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HABP elisa kit :: GuineaPig Hyaluronate binding protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS732995
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hyaluronate binding protein (HABP), ELISA Kit

 Also Known As   

Guinea Pig Hyaluronate binding protein ELISA Kit

 Product Synonym Names    Guinea Pig Hyaluronate binding protein ELISA Kit; Hyaluronate binding protein; Hyaluronate binding protein (Guinea Pig)
 Product Gene Name   

HABP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Chromosome Location    Chromosome: 2; NC_000002.11 (152214106..152236562). Location: 2q23.3
 OMIM    600410
 3D Structure    ModBase 3D Structure for P98066
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 Species Reactivity    GuineaPig
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 Specificity    This assay has high sensitivity and excellent specificity for detection of HABP. No significant cross-reactivity or interference between HABP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HABP and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    0.1 ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of HABP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HABP purchase    MBS732995 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hyaluronate binding protein (HABP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HABP, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS732995 kit is based on HABP antibody-HABP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HABP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HABP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for HABP elisa kit

   Intended Uses: This HABP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig HABP.

Principle of the Assay: HABP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HABP antibody and an HABP-HRP conjugate. The assay sample and buffer are incubated together with HABP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HABP concentration since HABP from samples and HABP-HRP conjugate compete for the anti-HABP antibody binding site. Since the number of sites is limited, as more sites are occupied by HABP from the sample, fewer sites are left to bind HABP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HABP concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for HABP elisa kit    Guinea Pig ELISA Kit
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 Typical Testing Data/Standard Curve (for reference only) of HABP elisa kit    HABP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS732995
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NCBI/Uniprot data below describe general gene information for HABP. It may not necessarily be applicable to this product.
 NCBI GI #    26051243
 NCBI GeneID    7130
 NCBI Accession #    NP_009046.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_007115.3 [Other Products]
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 UniProt Primary Accession #    P98066 [Other Products]
 UniProt Secondary Accession #    Q53TI7; Q8WWI9 [Other Products]
 UniProt Related Accession #    P98066 [Other Products]
 Molecular Weight    31,203 Da
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 NCBI Official Full Name    tumor necrosis factor-inducible gene 6 protein
 NCBI Official Synonym Full Names    tumor necrosis factor, alpha-induced protein 6
 NCBI Official Symbol    TNFAIP6 [Similar Products]
 NCBI Official Synonym Symbols   
TSG6; TSG-6
[Similar Products]
 NCBI Protein Information    tumor necrosis factor-inducible gene 6 protein; TNF alpha-induced protein 6; hyaluronate-binding protein; TNF-stimulated gene 6 protein; tumor necrosis factor alpha-inducible protein 6; tumor necrosis factor-stimulated gene-6 protein
 UniProt Protein Name    Tumor necrosis factor-inducible gene 6 protein
 UniProt Synonym Protein Names   
Hyaluronate-binding protein; TNF-stimulated gene 6 protein; TSG-6; Tumor necrosis factor alpha-induced protein 6
 UniProt Gene Name    TNFAIP6 [Similar Products]
 UniProt Synonym Gene Names    TSG6; TSG-6 [Similar Products]
 UniProt Entry Name    TSG6_HUMAN
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 NCBI Summary for HABP    The protein encoded by this gene is a secretory protein that contains a hyaluronan-binding domain, and thus is a member of the hyaluronan-binding protein family. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable complex with inter-alpha-inhibitor (I alpha I), and thus enhance the serine protease inhibitory activity of I alpha I, which is important in the protease network associated with inflammation. This gene can be induced by proinflammatory cytokines such as tumor necrosis factor alpha and interleukin-1. Enhanced levels of this protein are found in the synovial fluid of patients with osteoarthritis and rheumatoid arthritis.[provided by RefSeq, Dec 2010]
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 UniProt Comments for HABP    TNFAIP6: Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.

Chromosomal Location of Human Ortholog: 2q23.3

Cellular Component: extracellular space

Molecular Function: hyaluronic acid binding

Biological Process: ovulation; cell-cell signaling; cell adhesion; inflammatory response; signal transduction; positive regulation of cell migration
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 Research Articles on HABP    1. The protein product of tumor necrosis factor-alpha stimulated gene-6 strongly correlated with ossification markers and hyaluronidase in calcified human aortic valves.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HABP elisa kitDiseases associated with HABP elisa kit
 Reference Product  PubMed Publications
 PTX3 elisa kit  >16 publications with HABP and PTX3
 THBS1 elisa kit  >3 publications with HABP and THBS1
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >101 publications with HABP and Necrosis
 Inflammation Antibodies  >66 publications with HABP and Inflammation
 Neoplasms Antibodies  >11 publications with HABP and Neoplasms
 Lung Diseases Antibodies  >8 publications with HABP and Lung Diseases
 Cardiovascular Diseases Antibodies  >6 publications with HABP and Cardiovascular Diseases
 Skin Diseases Antibodies  >5 publications with HABP and Skin Diseases
 Nervous System Diseases Antibodies  >4 publications with HABP and Nervous System Diseases
 Heart Diseases Antibodies  >3 publications with HABP and Heart Diseases
 Kidney Diseases Antibodies  >3 publications with HABP and Kidney Diseases
 Adenocarcinoma Antibodies  >3 publications with HABP and Adenocarcinoma
Organs/Tissues associated with HABP elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >46 publications with HABP and Blood
 Bone Antibodies  >20 publications with HABP and Bone
 Muscle Antibodies  >11 publications with HABP and Muscle
 Vascular Antibodies  >10 publications with HABP and Vascular
 Lung Antibodies  >10 publications with HABP and Lung
 Eye Antibodies  >8 publications with HABP and Eye
 Kidney Antibodies  >8 publications with HABP and Kidney
 Connective Tissue Antibodies  >7 publications with HABP and Connective Tissue
 Pancreas Antibodies  >5 publications with HABP and Pancreas
 Heart Antibodies  >5 publications with HABP and Heart
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