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NAADP elisa kit :: General Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2000162 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $295 +1 FREE 8GB USB
48-Strip-Wells  /  $560 +1 FREE 8GB USB
96-Strip-Wells  /  $765 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,105 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,600 +7 FREE 8GB USB
 
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 Product Name   

Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP), ELISA Kit

★Popular Item★
 Also Known As   

Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA Kit

 Product Gene Name   

NAADP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    General
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 Specificity    This assay has high sensitivity and excellent specificity for detection of NAADP. No significant cross-reactivity or interference between NAADP and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
 Assay Type    Quantitative Competitive
 Detection Range    123.5-10,000ng/mL
 Sensitivity    <48.7ng/mL.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NAADP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NAADP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NAADP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NAADP purchase    MBS2000162 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 123.5-10,000ng/mL in biological research samples containing NAADP, with an estimated sensitivity of <48.7ng/mL. The ELISA analytical biochemical technique of the MBS2000162 kit is based on NAADP antibody-NAADP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NAADP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NAADP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NAADP elisa kit

   Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of NAADP in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to NAADP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled NAADP analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NAADP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of NAADP in the sample.
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  Typical Testing Data/Standard Curve (for reference only) of NAADP elisa kit    NAADP elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2000162. Click to request current manual
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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