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| Product Name
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polybromo-1 (PBRM1), ELISA Kit
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| Also Known As
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Chicken Protein polybromo-1 (PBRM1) ELISA Kit
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| Product Synonym Names
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BAF180; MGC156155; MGC156156; PB1;
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Request for Current Manual Insert
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| 3D Structure
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ModBase 3D Structure for Q90941
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| Species Reactivity
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Chicken
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of Chicken PBRM1. No significant cross-reactivity or interference between Chicken PBRM1 and analogues was observed.
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| Samples
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Serum, Plasma, Other biological fluids
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| Assay Type
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Sandwich
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| Sample Volume
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1-200 uL
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| Precision
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Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
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| Detection Wavelength
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450 nm
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| Preparation and Storage
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Store at 2-8 degree C.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of PBRM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for PBRM1 purchase
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MBS284201 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the polybromo-1 (PBRM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PBRM1. The ELISA analytical biochemical technique of the MBS284201 kit is based on PBRM1 antibody-PBRM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PBRM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PBRM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PBRM1 elisa kit
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Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PBRM1 in samples. An antibody specific for PBRM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPBRM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PBRM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PBRM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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| NCBI/Uniprot data below describe general gene information for PBRM1. It may not necessarily be applicable to this product. |
| NCBI GI #
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45384026
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| NCBI GeneID
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396074
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| NCBI Accession #
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NP_990496.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_205165.1
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[Other Products]
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| UniProt Primary Accession #
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Q90941
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[Other Products]
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| UniProt Related Accession #
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Q90941
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[Other Products]
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| Molecular Weight
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187,055 Da
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| NCBI Official Full Name
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protein polybromo-1
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| NCBI Official Synonym Full Names
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polybromo 1
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| NCBI Official Symbol
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PBRM1
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[Similar Products]
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| NCBI Official Synonym Symbols
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PB1
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[Similar Products]
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| NCBI Protein Information
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protein polybromo-1
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| UniProt Protein Name
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Protein polybromo-1
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| Protein Family
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Protein polybromo
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| UniProt Gene Name
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PBRM1
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[Similar Products]
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| UniProt Synonym Gene Names
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PB1
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[Similar Products]
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| UniProt Comments for PBRM1
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Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Diseases associated with PBRM1 elisa kit | Organs/Tissues associated with PBRM1 elisa kit |
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ELISA Kit 
