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RBP4 elisa kit :: Guinea Pig RBP4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2504534
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
 
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 Product Name   

RBP4, ELISA Kit

★Popular Item★
 Also Known As   

Guinea pig RBP4 (Retinol Binding Protein 4, Plasma) ELISA Kit

 Product Gene Name   

RBP4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    180250
 3D Structure    ModBase 3D Structure for P02753
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 Species Reactivity    Guinea Pig
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 Specificity    This kit recognizes natural and recombinant Guinea pig RBP4. No significant cross-reactivity or interference between Guinea pig RBP4 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Sandwich
 Detection Range    7.813-500ng/mL
 Sensitivity    The minimum detectable dose of Guinea pig RBP4 is 4.688ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
 Intended Uses    This ELISA kit applies to the in vitro quantitative determination of Guinea pig RBP4 concentrations in serum, plasma and other biological fluids.
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RBP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RBP4 purchase    MBS2504534 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the RBP4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.813-500ng/mL in biological research samples containing RBP4, with an estimated sensitivity of The minimum detectable dose of Guinea pig RBP4 is 4.688ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero). The ELISA analytical biochemical technique of the MBS2504534 kit is based on RBP4 antibody-RBP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RBP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RBP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RBP4 elisa kit

   Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to RBP4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of RBP4. You can calculate the concentration of RBP4 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of RBP4 elisa kit    RBP4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2504534. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RBP4. It may not necessarily be applicable to this product.
 NCBI GI #    55743122
 NCBI GeneID    5950
 NCBI Accession #    NP_006735.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006744.3 [Other Products]
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 UniProt Primary Accession #    P02753 [Other Products]
 UniProt Secondary Accession #    O43478; O43479; Q5VY24; Q8WWA3; Q9P178; D3DR38 [Other Products]
 UniProt Related Accession #    P02753 [Other Products]
 Molecular Weight    23,010 Da
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 NCBI Official Full Name    retinol-binding protein 4
 NCBI Official Synonym Full Names    retinol binding protein 4, plasma
 NCBI Official Symbol    RBP4 [Similar Products]
 NCBI Official Synonym Symbols   
RDCCAS
[Similar Products]
 NCBI Protein Information    retinol-binding protein 4; PRBP; plasma retinol-binding protein; retinol-binding protein 4, interstitial
 UniProt Protein Name    Retinol-binding protein 4
 UniProt Synonym Protein Names   
Plasma retinol-binding protein; PRBP; RBP
 Protein Family    Retinol-binding protein
 UniProt Gene Name    RBP4 [Similar Products]
 UniProt Synonym Gene Names    PRBP; RBP [Similar Products]
 UniProt Entry Name    RET4_HUMAN
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 NCBI Summary for RBP4    This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]
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 UniProt Comments for RBP4    RBP4: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency). This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: extracellular space; protein complex; extracellular region; cytosol

Molecular Function: protein binding; protein heterodimerization activity; retinal binding; retinol binding

Biological Process: cardiac muscle development; phototransduction, visible light; response to retinoic acid; retinal metabolic process; urinary bladder development; maintenance of gastrointestinal epithelium; embryonic skeletal development; heart development; male gonad development; positive regulation of insulin secretion; vagina development; positive regulation of immunoglobulin secretion; uterus development; glucose homeostasis; gluconeogenesis; response to insulin stimulus; embryonic organ morphogenesis; response to ethanol; eye development; negative regulation of cardiac muscle cell proliferation; retinol metabolic process; embryonic retina morphogenesis in camera-type eye; detection of light stimulus involved in visual perception; spermatogenesis; retinoid metabolic process; female genitalia morphogenesis; lung development

Disease: Retinal Dystrophy, Iris Coloboma, And Comedogenic Acne Syndrome; Microphthalmia, Isolated, With Coloboma 10
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 Research Articles on RBP4    1. Two RBP4 SNPs showed associations with insulin resistance and insulin levels in women with prior gestational diabetes mellitus.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RBP4 elisa kitPathways associated with RBP4 elisa kit
 Reference Product  PubMed Publications
 TTR elisa kit  >34 publications with RBP4 and TTR
 STRA6 elisa kit  >18 publications with RBP4 and STRA6
 RETN elisa kit  >8 publications with RBP4 and RETN
 AHSG elisa kit  >5 publications with RBP4 and AHSG
 ALB elisa kit  >3 publications with RBP4 and ALB
 CYP2C9 elisa kit  >2 publications with RBP4 and CYP2C9
 AMBP elisa kit  >2 publications with RBP4 and AMBP
 PSMB1 elisa kit  >2 publications with RBP4 and PSMB1
 RPE65 elisa kit  >1 publications with RBP4 and RPE65
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 Retinoid Cycle Disease Events Pathway antibodies  Retinoid Cycle Disease Events Pathway Diagram
 Retinoid Metabolism And Transport Pathway antibodies  Retinoid Metabolism And Transport Pathway Diagram
 SREBP Signalling Pathway antibodies  SREBP Signalling Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 The Canonical Retinoid Cycle In Rods (twilight Vision) Pathway antibodies  The Canonical Retinoid Cycle In Rods (twilight Vision) Pathway Diagram
 Visual Phototransduction Pathway antibodies  Visual Phototransduction Pathway Diagram
 Vitamin A And Carotenoid Metabolism Pathway antibodies  Vitamin A And Carotenoid Metabolism Pathway Diagram
 Retinoate Biosynthesis I Pathway antibodies  Retinoate Biosynthesis I Pathway Diagram
Diseases associated with RBP4 elisa kitOrgans/Tissues associated with RBP4 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >86 publications with RBP4 and Inflammation
 Liver Diseases Antibodies  >67 publications with RBP4 and Liver Diseases
 Fatty Liver Antibodies  >61 publications with RBP4 and Fatty Liver
 Necrosis Antibodies  >53 publications with RBP4 and Necrosis
 Hyperglycemia Antibodies  >47 publications with RBP4 and Hyperglycemia
 Kidney Diseases Antibodies  >35 publications with RBP4 and Kidney Diseases
 Glucose Intolerance Antibodies  >33 publications with RBP4 and Glucose Intolerance
 Polycystic Ovary Syndrome Antibodies  >25 publications with RBP4 and Polycystic Ovary Syndrome
 Weight Loss Antibodies  >24 publications with RBP4 and Weight Loss
 Proteinuria Antibodies  >18 publications with RBP4 and Proteinuria
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >148 publications with RBP4 and Liver
 Connective Tissue Antibodies  >108 publications with RBP4 and Connective Tissue
 Muscle Antibodies  >62 publications with RBP4 and Muscle
 Kidney Antibodies  >36 publications with RBP4 and Kidney
 Heart Antibodies  >27 publications with RBP4 and Heart
 Bone Antibodies  >16 publications with RBP4 and Bone
 Pancreas Antibodies  >14 publications with RBP4 and Pancreas
 Brain Antibodies  >12 publications with RBP4 and Brain
 Embryonic Tissue Antibodies  >11 publications with RBP4 and Embryonic Tissue
 Eye Antibodies  >10 publications with RBP4 and Eye
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