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LH elisa kit :: Goat Luteinizing Hormone (LH) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703502
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
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 Product Name   

Luteinizing Hormone (LH), ELISA Kit

★Popular Item★
 Also Known As   

Goat Luteinizing Hormone, LH ELISA Kit

 Product Synonym Names    Goat Luteinizing Hormone (LH) ELISA KIT; Luteinizing Hormone (LH)
 Product Gene Name   

LH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703502 Testing Data    Testing Data PDF
 OMIM    238320
 3D Structure    ModBase 3D Structure for P22888
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 Species Reactivity    Goat
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    0.3 mlU/ml-60 mlU/ml
 Sensitivity    0.15 mlU/ml
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LH purchase    MBS703502 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Luteinizing Hormone (LH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.3 mlU/ml-60 mlU/ml in biological research samples containing LH, with an estimated sensitivity of 0.15 mlU/ml. The ELISA analytical biochemical technique of the MBS703502 kit is based on LH antibody-LH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LH elisa kit

   Introduction: Luteinizing hormone (LH, also known as lutropin) is a hormone produced by the anterior pituitary gland. LH is a glycoprotein. Each monomeric unit is a sugar-like protein molecule; two of these make the full, functional protein. Its structure is similar to the other glycoproteins, follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and human chorionic gonadotropin (hCG). The protein dimer contains 2 polypeptide units, labeled alpha and beta subunits that are connected by two disulfide bridges: The different composition of these oligosaccharides affects bioactivity and speed of degradation. The biologic half-life of LH is 20 minutes, shorter than that of FSH (3-4 hours) or hCG (24 hours). The gene for the alpha subunit is located on chromosome 6q12.21. The LH beta subunit gene is localized in the LHB/CGB gene cluster on chromosome 19q13.32. In contrast to the alpha gene activity, beta LH subunit gene activity is restricted to the pituitary gonadotropic cells. It is regulated by the gonadotropin releasing hormone from the hypothalamus. Inhibin, activin, and sex hormones do not affect genetic activity for the beta subunit 3 production of LH. LH levels are normally low during childhood and, in women, high after menopause. During the reproductive years typical levels are between 5-20 mIU/ml. In females, at the time of menstruation, FSH initiates follicular growth, specifically affecting granulosa cells. With the rise in estrogens, LH receptors are also expressed on the maturing follicle that produces an increasing amount of estradiol. Eventually at the time of the maturation of the follicle, the estrogen rise leads via the hypothalamic interface to the "positive feed-back" effect, a release of LH over a 24-48 hour period. This 'LH surge' triggers ovulation hereby not only releasing the egg, but also initiating the conversion of the residual follicle into a corpus luteum that, in turn, produces progesterone to prepare the endometrium for a possible implantation. LH is necessary to maintain luteal function for the first two weeks. In case of a pregnancy luteal function will be further maintained by the action of hCG (a hormone very similar to LH) from the newly established pregnancy. LH supports thecal cells in the ovary that provide androgens and hormonal precursors for estradiol production. In the male, LH acts upon the Leydig cells of the testis and is responsible for the production of testosterone, an androgen that exerts both endocrine activity and intratesticular 4 activity such as spermatogenesis. The release of LH at the pituitary gland is controlled by pulses of gonadotropin-releasing hormone (GnRH) from the hypothalamus. Those pulses, in turn, are subject to the estrogen feedback from the gonads.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with a goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated LH and anti-LH antibody and incubated. Then substrate solution A and B are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of LH elisa kit    LH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703502. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LH. It may not necessarily be applicable to this product.
 NCBI GI #    7704943
 NCBI GeneID    3973
 NCBI Accession #    AAB19917.2 [Other Products]
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 UniProt Primary Accession #    P22888 [Other Products]
 UniProt Secondary Accession #    Q14751; Q15996; Q9UEW9 [Other Products]
 UniProt Related Accession #    P22888 [Other Products]
 Molecular Weight    71,615 Da
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 NCBI Official Full Name    luteinizing hormone receptor
 NCBI Official Synonym Full Names    luteinizing hormone/choriogonadotropin receptor
 NCBI Official Symbol    LHCGR [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    lutropin-choriogonadotropic hormone receptor; hypergonadotropic hypogonadism; lutropin/choriogonadotropin receptor
 UniProt Protein Name    Lutropin-choriogonadotropic hormone receptor
 UniProt Synonym Protein Names   
Luteinizing hormone receptor; LHR; LSH-R
 UniProt Gene Name    LHCGR [Similar Products]
 UniProt Synonym Gene Names    LCGR; LGR2; LHRHR; LH/CG-R; LHR; LSH-R [Similar Products]
 UniProt Entry Name    LSHR_HUMAN
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 NCBI Summary for LH    This gene encodes the receptor for both luteinizing hormone and choriogonadotropin. This receptor belongs to the G-protein coupled receptor 1 family, and its activity is mediated by G proteins which activate adenylate cyclase. Mutations in this gene result in disorders of male secondary sexual character development, including familial male precocious puberty, also known as testotoxicosis, hypogonadotropic hypogonadism, Leydig cell adenoma with precocious puberty, and male pseudohermaphtoditism with Leydig cell hypoplasia. [provided by RefSeq, Jul 2008]
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 UniProt Comments for LH    Function: Receptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
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 Research Articles on LH    1. results of the experiments showed that hCGbeta action leads to the increase in ERK and AKT kinases phosphorylation in cancer cells and indicate that these biological effects can be achieved independently of human chorionic gonadotropin presence
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LH elisa kitPathways associated with LH elisa kit
 Reference Product  PubMed Publications
 LHB elisa kit  >10 publications with LH and LHB
 TSHR elisa kit  >5 publications with LH and TSHR
 GIPR elisa kit  >2 publications with LH and GIPR
 CGA elisa kit  >2 publications with LH and CGA
 POMC elisa kit  >1 publications with LH and POMC
 GNAS elisa kit  >1 publications with LH and GNAS
 Products by Pathway  Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Hormone Ligand-binding Receptors Pathway antibodies  Hormone Ligand-binding Receptors Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
Diseases associated with LH elisa kitOrgans/Tissues associated with LH elisa kit
 Disease Name  Pubmed Publications
 Ovarian Diseases Antibodies  >24 publications with LH and Ovarian Diseases
 Infertility, Female Antibodies  >18 publications with LH and Infertility, Female
 Puberty, Precocious Antibodies  >11 publications with LH and Puberty, Precocious
 Infertility, Male Antibodies  >9 publications with LH and Infertility, Male
 Urogenital Abnormalities Antibodies  >8 publications with LH and Urogenital Abnormalities
 Leydig Cell Hypoplasia Antibodies  >7 publications with LH and Leydig Cell Hypoplasia
 Ovarian Neoplasms Antibodies  >7 publications with LH and Ovarian Neoplasms
 Disorders of Sex Development Antibodies  >7 publications with LH and Disorders of Sex Development
 Genital Diseases, Male Antibodies  >6 publications with LH and Genital Diseases, Male
 Hyperplasia Antibodies  >5 publications with LH and Hyperplasia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >19 publications with LH and Blood
 Testis Antibodies  >16 publications with LH and Testis
 Uterus Antibodies  >5 publications with LH and Uterus
 Bone Antibodies  >4 publications with LH and Bone
 Brain Antibodies  >4 publications with LH and Brain
 Prostate Antibodies  >1 publications with LH and Prostate
 Bladder Antibodies  >1 publications with LH and Bladder
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