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GAPDH elisa kit :: Goat Glyceraldehyde 3 Phosphate Dehydrogenase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS011154 GAPDH elisa kit
Unit / Price
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 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glyceraldehyde 3 Phosphate Dehydrogenase (GAPDH), ELISA Kit

 Also Known As   

Goat Glyceraldehyde 3 Phosphate Dehydrogenase ELISA Kit

 Product Gene Name   

GAPDH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 4; NC_005103.3 (224693580..224697455, complement). Location: 4q42
 3D Structure    ModBase 3D Structure for P04797
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 Species Reactivity    Goat
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 Assay Type    Sandwich
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GAPDH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GAPDH purchase    MBS011154 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glyceraldehyde 3 Phosphate Dehydrogenase (GAPDH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GAPDH. The ELISA analytical biochemical technique of the MBS011154 kit is based on GAPDH antibody-GAPDH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GAPDH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GAPDH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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NCBI/Uniprot data below describe general gene information for GAPDH. It may not necessarily be applicable to this product.
 NCBI GI #    122065190
 NCBI GeneID    24383
 NCBI Accession #    P04797.3 [Other Products]
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 UniProt Primary Accession #    P04797 [Other Products]
 UniProt Secondary Accession #    P09328; Q5M916; Q9QWU4 [Other Products]
 UniProt Related Accession #    P04797 [Other Products]
 Molecular Weight    35,828 Da
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 NCBI Official Full Name    Glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Synonym Full Names    glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Symbol    Gapdh [Similar Products]
 NCBI Official Synonym Symbols   
Gapd
[Similar Products]
 NCBI Protein Information    glyceraldehyde-3-phosphate dehydrogenase; BARS-38; peptidyl-cysteine S-nitrosylase GAPDH; 38 kDa BFA-dependent ADP-ribosylation substrate
 UniProt Protein Name    Glyceraldehyde-3-phosphate dehydrogenase
 UniProt Synonym Protein Names   
38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH
 UniProt Gene Name    Gapdh [Similar Products]
 UniProt Synonym Gene Names    Gapd; GAPDH [Similar Products]
 UniProt Entry Name    G3P_RAT
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 NCBI Summary for GAPDH    catalyzes the interconversion of D-glyceraldehyde 3-phosphate, phosphate, and NAD(+) to 3-phospho-D-glyceroyl phosphate and NADH; may contribute to apoptosis [RGD, Feb 2006]
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 UniProt Comments for GAPDH    GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma

Protein type: EC 1.2.1.12; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase

Cellular Component: microtubule cytoskeleton; nuclear membrane; membrane; mitochondrion; intracellular membrane-bound organelle; cytoplasm; plasma membrane; lipid particle; ribonucleoprotein complex; nucleus; cytosol; vesicle

Molecular Function: identical protein binding; protein binding; microtubule binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; NADP binding; NAD binding

Biological Process: regulation of translation; neuron apoptosis; glycolysis; protein stabilization; apoptosis; negative regulation of translation; multicellular organismal development; carbohydrate metabolic process; microtubule cytoskeleton organization and biogenesis; gluconeogenesis
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 Research Articles on GAPDH    1. Data show statistically significant altered levels of Tpi1 and alteration of Hk1, Aldoc, Pgam1 and Gapdh with borderline significance in phencyclidine (PCP) rats compared to controls.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GAPDH elisa kitPathways associated with GAPDH elisa kit
 Reference Product  PubMed Publications
 Actb elisa kit  >170 publications with GAPDH and Actb
 Egfr elisa kit  >9 publications with GAPDH and Egfr
 Alb elisa kit  >5 publications with GAPDH and Alb
 Akt1 elisa kit  >3 publications with GAPDH and Akt1
 Il2 elisa kit  >1 publications with GAPDH and Il2
 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
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 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
Diseases associated with GAPDH elisa kitOrgans/Tissues associated with GAPDH elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >578 publications with GAPDH and Neoplasms
 Nervous System Diseases Antibodies  >228 publications with GAPDH and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >210 publications with GAPDH and Cardiovascular Diseases
 Brain Diseases Antibodies  >175 publications with GAPDH and Brain Diseases
 Necrosis Antibodies  >158 publications with GAPDH and Necrosis
 Inflammation Antibodies  >141 publications with GAPDH and Inflammation
 Heart Diseases Antibodies  >125 publications with GAPDH and Heart Diseases
 Drug Toxicity Antibodies  >123 publications with GAPDH and Drug Toxicity
 Liver Diseases Antibodies  >106 publications with GAPDH and Liver Diseases
 Kidney Diseases Antibodies  >83 publications with GAPDH and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >470 publications with GAPDH and Muscle
 Brain Antibodies  >417 publications with GAPDH and Brain
 Liver Antibodies  >325 publications with GAPDH and Liver
 Heart Antibodies  >247 publications with GAPDH and Heart
 Kidney Antibodies  >212 publications with GAPDH and Kidney
 Lung Antibodies  >183 publications with GAPDH and Lung
 Embryonic Tissue Antibodies  >105 publications with GAPDH and Embryonic Tissue
 Eye Antibodies  >80 publications with GAPDH and Eye
 Connective Tissue Antibodies  >66 publications with GAPDH and Connective Tissue
 Colon Antibodies  >58 publications with GAPDH and Colon
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