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TNF elisa kit :: Goat tumor necrosis factor (TNF superfamily, member 2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704211
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

tumor necrosis factor (TNF superfamily, member 2), ELISA Kit

★Popular Item★
 Also Known As   

Goat Tumor necrosis factor alpha, TNF-alpha ELISA Kit

 Product Synonym Names    Goat Tumor necrosis factor alpha; TNF-alpha ELISA KIT; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily; member 2)
 Product Gene Name   

TNF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704211 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P13296
 Species Reactivity    Goat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Goat TNF. No significant cross-reactivity or interference between Goat TNF and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    15.6 pg/ml-1000 pg/ml
 Sensitivity    3.9 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TNF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TNF purchase    MBS704211 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tumor necrosis factor (TNF superfamily, member 2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml-1000 pg/ml in biological research samples containing TNF, with an estimated sensitivity of 3.9 pg/ml. The ELISA analytical biochemical technique of the MBS704211 kit is based on TNF antibody-TNF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TNF elisa kit

   Introduction: Tumor necrosis factor (TNF, cachexin or cachectin and formally known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction.The primary role of TNF is in the regulation of immune cells. TNF is also able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases, as well as cancer.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TNF-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TNF-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TNF-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TNF elisa kit    TNF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704211. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TNF. It may not necessarily be applicable to this product.
 NCBI GI #    556695474
 NCBI GeneID    100861232
 NCBI Accession #    NP_001273371.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001286442.1 [Other Products]
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 UniProt Primary Accession #    P13296 [Other Products]
 UniProt Secondary Accession #    Q28320; Q9MYZ2 [Other Products]
 UniProt Related Accession #    P13296 [Other Products]
 Molecular Weight    25,519 Da
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 NCBI Official Full Name    tumor necrosis factor
 NCBI Official Symbol    TNF [Similar Products]
 NCBI Official Synonym Symbols   
TNFA; TNF-a; TNFSF2; Cachectin; TNF-alpha
[Similar Products]
 NCBI Protein Information    tumor necrosis factor; tumor necrosis factor ligand superfamily member 2
 UniProt Protein Name    Tumor necrosis factor
 UniProt Synonym Protein Names   
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
 Protein Family    Tumor necrosis factor
 UniProt Gene Name    TNF [Similar Products]
 UniProt Synonym Gene Names    TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2 [Similar Products]
 UniProt Entry Name    TNFA_CAPHI
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 UniProt Comments for TNF    Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TNF elisa kitPathways associated with TNF elisa kit
 Reference Product  PubMed Publications
 TRAF2 elisa kit  >864 publications with TNF and TRAF2
 TNFRSF1A elisa kit  >298 publications with TNF and TNFRSF1A
 IFNG elisa kit  >92 publications with TNF and IFNG
 ADAM17 elisa kit  >91 publications with TNF and ADAM17
 TNFRSF1B elisa kit  >75 publications with TNF and TNFRSF1B
 PRTN3 elisa kit  >42 publications with TNF and PRTN3
 DCN elisa kit  >12 publications with TNF and DCN
 CSF1 elisa kit  >12 publications with TNF and CSF1
 ADAM9 elisa kit  >8 publications with TNF and ADAM9
 BGN elisa kit  >6 publications with TNF and BGN
 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
Diseases associated with TNF elisa kitOrgans/Tissues associated with TNF elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >87971 publications with TNF and Necrosis
 Inflammation Antibodies  >31113 publications with TNF and Inflammation
 Vascular Diseases Antibodies  >7249 publications with TNF and Vascular Diseases
 Lung Diseases Antibodies  >6502 publications with TNF and Lung Diseases
 Liver Diseases Antibodies  >4918 publications with TNF and Liver Diseases
 Insulin Resistance Antibodies  >2393 publications with TNF and Insulin Resistance
 Fibrosis Antibodies  >2375 publications with TNF and Fibrosis
 Atherosclerosis Antibodies  >2305 publications with TNF and Atherosclerosis
 Colitis Antibodies  >2212 publications with TNF and Colitis
 Pain Antibodies  >2140 publications with TNF and Pain
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >42516 publications with TNF and Blood
 Lung Antibodies  >7682 publications with TNF and Lung
 Brain Antibodies  >5127 publications with TNF and Brain
 Heart Antibodies  >3863 publications with TNF and Heart
 Intestine Antibodies  >2807 publications with TNF and Intestine
 Connective Tissue Antibodies  >1770 publications with TNF and Connective Tissue
 Lymph Node Antibodies  >1653 publications with TNF and Lymph Node
 Placenta Antibodies  >698 publications with TNF and Placenta
 Prostate Antibodies  >648 publications with TNF and Prostate
 Ovary Antibodies  >468 publications with TNF and Ovary
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