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SNCa elisa kit :: Guinea Pig Synuclein, Alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721294
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Synuclein, Alpha (SNCa), ELISA Kit

★Popular Item★
 Also Known As   

Guinea Pig Synuclein, Alpha ELISA Kit

 Product Gene Name   

SNCa elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 4; NC_000004.11 (90645250..90759447, complement). Location: 4q21
 OMIM    127750
 3D Structure    ModBase 3D Structure for P37840
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 Species Reactivity    Guinea Pig
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 Specificity    This assay has high sensitivity and excellent specificity for detection of SNCalpha. No significant cross-reactivity or interference between SNCalpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SNCalpha and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Sandwich
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SNCa elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SNCa purchase    MBS721294 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Synuclein, Alpha (SNCa) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SNCa, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS721294 kit is based on SNCa antibody-SNCa antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SNCa antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SNCa. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for SNCa elisa kit

   Intended Uses: This SNCalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig SNCalpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: SNCalpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SNCalpha. Standards or samples are then added to the microtiter plate wells and SNCalpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SNC alpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SNCalpha are added to each well to "sandwich" the SNCalpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SNCalpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SNCalpha concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for SNCa elisa kit    Neurobiology
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 Typical Standard Curve/Testing Data of SNCa elisa kit    SNCa elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS721294. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SNCa. It may not necessarily be applicable to this product.
 NCBI GI #    49456267
 NCBI GeneID    6622
 NCBI Accession #    CAG46454.1 [Other Products]
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 UniProt Primary Accession #    P37840 [Other Products]
 UniProt Secondary Accession #    Q13701; Q4JHI3; Q6IAU6; A8K2A4 [Other Products]
 UniProt Related Accession #    P37840; Q4W5L2; Q6QBS3 [Other Products]
 Molecular Weight    14,460 Da
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 NCBI Official Full Name    SNCA
 NCBI Official Synonym Full Names    synuclein, alpha (non A4 component of amyloid precursor)
 NCBI Official Symbol    SNCA [Similar Products]
 NCBI Official Synonym Symbols   
PD1; NACP; PARK1; PARK4
[Similar Products]
 NCBI Protein Information    alpha-synuclein; synuclein alpha-140; non A-beta component of AD amyloid
 UniProt Protein Name    Alpha-synuclein
 UniProt Synonym Protein Names   
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor
 Protein Family    Alpha-synuclein
 UniProt Gene Name    SNCA [Similar Products]
 UniProt Synonym Gene Names    NACP; PARK1; NACP [Similar Products]
 UniProt Entry Name    SYUA_HUMAN
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 NCBI Summary for SNCa    Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq, Mar 2009]
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 UniProt Comments for SNCa    Function: May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.

Subunit structure: Soluble monomer which can form filamentous aggregates. Interacts with UCHL1

By similarity. Interacts with phospholipase D and histones. Ref.16 Ref.17

Subcellular location: Cytoplasm. Membrane. Nucleus. Cell junction › synapse. Note: Membrane-bound in dopaminergic neurons. Ref.17 Ref.21

Tissue specificity: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.

Domain: The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. Ref.22

Post-translational modification: Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress. Ref.13 Ref.14 Ref.15 Ref.20Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.Ubiquitinated. The predominant conjugate is the diubiquitinated form

By similarity.

Involvement in disease: Note=Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.Defects in SNCA are the cause of Parkinson disease type 1 (PARK1) [

MIM:168601]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. Additional features are characteristic postural abnormalities, dysautonomia, dystonic cramps, and dementia. The pathology of Parkinson disease involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. The disease is progressive and usually manifests after the age of 50 years, although early-onset cases (before 50 years) are known. The majority of the cases are sporadic suggesting a multifactorial etiology based on environmental and genetic factors. However, some patients present with a positive family history for the disease. Familial forms of the disease usually begin at earlier ages and are associated with atypical clinical features. Ref.25 Ref.26 Ref.27Defects in SNCA are the cause of Parkinson disease type 4 (PARK4) [

MIM:605543]. A complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.Defects in SNCA are the cause of dementia Lewy body (DLB) [

MIM:127750]. A neurodegenerative disorder clinically characterized by mental impairment leading to dementia, parkinsonism, often with fluctuating cognitive function, visual hallucinations, falls, syncopal episodes, and sensitivity to neuroleptic medication. Brainstem or cortical intraneuronal accumulations of aggregated proteins (Lewy bodies) are the only essential pathologic features. Patients may also have hippocampal and neocortical senile plaques, sometimes in sufficient number to fulfill the diagnostic criteria for Alzheimer disease.

Sequence similarities: Belongs to the synuclein family.
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 Research Articles on SNCa    1. Our findings strongly confirm SNCA and MAPT as major Parkinson disease susceptibility genes
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SNCa elisa kitPathways associated with SNCa elisa kit
 Reference Product  PubMed Publications
 SNCB elisa kit  >73 publications with SNCa and SNCB
 MAPT elisa kit  >53 publications with SNCa and MAPT
 SNCAIP elisa kit  >30 publications with SNCa and SNCAIP
 PARK2 elisa kit  >21 publications with SNCa and PARK2
 APP elisa kit  >16 publications with SNCa and APP
 SLC6A3 elisa kit  >12 publications with SNCa and SLC6A3
 MAPK1 elisa kit  >5 publications with SNCa and MAPK1
 TOR1A elisa kit  >4 publications with SNCa and TOR1A
 BAD elisa kit  >3 publications with SNCa and BAD
 SQSTM1 elisa kit  >3 publications with SNCa and SQSTM1
 Products by Pathway  Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Parkinson's Disease Pathway antibodies  Parkinson's Disease Pathway Diagram
Diseases associated with SNCa elisa kitOrgans/Tissues associated with SNCa elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >912 publications with SNCa and Nervous System Diseases
 Neurodegenerative Diseases Antibodies  >893 publications with SNCa and Neurodegenerative Diseases
 Parkinsonian Disorders Antibodies  >712 publications with SNCa and Parkinsonian Disorders
 Parkinson Disease Antibodies  >647 publications with SNCa and Parkinson Disease
 Lewy Body Disease Antibodies  >251 publications with SNCa and Lewy Body Disease
 Atrophy Antibodies  >152 publications with SNCa and Atrophy
 Death Antibodies  >152 publications with SNCa and Death
 Nerve Degeneration Antibodies  >151 publications with SNCa and Nerve Degeneration
 Alzheimer Disease Antibodies  >122 publications with SNCa and Alzheimer Disease
 Neoplasms Antibodies  >50 publications with SNCa and Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >967 publications with SNCa and Nerve
 Brain Antibodies  >624 publications with SNCa and Brain
 Blood Antibodies  >44 publications with SNCa and Blood
 Embryonic Tissue Antibodies  >26 publications with SNCa and Embryonic Tissue
 Kidney Antibodies  >18 publications with SNCa and Kidney
 Eye Antibodies  >7 publications with SNCa and Eye
 Heart Antibodies  >6 publications with SNCa and Heart
 Liver Antibodies  >5 publications with SNCa and Liver
 Bone Antibodies  >5 publications with SNCa and Bone
 Lung Antibodies  >3 publications with SNCa and Lung
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