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VEGF elisa kit :: Chicken Vascular Endothelial Growth Factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS735777
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Vascular Endothelial Growth Factor (VEGF), ELISA Kit

★Popular Item★
 Also Known As   

Chicken Vascular Endothelial Growth Factor ELISA Kit

 Product Gene Name   

VEGF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 6; NC_000006.11 (43737946..43754224). Location: 6p12
 OMIM    192240
 3D Structure    ModBase 3D Structure for P15692
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 Species Reactivity    Chicken
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 Specificity    This assay has high sensitivity and excellent specificity for detection of VEGF. No significant cross-reactivity or interference between VEGFand analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VEGFand all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of VEGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VEGF purchase    MBS735777 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Vascular Endothelial Growth Factor (VEGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VEGF, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS735777 kit is based on VEGF antibody-VEGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VEGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VEGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for VEGF elisa kit

   Intended Uses: This VEGFELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken VEGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: VEGFELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-VEGFantibody and an VEGF-HRP conjugate. The assay sample and buffer are incubated together with VEGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VEGFconcentration since VEGFfrom samples and VEGF-HRP conjugate compete for the anti-VEGFantibody binding site. Since the number of sites is limited, as more sites are occupied by VEGFfrom the sample, fewer sites are left to bind VEGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGFconcentration in each sample is interpolated from this standard curve.
 Product Categories/Family for VEGF elisa kit    Cancer
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 Typical Testing Data/Standard Curve (for reference only) of VEGF elisa kit    VEGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS735777. Click to request current manual
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NCBI/Uniprot data below describe general gene information for VEGF. It may not necessarily be applicable to this product.
 NCBI GI #    181971
 NCBI GeneID    7422
 NCBI Accession #    AAA35789.1 [Other Products]
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 UniProt Primary Accession #    P15692 [Other Products]
 UniProt Secondary Accession #    O60720; O75875; Q074Z4; Q16889; Q5UB46; Q6P0P5; Q96KJ0; Q96L82; Q96NW5; Q9H1W8; B5BU86 [Other Products]
 UniProt Related Accession #    P15692; Q59FH5; Q6WZM0; Q71S09; Q96FD9; Q9UNS8 [Other Products]
 Molecular Weight    27,042 Da
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 NCBI Official Full Name    vascular endothelial growth factor
 NCBI Official Synonym Full Names    vascular endothelial growth factor A
 NCBI Official Symbol    VEGFA [Similar Products]
 NCBI Official Synonym Symbols   
VPF; VEGF; MVCD1
[Similar Products]
 NCBI Protein Information    vascular endothelial growth factor A; vascular permeability factor
 UniProt Protein Name    Vascular endothelial growth factor A
 UniProt Synonym Protein Names   
Vascular permeability factor
 Protein Family    Vascular endothelial growth factor
 UniProt Gene Name    VEGFA [Similar Products]
 UniProt Synonym Gene Names    VEGF; VEGF-A; VPF [Similar Products]
 UniProt Entry Name    VEGFA_HUMAN
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 NCBI Summary for VEGF    This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for VEGF    VEGF iso14: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the PDGF/VEGF growth factor family. 13 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted; Secreted, signal peptide; Cytokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; membrane; cytoplasm; extracellular region; secretory granule

Molecular Function: heparin binding; identical protein binding; protein homodimerization activity; growth factor activity; extracellular matrix binding; platelet-derived growth factor receptor binding; cytokine activity; vascular endothelial growth factor receptor 1 binding; vascular endothelial growth factor receptor binding; receptor agonist activity; protein binding; vascular endothelial growth factor receptor 2 binding; protein heterodimerization activity; fibronectin binding; chemoattractant activity

