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PRG4 elisa kit :: Guinea Pig PRG4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2509591
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   


★Popular Item★
 Also Known As   

Guinea pig PRG4 (Proteoglycan 4) ELISA Kit

 Product Gene Name   

PRG4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    208250
 3D Structure    ModBase 3D Structure for Q92954
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 Species Reactivity    Guinea Pig
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 Specificity    This kit recognizes natural and recombinant Guinea pig PRG4. No significant cross-reactivity or interference between Guinea pig PRG4 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    1.563-100ng/mL
 Sensitivity    Min: 0.938ng/mL; Max: 100ng/mL
 Intended Uses    This ELISA kit applies to the in vitro quantitative determination of Guinea pig PRG4 concentrations in serum, plasma and other biological fluids.
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PRG4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PRG4 purchase    MBS2509591 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PRG4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.563-100ng/mL in biological research samples containing PRG4, with an estimated sensitivity of Min: 0.938ng/mL; Max: 100ng/mL. The ELISA analytical biochemical technique of the MBS2509591 kit is based on PRG4 antibody-PRG4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRG4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRG4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PRG4 elisa kit

   Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PRG4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PRG4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PRG4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PRG4. You can calculate the concentration of PRG4 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PRG4 elisa kit    PRG4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2509591. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PRG4. It may not necessarily be applicable to this product.
 NCBI GI #    67190163
 NCBI GeneID    10216
 NCBI Accession #    NP_005798.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_005807.3 [Other Products]
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 UniProt Primary Accession #    Q92954 [Other Products]
 UniProt Secondary Accession #    Q6DNC4; Q6DNC5; Q6ZMZ5; Q9BX49 [Other Products]
 UniProt Related Accession #    Q92954 [Other Products]
 Molecular Weight    146,468 Da
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 NCBI Official Full Name    proteoglycan 4 isoform A
 NCBI Official Synonym Full Names    proteoglycan 4
 NCBI Official Symbol    PRG4 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    proteoglycan 4; articular superficial zone protein; hemangiopoietin; lubricin; megakaryocyte stimulating factor; superficial zone proteoglycan
 UniProt Protein Name    Proteoglycan 4
 UniProt Synonym Protein Names   
Lubricin; Megakaryocyte-stimulating factor; Superficial zone proteoglycanProteoglycan 4 C-terminal part
 Protein Family    Proteoglycan
 UniProt Gene Name    PRG4 [Similar Products]
 UniProt Synonym Gene Names    MSF; SZP [Similar Products]
 UniProt Entry Name    PRG4_HUMAN
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 NCBI Summary for PRG4    The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
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 UniProt Comments for PRG4    PRG4: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Defects in PRG4 are the cause of camptodactyly- arthropathy-coxa vara-pericarditis syndrome (CACP); also known as Jacobs syndrome. CACP is an autosomal recessive disorder. Individuals with CACP have normal appearing joints at birth but with advancing age develop joint failure associated with noninflammatory synoviocyte hyperplasia and subintimal fibrosis of the synovial capsule. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q25-q31

Cellular Component: extracellular space

Molecular Function: scavenger receptor activity; polysaccharide binding

Biological Process: cell proliferation; receptor-mediated endocytosis; negative regulation of interleukin-6 biosynthetic process; immune response; regulation of cell proliferation

Disease: Camptodactyly-arthropathy-coxa Vara-pericarditis Syndrome
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 Research Articles on PRG4    1. 5 novel PRG4 mutations and the first case of CACP syndrome resulting from uniparental disomy of chromosome 1.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with PRG4 elisa kitOrgans/Tissues associated with PRG4 elisa kit
 Disease Name  Pubmed Publications
 Pericarditis Antibodies  >14 publications with PRG4 and Pericarditis
 Necrosis Antibodies  >6 publications with PRG4 and Necrosis
 Jacobs syndrome Antibodies  >4 publications with PRG4 and Jacobs syndrome
 Inflammation Antibodies  >4 publications with PRG4 and Inflammation
 Hyperplasia Antibodies  >4 publications with PRG4 and Hyperplasia
 Disease Models, Animal Antibodies  >3 publications with PRG4 and Disease Models, Animal
 Contracture Antibodies  >3 publications with PRG4 and Contracture
 Hypertrophy Antibodies  >2 publications with PRG4 and Hypertrophy
 Kidney Diseases Antibodies  >1 publications with PRG4 and Kidney Diseases
 Nervous System Diseases Antibodies  >1 publications with PRG4 and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >16 publications with PRG4 and Connective Tissue
 Vascular Antibodies  >5 publications with PRG4 and Vascular
 Embryonic Tissue Antibodies  >4 publications with PRG4 and Embryonic Tissue
 Eye Antibodies  >4 publications with PRG4 and Eye
 Liver Antibodies  >4 publications with PRG4 and Liver
 Adipose Tissue Antibodies  >2 publications with PRG4 and Adipose Tissue
 Heart Antibodies  >2 publications with PRG4 and Heart
 Lung Antibodies  >2 publications with PRG4 and Lung
 Brain Antibodies  >2 publications with PRG4 and Brain
 Thymus Antibodies  >1 publications with PRG4 and Thymus
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