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HSPG elisa kit :: Guinea Pig Heparan Sulphate Proteoglycan ELISA Kit

Scan QR to view Datasheet Catalog #    MBS732716
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Heparan Sulphate Proteoglycan (HSPG), ELISA Kit

★Popular Item★
 Also Known As   

Guinea pig Heparan Sulphate Proteoglycan ELISA Kit

 Product Gene Name   

HSPG elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    255800
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 Species Reactivity    Guinea Pig
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 Specificity    This assay has high sensitivity and excellent specificity for detection of HSPG. No significant cross-reactivity or interference between HSPG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSPG and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This HSPG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig HSPG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HSPG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HSPG purchase    MBS732716 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Heparan Sulphate Proteoglycan (HSPG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing HSPG, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS732716 kit is based on HSPG antibody-HSPG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSPG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSPG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HSPG elisa kit

   Principle of the assay: HSPG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSPG antibody and an HSPG-HRP conjugate. The assay sample and buffer are incubated together with HSPG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSPG concentration since HSPG from samples and HSPG-HRP conjugate compete for the anti-HSPG antibody binding site. Since the number of sites is limited, as more sites are occupied by HSPG from the sample, fewer sites are left to bind HSPG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSPG concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for HSPG elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of HSPG elisa kit    HSPG elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS732716. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HSPG. It may not necessarily be applicable to this product.
 NCBI GI #    13242858
 NCBI GeneID    3339
 NCBI Accession #    AAB21121.2 [Other Products]
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 UniProt Secondary Accession #    Q16287; Q5SZI3; Q9H3V5 [Other Products]
 UniProt Related Accession #    P98160 [Other Products]
 Molecular Weight    468,830 Da
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 NCBI Official Full Name    heparan sulfate proteoglycan core protein, partial
 NCBI Official Synonym Full Names    heparan sulfate proteoglycan 2
 NCBI Official Symbol    HSPG2 [Similar Products]
 NCBI Official Synonym Symbols   
PLC; SJA; SJS; HSPG; SJS1; PRCAN
[Similar Products]
 NCBI Protein Information    basement membrane-specific heparan sulfate proteoglycan core protein; perlecan proteoglycan; endorepellin (domain V region)
 UniProt Protein Name    Basement membrane-specific heparan sulfate proteoglycan core protein
 UniProt Synonym Protein Names   
Perlecan; PLC
 UniProt Gene Name    HSPG2 [Similar Products]
 UniProt Synonym Gene Names    HSPG; PLC [Similar Products]
 UniProt Entry Name    PGBM_HUMAN
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 NCBI Summary for HSPG    This gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and transthyretin, etc., and it plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and tardive dyskinesia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]
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 UniProt Comments for HSPG    HSPG2: Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development. Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1); a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses. Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH). The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.

Protein type: Motility/polarity/chemotaxis; Secreted; Cell adhesion; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.1-p34

Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; focal adhesion; Golgi lumen; plasma membrane; extracellular region; basal lamina

Molecular Function: protein C-terminus binding; protein binding; metal ion binding

Biological Process: cardiac muscle development; phototransduction, visible light; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; pathogenesis; lipoprotein metabolic process; embryonic skeletal morphogenesis; chondroitin sulfate metabolic process; extracellular matrix disassembly; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; protein localization; carbohydrate metabolic process; chondrocyte differentiation; angiogenesis; brain development; retinoid metabolic process; endochondral ossification

Disease: Schwartz-jampel Syndrome, Type 1; Dyssegmental Dysplasia, Silverman-handmaker Type
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 Research Articles on HSPG    1. role for perlecan in chondrogenic and osteogenic events which drive discal development and ossification of the vertebral bodies.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HSPG elisa kitPathways associated with HSPG elisa kit
 Reference Product  PubMed Publications
 SDC2 elisa kit  >2 publications with HSPG and SDC2
 HPSE elisa kit  >1 publications with HSPG and HPSE
 BGN elisa kit  >1 publications with HSPG and BGN
 APP elisa kit  >1 publications with HSPG and APP
 Products by Pathway  Pathway Diagram
 A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway antibodies  A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies  Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
Diseases associated with HSPG elisa kitOrgans/Tissues associated with HSPG elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >17 publications with HSPG and Nervous System Diseases
 Mental Disorders Antibodies  >9 publications with HSPG and Mental Disorders
 Osteochondrodysplasias Antibodies  >9 publications with HSPG and Osteochondrodysplasias
 Disease Models, Animal Antibodies  >6 publications with HSPG and Disease Models, Animal
 Dyssegmental dysplasia Antibodies  >6 publications with HSPG and Dyssegmental dysplasia
 Kidney Diseases Antibodies  >5 publications with HSPG and Kidney Diseases
 Dementia Antibodies  >5 publications with HSPG and Dementia
 Alzheimer Disease Antibodies  >5 publications with HSPG and Alzheimer Disease
 Movement Disorders Antibodies  >5 publications with HSPG and Movement Disorders
 Congenital Abnormalities Antibodies  >4 publications with HSPG and Congenital Abnormalities
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >14 publications with HSPG and Bone
 Blood Antibodies  >12 publications with HSPG and Blood
 Vascular Antibodies  >10 publications with HSPG and Vascular
 Muscle Antibodies  >9 publications with HSPG and Muscle
 Connective Tissue Antibodies  >7 publications with HSPG and Connective Tissue
 Embryonic Tissue Antibodies  >6 publications with HSPG and Embryonic Tissue
 Ovary Antibodies  >5 publications with HSPG and Ovary
 Kidney Antibodies  >5 publications with HSPG and Kidney
 Eye Antibodies  >4 publications with HSPG and Eye
 Brain Antibodies  >4 publications with HSPG and Brain
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