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TGL elisa kit :: Guinea Pig Triglyceride Lipase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS737472
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Triglyceride Lipase (TGL), ELISA Kit

 Also Known As   

Guinea pig Triglyceride Lipase ELISA Kit

 Product Gene Name   

TGL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    614025
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 Species Reactivity    Guinea Pig
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    5.0-100nmoL/L
 Sensitivity    1.0nmoL/L
 Intended Uses    This TGL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig TGL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TGL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TGL purchase    MBS737472 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Triglyceride Lipase (TGL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5.0-100nmoL/L in biological research samples containing TGL, with an estimated sensitivity of 1.0nmoL/L. The ELISA analytical biochemical technique of the MBS737472 kit is based on TGL antibody-TGL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TGL elisa kit

   Principle of the Assay: TGL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TGL antibody and an TGL-HRP conjugate. The assay sample and buffer are incubated together with TGL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGL concentration since TGL from samples and TGL-HRP conjugate compete for the anti-TGL antibody binding site. Since the number of sites is limited, as more sites are occupied by TGL from the sample, fewer sites are left to bind TGL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGL concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TGL elisa kit    TGL elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS737472. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TGL. It may not necessarily be applicable to this product.
 NCBI GI #    339593
 NCBI GeneID    3990
 NCBI Accession #    AAB60702.1 [Other Products]
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 UniProt Secondary Accession #    O43571; P78529; Q99465; A2RUB4; A8K9B6 [Other Products]
 UniProt Related Accession #    P11150 [Other Products]
 Molecular Weight    55,914 Da
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 NCBI Official Full Name    triglyceride lipase
 NCBI Official Synonym Full Names    lipase, hepatic
 NCBI Official Symbol    LIPC [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    hepatic triacylglycerol lipase; hepatic lipase; lipase member C
 UniProt Protein Name    Hepatic triacylglycerol lipase
 UniProt Synonym Protein Names   
Lipase member C
 UniProt Gene Name    LIPC [Similar Products]
 UniProt Synonym Gene Names    HTGL; HL; Hepatic lipase [Similar Products]
 UniProt Entry Name    LIPC_HUMAN
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 NCBI Summary for TGL    LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TGL    LIPC: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency). A disorder characterized by elevated levels of beta-migrating very low density lipoproteins, and abnormally triglyceride-rich low and high density lipoproteins. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Secreted, signal peptide; Secreted; Lipid Metabolism - glycerolipid; Phospholipase; EC

Chromosomal Location of Human Ortholog: 15q21-q23

Cellular Component: extracellular space

Molecular Function: heparin binding; triacylglycerol lipase activity; low-density lipoprotein binding; apolipoprotein binding; phospholipase activity

Biological Process: cholesterol metabolic process; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol catabolic process; fatty acid biosynthetic process

Disease: High Density Lipoprotein Cholesterol Level Quantitative Trait Locus 12; Hepatic Lipase Deficiency; Diabetes Mellitus, Noninsulin-dependent
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 Research Articles on TGL    1. The findings suggest that LIPC and LPL genes could both modify the risk for AMD and the metabolism of lutein and zeaxanthin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TGL elisa kitPathways associated with TGL elisa kit
 Reference Product  PubMed Publications
 APOE elisa kit  >41 publications with TGL and APOE
 APOB elisa kit  >38 publications with TGL and APOB
 LDLR elisa kit  >15 publications with TGL and LDLR
 LIPF elisa kit  >1 publications with TGL and LIPF
 DGAT1 elisa kit  >1 publications with TGL and DGAT1
 Products by Pathway  Pathway Diagram
 Acylglycerol Degradation Pathway antibodies  Acylglycerol Degradation Pathway Diagram
 Acylglycerol Degradation Pathway antibodies  Acylglycerol Degradation Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Fatty Acid Beta Oxidation Pathway antibodies  Fatty Acid Beta Oxidation Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
Diseases associated with TGL elisa kitOrgans/Tissues associated with TGL elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >83 publications with TGL and Cardiovascular Diseases
 Atherosclerosis Antibodies  >45 publications with TGL and Atherosclerosis
 Dyslipidemias Antibodies  >34 publications with TGL and Dyslipidemias
 Brain Diseases Antibodies  >25 publications with TGL and Brain Diseases
 Hyperlipidemias Antibodies  >23 publications with TGL and Hyperlipidemias
 Insulin Resistance Antibodies  >20 publications with TGL and Insulin Resistance
 Diabetes Mellitus, Type 2 Antibodies  >19 publications with TGL and Diabetes Mellitus, Type 2
 Liver Diseases Antibodies  >14 publications with TGL and Liver Diseases
 Fatty Liver Antibodies  >11 publications with TGL and Fatty Liver
 Hypertriglyceridemia Antibodies  >10 publications with TGL and Hypertriglyceridemia
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >81 publications with TGL and Liver
 Brain Antibodies  >48 publications with TGL and Brain
 Heart Antibodies  >44 publications with TGL and Heart
 Lung Antibodies  >9 publications with TGL and Lung
 Kidney Antibodies  >6 publications with TGL and Kidney
 Mammary Gland Antibodies  >1 publications with TGL and Mammary Gland
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