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5HT/ST elisa kit :: Fish 5-hydroxytryptamine/serotonin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS282358 5HT/ST elisa kit
Unit / Price
48-Strip-Wells  /  $465 +1 FREE 8GB USB
96-Strip-Wells  /  $790 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,500 +4 FREE 8GB USB
10x96-Strip-Wells  /  $6,655 +8 FREE 8GB USB
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 Product Name   

5-hydroxytryptamine/serotonin (5HT/ST), ELISA Kit

★Popular Item★
 Also Known As   

Fish 5-hydroxytryptamine/serotonin (5HT/ST) ELISA Kit

 Product Gene Name   

5HT/ST elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Fish
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Fish5HT/ST. No significant cross-reactivity or interference between Fish5HT/ST and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of 5HT/ST elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for 5HT/ST purchase    MBS282358 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-hydroxytryptamine/serotonin (5HT/ST) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 5HT/ST. The ELISA analytical biochemical technique of the MBS282358 kit is based on 5HT/ST antibody-5HT/ST antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 5HT/ST antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 5HT/ST. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for 5HT/ST elisa kit

   Serotonin also called 5-hydroxytryptamine, is primarily found in the gastrointestinal (GI) tract and central nervous system (CNS) of animals. Approximately 80 percent of the human body's total serotonin is located in the enterochromaffin cells in the gut, where it is used to regulate intestinal movements. The remainder is synthesized in serotonergic neurons in the CNS where it has various functions, including the regulation of mood, appetite, sleep, muscle contraction, and some cognitive functions including memory and learning; and in blood platelets where it helps to regulate hemostasis and blood clotting. In addition to animals, serotonin is also found in fungi and plants. 5-hydroxytryptamine(5-HT) is an important neurotransmitter made by neurons in the central nervous system. These functions are mediated by a large number of receptors.After it is released by a neuron, 5-HT activates receptors located on nearby neurons.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate 5HT/ST in samples. An antibody specific for 5HT/ST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any5HT/ST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5HT/ST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5HT/ST bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS282358. Click to request current manual
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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