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Slit2 elisa kit :: Goat Slit Homolog 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS739064
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Slit Homolog 2 (Slit2), ELISA Kit

 Also Known As   

Goat Slit Homolog 2 ELISA Kit

 Product Gene Name   

Slit2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 4; NC_000004.11 (20255235..20620788). Location: 4p15.2
 OMIM    603746
 3D Structure    ModBase 3D Structure for O94813
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 Species Reactivity    Goat
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
 Intended Uses    This Slit2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat Slit2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Slit2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Slit2 purchase    MBS739064 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Slit Homolog 2 (Slit2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Slit2, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS739064 kit is based on Slit2 antibody-Slit2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Slit2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Slit2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for Slit2 elisa kit

   Principle of the Assay: Slit2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Slit2 antibody and an Slit2-HRP conjugate. The assay sample and buffer are incubated together with Slit2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Slit2 concentration since Slit2 from samples and Slit2-HRP conjugate compete for the anti-Slit2 antibody binding site. Since the number of sites is limited, as more sites are occupied by Slit2 from the sample, fewer sites are left to bind Slit2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Slit2 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for Slit2 elisa kit    Neurobiology
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 Typical Testing Data/Standard Curve (for reference only) of Slit2 elisa kit    Slit2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS739064. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Slit2. It may not necessarily be applicable to this product.
 NCBI GI #    4759146
 NCBI GeneID    9353
 NCBI Accession #    NP_004778.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004787.1 [Other Products]
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 UniProt Primary Accession #    O94813 [Other Products]
 UniProt Secondary Accession #    O95710; Q17RU3; Q9Y5Q7; B7ZLR5 [Other Products]
 UniProt Related Accession #    O94813; Q4W5K2; Q4W5N0 [Other Products]
 Molecular Weight    169,870 Da
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 NCBI Official Full Name    slit homolog 2 protein
 NCBI Official Synonym Full Names    slit homolog 2 (Drosophila)
 NCBI Official Symbol    SLIT2 [Similar Products]
 NCBI Official Synonym Symbols   
SLIL3; Slit-2
[Similar Products]
 NCBI Protein Information    slit homolog 2 protein
 UniProt Protein Name    Slit homolog 2 protein
 Protein Family    Slit homolog 2 protein
 UniProt Gene Name    SLIT2 [Similar Products]
 UniProt Synonym Gene Names    SLIL3; Slit-2 [Similar Products]
 UniProt Entry Name    SLIT2_HUMAN
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 NCBI Summary for Slit2    This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
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 UniProt Comments for Slit2    SLIT2: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth- stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post- crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: extracellular space; cell surface; membrane; cytoplasm; extracellular region; plasma membrane

Molecular Function: laminin-1 binding; heparin binding; identical protein binding; proteoglycan binding; protein binding; protein homodimerization activity; Roundabout binding; chemorepellent activity; calcium ion binding; GTPase inhibitor activity

Biological Process: negative regulation of small GTPase mediated signal transduction; axon guidance; positive regulation of apoptosis; negative chemotaxis; motor axon guidance; negative regulation of axon extension; negative regulation of smooth muscle cell migration; induction of negative chemotaxis; corticospinal neuron axon guidance through the spinal cord; negative regulation of cell proliferation; cell-cell adhesion; response to cortisol stimulus; negative regulation of protein amino acid phosphorylation; ureteric bud development; negative regulation of leukocyte chemotaxis; negative regulation of cell migration; negative regulation of actin filament polymerization; in utero embryonic development; chemorepulsion involved in postnatal olfactory bulb interneuron migration; dorsal/ventral axon guidance; cellular response to hormone stimulus; axon extension involved in axon guidance; chemorepulsion involved in embryonic olfactory bulb interneuron migration; cell migration during sprouting angiogenesis; branching morphogenesis of a tube; negative regulation of catalytic activity; negative regulation of vascular permeability; positive regulation of axonogenesis; negative regulation of cell growth; metanephros development; retinal ganglion cell axon guidance
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 Research Articles on Slit2    1. targeting Slit2/Robo4 signaling may protect the integrity of the lymphatic barrier and limit the dissemination of HIV in the host.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Slit2 elisa kitPathways associated with Slit2 elisa kit
 Reference Product  PubMed Publications
 ROBO1 elisa kit  >79 publications with Slit2 and ROBO1
 ROBO2 elisa kit  >54 publications with Slit2 and ROBO2
 Products by Pathway  Pathway Diagram
 Activation Of Rac Pathway antibodies  Activation Of Rac Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Glypican 1 Network Pathway antibodies  Glypican 1 Network Pathway Diagram
 Inactivation Of Cdc42 And Rac Pathway antibodies  Inactivation Of Cdc42 And Rac Pathway Diagram
 Inactivation Of Cdc42 And Rac Pathway antibodies  Inactivation Of Cdc42 And Rac Pathway Diagram
Diseases associated with Slit2 elisa kitOrgans/Tissues associated with Slit2 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >53 publications with Slit2 and Neoplasms
 Carcinoma Antibodies  >31 publications with Slit2 and Carcinoma
 Nervous System Diseases Antibodies  >19 publications with Slit2 and Nervous System Diseases
 Breast Neoplasms Antibodies  >18 publications with Slit2 and Breast Neoplasms
 Lung Neoplasms Antibodies  >13 publications with Slit2 and Lung Neoplasms
 Skin Diseases Antibodies  >10 publications with Slit2 and Skin Diseases
 Brain Diseases Antibodies  >9 publications with Slit2 and Brain Diseases
 Neovascularization, Pathologic Antibodies  >8 publications with Slit2 and Neovascularization, Pathologic
 Carcinoma, Squamous Cell Antibodies  >7 publications with Slit2 and Carcinoma, Squamous Cell
 Disease Models, Animal Antibodies  >6 publications with Slit2 and Disease Models, Animal
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >72 publications with Slit2 and Brain
 Embryonic Tissue Antibodies  >51 publications with Slit2 and Embryonic Tissue
 Eye Antibodies  >28 publications with Slit2 and Eye
 Vascular Antibodies  >24 publications with Slit2 and Vascular
 Lung Antibodies  >17 publications with Slit2 and Lung
 Kidney Antibodies  >14 publications with Slit2 and Kidney
 Ganglia Antibodies  >10 publications with Slit2 and Ganglia
 Muscle Antibodies  >6 publications with Slit2 and Muscle
 Liver Antibodies  >5 publications with Slit2 and Liver
 Prostate Antibodies  >5 publications with Slit2 and Prostate
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