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BDG elisa kit :: Guinea Pig 1, 3-beta-D-glucan ELISA Kit

Scan QR to view Datasheet Catalog #    MBS726966
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

1, 3-beta-D-glucan (BDG), ELISA Kit

 Also Known As   

Guinea pig 1, 3-beta-D-glucan ELISA Kit

 Product Synonym Names    Guinea pig 1, 3-b-D-glucan ELISA Kit
 Product Gene Name   

BDG elisa kit

[Similar Products]
 Product Synonym Gene Name    BDG [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Guinea Pig
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 Samples    Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BDG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BDG purchase    MBS726966 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1, 3-beta-D-glucan (BDG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BDG. The ELISA analytical biochemical technique of the MBS726966 kit is based on BDG antibody-BDG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BDG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BDG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for BDG elisa kit

   For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma

Intended Uses: This B2M ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of B2M in the sample, this B2M ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus B2M concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of B2M utilizes a multiclonal anti-B2M antibody and an B2M-HRP conjugate. The assay sample and buffer are incubated together with B2M-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the B2M concentration since B2M from samples and B2M-HRP conjugate compete for the anti-B2M antibody binding site. Since the number of sites is limited, as more sites are occupied by B2M from the sample, fewer sites are left to bind B2M-HRP conjugate. Standards of known B2M concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of B2M. The B2M concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of BDG elisa kit    BDG elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS726966. Click to request current manual
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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