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IFN-alpha elisa kit :: Guinea Pig Interferon alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS731511
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $470 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $470 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $675 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,850 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,850 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,340 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,340 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interferon alpha (IFN-alpha), ELISA Kit

★Popular Item★
 Also Known As   

Guinea pig Interferon alpha ELISA Kit

 Product Synonym Names    Guinea pig Interferon a ELISA Kit
 Product Gene Name   

IFN-alpha elisa kit

[Similar Products]
 Product Synonym Gene Name    IFN-a [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    147568
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 Species Reactivity    Guinea Pig
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IFN?. No significant cross-reactivity or interference between IFN? and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IFN? and all the analogues, therefore, cross reaction may still exist in some cases.
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 Assay Type    Competitive or Sandwich
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Sensitivity    1.0 pg/mL.
 Intended Uses    This IFN? ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig IFN?. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IFN-alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IFN-alpha purchase    MBS731511 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interferon alpha (IFN-alpha) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFN-alpha, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS731511 kit is based on IFN-alpha antibody-IFN-alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFN-alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFN-alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IFN-alpha elisa kit

   Principle of the assay: IFN? ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IFN?. Standards or samples are then added to the microtiter plate wells and IFN? if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IFN? present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IFN? are added to each well to “sandwich” the IFN? immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFN? and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFN? concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for IFN-alpha elisa kit    Cancer
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 Typical Testing Data/Standard Curve (for reference only) of IFN-alpha elisa kit    IFN-alpha elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS731511. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IFN-alpha. It may not necessarily be applicable to this product.
 NCBI GI #    306902
 NCBI GeneID    3445
 NCBI Accession #    AAA52716.1 [Other Products]
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 UniProt Secondary Accession #    P01565; P09236; Q5VWV7; Q5VYQ3 [Other Products]
 UniProt Related Accession #    P32881 [Other Products]
 Molecular Weight    21,989 Da
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 NCBI Official Full Name    interferon-alpha
 NCBI Official Synonym Full Names    interferon, alpha 8
 NCBI Official Symbol    IFNA8 [Similar Products]
 NCBI Official Synonym Symbols   
IFN-alphaB
[Similar Products]
 NCBI Protein Information    interferon alpha-8; leIF B; IFN-alpha-8; interferon alpha-B; interferon alpha-B'; interferon alpha-B2; interferon alpha type 201
 UniProt Protein Name    Interferon alpha-8
 UniProt Synonym Protein Names   
Interferon alpha-B; LeIF B; Interferon alpha-B2
 Protein Family    Interferon
 UniProt Gene Name    IFNA8 [Similar Products]
 UniProt Synonym Gene Names    IFN-alpha-8; LeIF B [Similar Products]
 UniProt Entry Name    IFNA8_HUMAN
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 UniProt Comments for IFN-alpha    IFNA8: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Belongs to the alpha/beta interferon family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 9p22

Cellular Component: extracellular space; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; B cell differentiation; cytokine and chemokine mediated signaling pathway; natural killer cell activation during immune response; regulation of MHC class I biosynthetic process; innate immune response; blood coagulation; defense response to virus; humoral immune response; T cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein
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 Research Articles on IFN-alpha    1. IFN-alpha8 and -alpha10 most potently enhanced expression of IFN-gamma, IL-2, and IL-4.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IFN-alpha elisa kitPathways associated with IFN-alpha elisa kit
 Reference Product  PubMed Publications
 IFNA2 elisa kit  >13 publications with IFN-alpha and IFNA2
 IFNA6 elisa kit  >1 publications with IFN-alpha and IFNA6
 IFNA5 elisa kit  >1 publications with IFN-alpha and IFNA5
 IFNAR1 elisa kit  >1 publications with IFN-alpha and IFNAR1
 Products by Pathway  Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies  Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Hepatitis B Pathway antibodies  Hepatitis B Pathway Diagram
Diseases associated with IFN-alpha elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2 publications with IFN-alpha and Inflammation
 Nervous System Diseases Antibodies  >2 publications with IFN-alpha and Nervous System Diseases
 Leukemia Antibodies  >1 publications with IFN-alpha and Leukemia
 Leukemia, Myelogenous, Chronic, BCR-ABL Positive Antibodies  >1 publications with IFN-alpha and Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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