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HNF1beta elisa kit :: Guinea Pig Hepatocyte Nuclear Factor 1 Beta ELISA Kit

Scan QR to view Datasheet Catalog #    MBS730787
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hepatocyte Nuclear Factor 1 Beta (HNF1beta), ELISA Kit

 Also Known As   

Guinea pig Hepatocyte Nuclear Factor 1 Beta ELISA Kit

 Product Gene Name   

HNF1beta elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    gene 611955
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 Species Reactivity    Guinea Pig
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 Assay Type    Sandwich
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact technical support.
 Other Notes    Small volumes of HNF1beta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HNF1beta purchase    MBS730787 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hepatocyte Nuclear Factor 1 Beta (HNF1beta) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards render a theoretical kit detection range in biological research samples containing HNF1beta. The ELISA analytical biochemical technique of the MBS730787 kit is based on HNF1beta antibody-HNF1beta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HNF1beta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HNF1beta. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for HNF1beta elisa kit

   Intended Uses: This HNF1b ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHNF1b in the sample, thisHNF1b ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHNF1b concentration. The concentration ofHNF1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This HNF1b enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHNF1b. Standards or samples are then added to the microtiter plate wells andHNF1b if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHNF1b present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHNF1b are added to each well to "sandwich" theHNF1b immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containHNF1b and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
 Product Categories/Family for HNF1beta elisa kit    Epigenetics and Nuclear Signaling
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 Typical Testing Data/Standard Curve (for reference only) of HNF1beta elisa kit    HNF1beta elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS730787
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NCBI/Uniprot data below describe general gene information for HNF1beta. It may not necessarily be applicable to this product.
 NCBI GI #    260064065
 NCBI GeneID    6928
 NCBI Accession #    NP_001159395.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001165923.2 [Other Products]
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 UniProt Secondary Accession #    B4DKM3; E0YMJ9 [Other Products]
 UniProt Related Accession #    P35680 [Other Products]
 Molecular Weight    46,907 Da
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 NCBI Official Full Name    hepatocyte nuclear factor 1-beta isoform 2
 NCBI Official Synonym Full Names    HNF1 homeobox B
 NCBI Official Symbol    HNF1B [Similar Products]
 NCBI Official Synonym Symbols   
FJHN; HNF2; LFB3; TCF2; HPC11; LF-B3; MODY5; TCF-2; VHNF1; HNF-1B; HNF1beta
[Similar Products]
 NCBI Protein Information    hepatocyte nuclear factor 1-beta; HNF-1-beta; HNF1 beta A; homeoprotein LFB3; transcription factor 2, hepatic
 UniProt Protein Name    Hepatocyte nuclear factor 1-beta
 UniProt Synonym Protein Names   
Homeoprotein LFB3; Transcription factor 2; TCF-2; Variant hepatic nuclear factor 1; vHNF1
 UniProt Gene Name    HNF1B [Similar Products]
 UniProt Synonym Gene Names    TCF2; HNF-1-beta; HNF-1B; TCF-2; vHNF1 [Similar Products]
 UniProt Entry Name    HNF1B_HUMAN
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 NCBI Summary for HNF1beta    This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
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 UniProt Comments for HNF1beta    TCF2: Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in HNF1B are the cause of renal cysts and diabetes syndrome (RCAD); also called maturity-onset diabetes of the young type 5 (MODY5) or familial hypoplastic glomerulocystic kidney disease (GCKD). RCAD is an autosomal dominant disorder comprising non-diabetic renal disease resulting from abnormal renal development, and diabetes, which in some cases occurs earlier than age 25 years and is thus consistent with a diagnosis of maturity-onset diabetes of the young (MODY5). The renal disease is highly variable and includes renal cysts, glomerular tufts, aberrant nephrogenesis, primitive tubules, irregular collecting systems, oligomeganephronia, enlarged renal pelves, abnormal calyces, small kidney, single kidney, horseshoe kidney, and hyperuricemic nephropathy. Defects in HNF1B may be rare genetic risk factor contributing to the development of non-insulin-dependent diabetes mellitus (NIDDM). NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. Defects in HNF1B may be a cause of susceptibility to prostate cancer hereditary type 11 (HPC11). It is a condition associated with familial predisposition to cancer of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Belongs to the HNF1 homeobox family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; endodermal cell fate specification; regulation of Wnt receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; endocrine pancreas development; pronephros development; anterior/posterior pattern formation; epithelial cell proliferation; genitalia development; inner cell mass cell differentiation; branching morphogenesis of a tube; embryonic digestive tract morphogenesis; insulin secretion; response to glucose stimulus; regulation of endodermal cell fate specification; kidney development; hindbrain development

