  >12 publications with PAR3 and Vascular
 Nerve Antibodies  >11 publications with PAR3 and Nerve
 Liver Antibodies  >8 publications with PAR3 and Liver
 Embryonic Tissue Antibodies  >7 publications with PAR3 and Embryonic Tissue
 Skin Antibodies  >7 publications with PAR3 and Skin
 Mammary Gland Antibodies  >6 publications with PAR3 and Mammary Gland
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