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TEF elisa kit :: Chicken TEF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2510984 TEF elisa kit
Unit / Price
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TEF, ELISA Kit

 Also Known As   

Chicken TEF (Thyrotroph Embryonic Factor) ELISA Kit

 Product Gene Name   

TEF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    188595
 3D Structure    ModBase 3D Structure for Q10587
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 Species Reactivity    Chicken
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 Specificity    This kit recognizes natural and recombinant Chicken TEF. No significant cross-reactivity or interference between Chicken TEF and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    31.25-2000pg/mL
 Sensitivity    18.75pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TEF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TEF purchase    MBS2510984 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TEF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000pg/mL in biological research samples containing TEF, with an estimated sensitivity of 18.75pg/mL. The ELISA analytical biochemical technique of the MBS2510984 kit is based on TEF antibody-TEF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TEF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TEF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TEF elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken TEF concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TEF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TEF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TEF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TEF. You can calculate the concentration of TEF in the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2510984. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TEF. It may not necessarily be applicable to this product.
 NCBI GI #    4507431
 NCBI GeneID    7008
 NCBI Accession #    NP_003207.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003216.3 [Other Products]
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 UniProt Primary Accession #    Q10587 [Other Products]
 UniProt Secondary Accession #    Q15729; Q7Z3J7; Q8IU94; Q96TG4; B0QYS8; B2RC22 [Other Products]
 UniProt Related Accession #    Q10587 [Other Products]
 Molecular Weight    30,635 Da
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 NCBI Official Full Name    thyrotroph embryonic factor isoform 1
 NCBI Official Synonym Full Names    thyrotrophic embryonic factor
 NCBI Official Symbol    TEF [Similar Products]
 NCBI Protein Information    thyrotroph embryonic factor
 UniProt Protein Name    Thyrotroph embryonic factor
 Protein Family    Transcription factor
 UniProt Gene Name    TEF [Similar Products]
 UniProt Synonym Gene Names    KIAA1655 [Similar Products]
 UniProt Entry Name    TEF_HUMAN
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 NCBI Summary for TEF    This gene encodes a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. It is expressed in a broad range of cells and tissues in adult animals, however, during embryonic development, TEF expression appears to be restricted to the developing anterior pituitary gland, coincident with the appearance of thyroid-stimulating hormone, beta (TSHB). Indeed, TEF can bind to, and transactivate the TSHB promoter. It shows homology (in the functional domains) with other members of the PAR-bZIP subfamily of transcription factors, which include albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP); VBP is considered the chicken homologue of TEF. Different members of the subfamily can readily form heterodimers, and share DNA-binding, and transcriptional regulatory properties. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
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 UniProt Comments for TEF    TEF: Transcription factor that binds to and transactivates the TSHB promoter. Binds to a minimal DNA-binding sequence 5'- [TC][AG][AG]TTA[TC][AG]-3'. Belongs to the bZIP family. PAR subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleus

Molecular Function: protein homodimerization activity; sequence-specific DNA binding; protein heterodimerization activity; double-stranded DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; rhythmic process; positive regulation of transcription from RNA polymerase II promoter
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 Research Articles on TEF    1. findings show the polymorphisms of Cry1 rs2287161 and Tef rs738499 are associated to major depressive disorder in the Chinese population
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TEF elisa kitDiseases associated with TEF elisa kit
 Reference Product  PubMed Publications
 TEAD1 elisa kit  >92 publications with TEF and TEAD1
 DBP elisa kit  >40 publications with TEF and DBP
 TEAD4 elisa kit  >15 publications with TEF and TEAD4
 TEAD3 elisa kit  >13 publications with TEF and TEAD3
 TEAD2 elisa kit  >9 publications with TEF and TEAD2
 VGLL2 elisa kit  >4 publications with TEF and VGLL2
 PDXK elisa kit  >1 publications with TEF and PDXK
 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >280 publications with TEF and Congenital Abnormalities
 Heart Diseases Antibodies  >43 publications with TEF and Heart Diseases
 Lung Diseases Antibodies  >40 publications with TEF and Lung Diseases
 Disease Models, Animal Antibodies  >35 publications with TEF and Disease Models, Animal
 Weight Loss Antibodies  >35 publications with TEF and Weight Loss
 Hypertrophy Antibodies  >27 publications with TEF and Hypertrophy
 Heart Defects, Congenital Antibodies  >23 publications with TEF and Heart Defects, Congenital
 Inflammation Antibodies  >23 publications with TEF and Inflammation
 Weight Gain Antibodies  >22 publications with TEF and Weight Gain
 Necrosis Antibodies  >22 publications with TEF and Necrosis
Organs/Tissues associated with TEF elisa kit
 Organ/Tissue Name  Pubmed Publications
 Esophagus Antibodies  >190 publications with TEF and Esophagus
 Muscle Antibodies  >161 publications with TEF and Muscle
 Trachea Antibodies  >114 publications with TEF and Trachea
 Liver Antibodies  >94 publications with TEF and Liver
 Lung Antibodies  >93 publications with TEF and Lung
 Embryonic Tissue Antibodies  >69 publications with TEF and Embryonic Tissue
 Brain Antibodies  >48 publications with TEF and Brain
 Thyroid Antibodies  >42 publications with TEF and Thyroid
 Bone Antibodies  >39 publications with TEF and Bone
 Kidney Antibodies  >38 publications with TEF and Kidney
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