Biological Process: heart morphogenesis; positive regulation of positive chemotaxis; positive regulation of cell adhesion; macrophage differentiation; positive regulation of leukocyte migration; positive regulation of receptor internalization; cell maturation; basophil chemotaxis; positive regulation of MAP kinase activity; regulation of cell shape; positive chemotaxis; positive regulation of mesenchymal cell proliferation; mesoderm development; kidney development; platelet activation; nervous system development; positive regulation of neuroblast proliferation; monocyte differentiation; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; positive regulation of protein amino acid autophosphorylation; positive regulation of vascular permeability; regulation of transcription from RNA polymerase II promoter; patterning of blood vessels; eye photoreceptor cell development; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; camera-type eye morphogenesis; cell migration during sprouting angiogenesis; branching morphogenesis of a tube; cardiac muscle fiber development; positive regulation of cell division; positive regulation of axon extension involved in axon guidance; activation of protein kinase activity; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of cGMP metabolic process; surfactant homeostasis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; lactation; post-embryonic camera-type eye development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of vascular endothelial growth factor receptor signaling pathway; induction of positive chemotaxis; platelet degranulation; ovarian follicle development; positive regulation of focal adhesion formation; epithelial cell differentiation; positive regulation of cell proliferation; angiogenesis; vasculogenesis; in utero embryonic development; lumen formation; positive regulation of cell motility; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein complex assembly; response to hypoxia; artery morphogenesis; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; growth; positive regulation of cell migration; lung development

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 1
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 Research Articles on VEGF    1. The genetic polymorphism +12916C in VEGF genes were correlated with MTA1 overexpression in hepatocellular carcinoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with VEGF elisa kitPathways associated with VEGF elisa kit
 Reference Product  PubMed Publications
 HIF1A elisa kit  >324 publications with VEGF and HIF1A
 KDR elisa kit  >202 publications with VEGF and KDR
 FLT1 elisa kit  >104 publications with VEGF and FLT1
 NRP1 elisa kit  >36 publications with VEGF and NRP1
 VEGFB elisa kit  >34 publications with VEGF and VEGFB
 CTGF elisa kit  >18 publications with VEGF and CTGF
 AKT1 elisa kit  >17 publications with VEGF and AKT1
 PGF elisa kit  >16 publications with VEGF and PGF
 NRP2 elisa kit  >12 publications with VEGF and NRP2
 SEMA3F elisa kit  >5 publications with VEGF and SEMA3F
 Products by Pathway  Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Glypican 1 Network Pathway antibodies  Glypican 1 Network Pathway Diagram
Diseases associated with VEGF elisa kitOrgans/Tissues associated with VEGF elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1902 publications with VEGF and Neoplasms
 Neovascularization, Pathologic Antibodies  >1274 publications with VEGF and Neovascularization, Pathologic
 Cardiovascular Diseases Antibodies  >771 publications with VEGF and Cardiovascular Diseases
 Macular Degeneration Antibodies  >537 publications with VEGF and Macular Degeneration
 Adenocarcinoma Antibodies  >412 publications with VEGF and Adenocarcinoma
 Gastrointestinal Diseases Antibodies  >345 publications with VEGF and Gastrointestinal Diseases
 Choroidal Neovascularization Antibodies  >319 publications with VEGF and Choroidal Neovascularization
 Neoplasm Metastasis Antibodies  >313 publications with VEGF and Neoplasm Metastasis
 Nervous System Diseases Antibodies  >277 publications with VEGF and Nervous System Diseases
 Edema Antibodies  >274 publications with VEGF and Edema
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >4971 publications with VEGF and Vascular
 Blood Antibodies  >1982 publications with VEGF and Blood
 Eye Antibodies  >811 publications with VEGF and Eye
 Bone Antibodies  >335 publications with VEGF and Bone
 Lung Antibodies  >329 publications with VEGF and Lung
 Muscle Antibodies  >325 publications with VEGF and Muscle
 Embryonic Tissue Antibodies  >305 publications with VEGF and Embryonic Tissue
 Heart Antibodies  >245 publications with VEGF and Heart
 Kidney Antibodies  >215 publications with VEGF and Kidney
 Liver Antibodies  >196 publications with VEGF and Liver
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