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PKLR elisa kit :: Dog pyruvate kinase, liver and RBC ELISA Kit

Scan QR to view Datasheet Catalog #    MBS920482
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

pyruvate kinase, liver and RBC (PKLR), ELISA Kit

★Popular Item★
 Also Known As   

Dog Pyruvate kinase isozymes R/L, PKLR ELISA Kit

 Product Synonym Names    Dog Pyruvate kinase isozymes R/L (PKLR) ELISA kit; PK1; PKL; PKR; PKRL; RPK; R-type/L-type pyruvate kinase; pyruvate kinase 1; pyruvate kinase isozyme R/L; pyruvate kinase type L; pyruvate kinase; liver and blood cell; red cell/liver pyruvate kinase; pyruvate kinase; liver and RBC
 Product Gene Name   

PKLR elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS920482 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q29536
 Species Reactivity    Dog
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 Specificity    This assay has high sensitivity and excellent specificity for detection of dog PKLR. No significant cross-reactivity or interference between dog PKLR and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    7.81 mU/ml-500 mU/ml.
 Sensitivity    The minimum detectable dose of dog PKLR is typically less than 1.95 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PKLR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PKLR purchase    MBS920482 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the pyruvate kinase, liver and RBC (PKLR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.81 mU/ml-500 mU/ml in biological research samples containing PKLR, with an estimated sensitivity of The minimum detectable dose of dog PKLR is typically less than 1.95 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS920482 kit is based on PKLR antibody-PKLR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PKLR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PKLR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PKLR elisa kit

   Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PKLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PKLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PKLR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PKLR bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of PKLR elisa kit    PKLR elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS920482. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PKLR. It may not necessarily be applicable to this product.
 NCBI GI #    365192553
 NCBI GeneID    490425
 NCBI Accession #    NP_001242947.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001256018.1 [Other Products]
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 UniProt Primary Accession #    Q29536 [Other Products]
 UniProt Secondary Accession #    F1P923; F1PXP8; F1PY57 [Other Products]
 UniProt Related Accession #    Q29536 [Other Products]
 Molecular Weight    62,048 Da
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 NCBI Official Full Name    pyruvate kinase PKLR isoform 2
 NCBI Official Symbol    PKLR [Similar Products]
 NCBI Protein Information    pyruvate kinase PKLR; Pyruvate kinase isozyme R; Pyruvate kinase isozymes R/L; pyruvate kinase isozymes L/R
 UniProt Protein Name    Pyruvate kinase PKLR
 UniProt Synonym Protein Names   
Pyruvate kinase isozymes L/R
 Protein Family    Pyruvate kinase
 UniProt Gene Name    PKLR [Similar Products]
 UniProt Entry Name    KPYR_CANFA
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 UniProt Comments for PKLR    Function: Plays a key role in glycolysis

Catalytic activity: ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor: Magnesium

Enzyme regulation: Allosterically activated by fructose 1,6-bisphosphate
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 Research Articles on PKLR    1. Erythrocytic PK deficiency is caused by different mutations in different dog breeds and causes chronic severe hemolytic anemia, hemosiderosis, and secondary hemochromatosis because of chronic hemolysis and, an as yet unexplained osteosclerosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PKLR elisa kitPathways associated with PKLR elisa kit
 Reference Product  PubMed Publications
 LDHA elisa kit  >2 publications with PKLR and LDHA
 ME1 elisa kit  >1 publications with PKLR and ME1
 LDHB elisa kit  >1 publications with PKLR and LDHB
 ENO2 elisa kit  >1 publications with PKLR and ENO2
 Products by Pathway  Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 ChREBP Activates Metabolic Gene Expression Pathway antibodies  ChREBP Activates Metabolic Gene Expression Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
Diseases associated with PKLR elisa kitOrgans/Tissues associated with PKLR elisa kit
 Disease Name  Pubmed Publications
 Anemia Antibodies  >38 publications with PKLR and Anemia
 Brain Diseases Antibodies  >10 publications with PKLR and Brain Diseases
 Liver Diseases Antibodies  >7 publications with PKLR and Liver Diseases
 Inflammation Antibodies  >5 publications with PKLR and Inflammation
 Disease Models, Animal Antibodies  >5 publications with PKLR and Disease Models, Animal
 Fatty Liver Antibodies  >4 publications with PKLR and Fatty Liver
 Pyruvate Kinase Deficiency of Red Cells Antibodies  >4 publications with PKLR and Pyruvate Kinase Deficiency of Red Cells
 Jaundice Antibodies  >3 publications with PKLR and Jaundice
 Hypertrophy Antibodies  >2 publications with PKLR and Hypertrophy
 Necrosis Antibodies  >2 publications with PKLR and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >27 publications with PKLR and Liver
 Kidney Antibodies  >3 publications with PKLR and Kidney
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