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NOS3 elisa kit :: Guinea Pig nitric oxide synthase 3 (endothelial cell) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703677
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
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 Product Name   

nitric oxide synthase 3 (endothelial cell) (NOS3), ELISA Kit

 Also Known As   

Guinea Pig Endothelial nitric oxide synthase, eNOS ELISA Kit

 Product Synonym Names    Guinea Pig Endothelial nitric oxide synthase; eNOS ELISA Kit; ECNOS; eNOS; nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell)
 Product Gene Name   

NOS3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703677 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P97270
 Species Reactivity    Guinea Pig
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Guinea pig NOS3. No significant cross-reactivity or interference between Guinea pig NOS3 and analogues was observed.
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 Assay Type    Competitive
 Detection Range    500 pg/ml-8000 pg/ml
 Sensitivity    250 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NOS3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NOS3 purchase    MBS703677 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nitric oxide synthase 3 (endothelial cell) (NOS3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 500 pg/ml-8000 pg/ml in biological research samples containing NOS3, with an estimated sensitivity of 250 pg/ml. The ELISA analytical biochemical technique of the MBS703677 kit is based on NOS3 antibody-NOS3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NOS3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NOS3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NOS3 elisa kit

   Introduction: Nitric oxide (NO) is produced by a group of enzymes called nitric oxide synthases (NOS). These enzymes catalyze the production of NO and L-citrulline from L-arginine, O2, and NADPH derived electrons. Mammalian systems contain three well-characterized isoforms of the enzyme: neuronal NOS (nNOS, also called NOS1), inducible NOS (iNOS or NOS2), and endothelial NOS (eNOS or NOS3). The cell types that express eNOS include vascular endothelial cells, cardiomyocytes and others. In blood vessels, NO produced by the eNOS in endothelial cells functions as a vasodilator thereby regulating blood flow and pressure. Mutant eNOS knockout mice have blood pressure that is 30% higher than wild-type littermates. Within cardiomyocytes, eNOS affects Ca2+ currents and contractility. Expression of eNOS is usually reported to be constitutive, though modest degrees of regulation occur in response to factors such as shear stress, exercise, chronic hypoxia, and heart failure. The unique N-terminal sequence of eNOS is about 70 residues long and functions to localize the enzyme to membranes. Upon myristoylation at one site and palmitoylation at two other sites within this segment, the enzyme is exclusively membrane-bound. 3 Palmitoylation is a reversible process that is influenced by some agonists and is essential for membrane localization. Within the membrane, eNOS is targeted to the caveolae, small invaginations characterized by the presence of proteins called caveolins. These regions serve as sites for the sequestration of signaling molecules such as receptors, G proteins and protein kinases. The oxygenase domain of eNOS contains a motif that binds to caveolin-1, and calmodulin is believed to competitively displace caveolin resulting in eNOS activation. Bound calmodulin is required for activity of eNOS, and this binding occurs in response to transient increases in intracellular Ca2+. Thus, eNOS occurs at sites of signal transduction and produces short pulses of NO in response to agonists that elicit Ca2+ transients. Physiological concentrations of eNOS-derived NO are in the picomolar range . Within the cardiovascular system, eNOS generally has protective effects. Studies with nNOS and eNOS knockout mice clearly indicate that eNOS plays a protective role in cerebral is chemia by preserving cerebral blood flow. During inflammation and atherosclerosis, low concentrations of NO prevent apoptotic death of endothelial cells and preserve the integrity of the endothelial cell monolayer. NO also acts as an inhibitor of 4 platelet aggregation, adhesion molecule expression, and vascular smooth muscle cell proliferation.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to eNOS has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated eNOS and incubated. A competitive inhibition reaction is launched between eNOS (Standards or samples) and Biotin-conjugated eNOS with the pre-coated antibody specific for eNOS. The more amount of eNOS in samples, the less antibody bound by Biotin-conjugated ENOS. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of eNOS in the sample. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NOS3 elisa kit    NOS3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703677. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NOS3. It may not necessarily be applicable to this product.
 NCBI GI #    8473528
 NCBI Accession #    P97270.1 [Other Products]
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 UniProt Primary Accession #    P97270 [Other Products]
 Molecular Weight    11,271 Da
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 NCBI Official Full Name    Nitric oxide synthase, endothelial
 UniProt Protein Name    Nitric oxide synthase, endothelial
 UniProt Synonym Protein Names   
Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
 Protein Family    Nitric oxide synthase
 UniProt Gene Name    NOS3 [Similar Products]
 UniProt Synonym Gene Names    ENOS; cNOS; eNOS; NOSIII [Similar Products]
 UniProt Entry Name    NOS3_CAVPO
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 UniProt Comments for NOS3    Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group

Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NOS3 elisa kitPathways associated with NOS3 elisa kit
 Reference Product  PubMed Publications
 AKT1 elisa kit  >123 publications with NOS3 and AKT1
 ARG2 elisa kit  >16 publications with NOS3 and ARG2
 ARG1 elisa kit  >11 publications with NOS3 and ARG1
 AKT2 elisa kit  >5 publications with NOS3 and AKT2
 NOSTRIN elisa kit  >4 publications with NOS3 and NOSTRIN
 AKT3 elisa kit  >2 publications with NOS3 and AKT3
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