Disease: Renal Cysts And Diabetes Syndrome; Renal Cell Carcinoma, Nonpapillary; Diabetes Mellitus, Noninsulin-dependent
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 Research Articles on HNF1beta    1. While HNF1B disease was still unknown one decade ago, it has emerged as the second most prevalent dominantly inherited kidney disease. Data available pave the way for early recognition and improved specific management, including genetic counselling.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Guinea pig Hepatocyte Nuclear Factor 1 Beta ELISA Kit
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Products associated with HNF1beta elisa kitPathways associated with HNF1beta elisa kit
 Reference Product  PubMed Publications
 PKHD1 elisa kit  >12 publications with HNF1beta and PKHD1
 UMOD elisa kit  >10 publications with HNF1beta and UMOD
 PTF1A elisa kit  >7 publications with HNF1beta and PTF1A
 SOX9 elisa kit  >6 publications with HNF1beta and SOX9
 FXYD2 elisa kit  >5 publications with HNF1beta and FXYD2
 DPP4 elisa kit  >1 publications with HNF1beta and DPP4
 ATF1 elisa kit  >1 publications with HNF1beta and ATF1
 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram
 Regulation Of Beta-cell Development Pathway antibodies  Regulation Of Beta-cell Development Pathway Diagram
 Regulation Of Gene Expression In Early Pancreatic Precursor Cells Pathway antibodies  Regulation Of Gene Expression In Early Pancreatic Precursor Cells Pathway Diagram
 Regulation Of Gene Expression In Late Stage (branching Morphogenesis) Pancreatic Bud Precursor Cells Pathway antibodies  Regulation Of Gene Expression In Late Stage (branching Morphogenesis) Pancreatic Bud Precursor Cells Pathway Diagram
Diseases associated with HNF1beta elisa kitOrgans/Tissues associated with HNF1beta elisa kit
 Disease Name  Pubmed Publications
 Diabetes Mellitus, Type 2 Antibodies  >255 publications with HNF1beta and Diabetes Mellitus, Type 2
 Kidney Diseases Antibodies  >112 publications with HNF1beta and Kidney Diseases
 Liver Diseases Antibodies  >94 publications with HNF1beta and Liver Diseases
 Liver Neoplasms Antibodies  >66 publications with HNF1beta and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >42 publications with HNF1beta and Carcinoma, Hepatocellular
 Renal Insufficiency Antibodies  >22 publications with HNF1beta and Renal Insufficiency
 Renal cysts and diabetes syndrome Antibodies  >19 publications with HNF1beta and Renal cysts and diabetes syndrome
 Ovarian Neoplasms Antibodies  >18 publications with HNF1beta and Ovarian Neoplasms
 Prostatic Neoplasms Antibodies  >17 publications with HNF1beta and Prostatic Neoplasms
 Nervous System Diseases Antibodies  >16 publications with HNF1beta and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >275 publications with HNF1beta and Liver
 Kidney Antibodies  >185 publications with HNF1beta and Kidney
 Pancreas Antibodies  >122 publications with HNF1beta and Pancreas
 Blood Antibodies  >109 publications with HNF1beta and Blood
 Intestine Antibodies  >35 publications with HNF1beta and Intestine
 Prostate Antibodies  >24 publications with HNF1beta and Prostate
 Uterus Antibodies  >14 publications with HNF1beta and Uterus
 Brain Antibodies  >11 publications with HNF1beta and Brain
 Lung Antibodies  >9 publications with HNF1beta and Lung
 Testis Antibodies  >5 publications with HNF1beta and Testis
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