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aFGF elisa kit :: Goat Acidic Fibroblast Growth Factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS742225
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Acidic Fibroblast Growth Factor (aFGF), ELISA Kit

 Also Known As   

Goat Acidic Fibroblast Growth Factor ELISA Kit

 Product Gene Name   

aFGF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    131220
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 Species Reactivity    Goat
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Sensitivity    0.1 ng/mL.
 Intended Uses    This AFGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat AFGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of aFGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for aFGF purchase    MBS742225 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acidic Fibroblast Growth Factor (aFGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing aFGF, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS742225 kit is based on aFGF antibody-aFGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect aFGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, aFGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for aFGF elisa kit

   Principle of the Assay: AFGF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AFGF. Standards or samples are then added to the microtiter plate wells and AFGF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AFGF present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for AFGF are added to each well to "sandwich" the AFGF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AFGF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AFGF concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for aFGF elisa kit    Cancer
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 Typical Testing Data/Standard Curve (for reference only) of aFGF elisa kit    aFGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS742225. Click to request current manual
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NCBI/Uniprot data below describe general gene information for aFGF. It may not necessarily be applicable to this product.
 NCBI GI #    456828
 NCBI GeneID    2246
 NCBI Accession #    AAB29059.1 [Other Products]
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 UniProt Secondary Accession #    P07502; Q16588; B2R5T0; D3DQF2 [Other Products]
 UniProt Related Accession #    P05230 [Other Products]
 Molecular Weight    6,698 Da
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 NCBI Official Full Name    acidic fibroblast growth factor, partial
 NCBI Official Synonym Full Names    fibroblast growth factor 1 (acidic)
 NCBI Official Symbol    FGF1 [Similar Products]
 NCBI Official Synonym Symbols   
AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
[Similar Products]
 NCBI Protein Information    fibroblast growth factor 1; heparin-binding growth factor 1; beta-endothelial cell growth factor; endothelial cell growth factor, beta; endothelial cell growth factor, alpha
 UniProt Protein Name    Fibroblast growth factor 1
 UniProt Synonym Protein Names   
Acidic fibroblast growth factor; aFGF; Endothelial cell growth factor; ECGF; Heparin-binding growth factor 1; HBGF-1
 Protein Family    Acidic fibroblast growth factor intracellular-binding protein
 UniProt Gene Name    FGF1 [Similar Products]
 UniProt Synonym Gene Names    FGFA; FGF-1; aFGF; ECGF; HBGF-1 [Similar Products]
 UniProt Entry Name    FGF1_HUMAN
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 NCBI Summary for aFGF    The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]
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 UniProt Comments for aFGF    FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cytokine; Cell development/differentiation

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; proteinaceous extracellular matrix; extracellular space; extracellular region; nucleolus; cell cortex; cytosol

Molecular Function: heparin binding; protein binding; growth factor activity; Hsp70 protein binding; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; multicellular organismal development; positive regulation of cholesterol biosynthetic process; signal transduction; positive regulation of MAP kinase activity; positive regulation of angiogenesis; induction of an organ; positive regulation of cell division; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of epithelial cell proliferation; positive regulation of cell migration; lung development
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 Research Articles on aFGF    1. Data suggest that the enhanced cell growth was likely due to the electrical stimulation up-regulated secretion of FGF-1 and FGF-2.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with aFGF elisa kitPathways associated with aFGF elisa kit
 Reference Product  PubMed Publications
 FGFR1 elisa kit  >218 publications with aFGF and FGFR1
 FGFR2 elisa kit  >123 publications with aFGF and FGFR2
 FGFR3 elisa kit  >66 publications with aFGF and FGFR3
 FGFR4 elisa kit  >56 publications with aFGF and FGFR4
 S100A13 elisa kit  >31 publications with aFGF and S100A13
 SYT1 elisa kit  >15 publications with aFGF and SYT1
 EGFR elisa kit  >13 publications with aFGF and EGFR
 GRB2 elisa kit  >11 publications with aFGF and GRB2
 FGF23 elisa kit  >7 publications with aFGF and FGF23
 FGFBP1 elisa kit  >5 publications with aFGF and FGFBP1
 Products by Pathway  Pathway Diagram
 Activated Point Mutants Of FGFR2 Pathway antibodies  Activated Point Mutants Of FGFR2 Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
Diseases associated with aFGF elisa kitOrgans/Tissues associated with aFGF elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >136 publications with aFGF and Nervous System Diseases
 Breast Neoplasms Antibodies  >69 publications with aFGF and Breast Neoplasms
 Heart Diseases Antibodies  >62 publications with aFGF and Heart Diseases
 Inflammation Antibodies  >58 publications with aFGF and Inflammation
 Necrosis Antibodies  >57 publications with aFGF and Necrosis
 Myocardial Ischemia Antibodies  >55 publications with aFGF and Myocardial Ischemia
 Neoplasms, Experimental Antibodies  >43 publications with aFGF and Neoplasms, Experimental
 Hyperplasia Antibodies  >39 publications with aFGF and Hyperplasia
 Fibrosis Antibodies  >34 publications with aFGF and Fibrosis
 Cell Transformation, Neoplastic Antibodies  >33 publications with aFGF and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >298 publications with aFGF and Brain
 Muscle Antibodies  >247 publications with aFGF and Muscle
 Nerve Antibodies  >192 publications with aFGF and Nerve
 Embryonic Tissue Antibodies  >167 publications with aFGF and Embryonic Tissue
 Bone Antibodies  >141 publications with aFGF and Bone
 Heart Antibodies  >118 publications with aFGF and Heart
 Liver Antibodies  >116 publications with aFGF and Liver
 Lung Antibodies  >112 publications with aFGF and Lung
 Eye Antibodies  >106 publications with aFGF and Eye
 Kidney Antibodies  >83 publications with aFGF and Kidney
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