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HAMA elisa kit

Scan QR to view Datasheet Catalog #    MBS590048
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Wells  /  $605 +1 FREE 8GB USB
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HAMA elisa kit

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 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
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 Samples    Serum
 Assay Type    Sandwich
 Intra-assay Precision    Three different testing samples of known concentration were assayed twenty times on one plate to assess intra-assay precision.
 Inter-assay Precision    Three different samples of known concentration were assayed in 20 different separate assays to assess inter-assay precision.
 Intended Uses    This HAMA ELISA is to be used for the quantitative determination of human anti-mouse antibody (HAMA) in serum. It is useful for the prognosis, monitoring and follow-up patients treated with mouse IgG. This kit is intended for LABORTAROY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.
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 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HAMA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HAMA purchase    MBS590048 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the HAMA, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HAMA. The ELISA analytical biochemical technique of the MBS590048 kit is based on HAMA antibody-HAMA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HAMA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HAMA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HAMA elisa kit

   Principle of the assay: This assay applies an enzyme-linked immunosorbent assay (ELISA) technique. A purified mouse IgG has been pre-coated onto the wells of microplate. Standard (rabbit anti-mouse IgG) and specimens are added into the appropriate wells simultaneously with a HRP-labelled mouse IgG, and incubated. The Human Anti-Mouse Antibody (HAMA), if present, will bind with the mouse IgG which coated on the wells and HRPlabelled mouse IgG as well, forming a "Sandwich". Following a wash to remove any unbound substance, a substrate solution is added. The developed colour is directly proportional to the anti-mouse antibody concentration in the specimen and Standard samples. This ELISA Kit includes a Calibration Diluent for preparing a 2-fold dilution of Standard series. It allows the operator to produce a standard curve with Optical Density (O.D) versus Human Anti-Mouse Antibody concentration (ng/mL). The concentration of Human Anti-Mouse Antibody in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Background: Human Anti-Mouse Antibody (HAMA) are antibodies found in human serum which have the ability to bind to mouse immunoglobulin G (IgG). The presence of HAMA is the result of an immune reaction following an exposure to mice or other similar agent, which was able to induce the immune system to generate an antibody able to bind to mouse IgG. HAMA is commonly found in patients following treated with mouse monoclonal antibodies associated with some therapeutic or diagnostic procedures. Some human auto-antibodies, the most common of which is rheumatoid factor (Rf), by virtue of their cross reactivity are able to bind mouse IgG. The HAMA assay measures HAMA across a wide dynamic range and it is not necessary to predilute human serum samples before the HAMA ELISA testing.
 Product Categories/Family for HAMA elisa kit    ELISA Kits for Human Inflammatory and Autoimmune Conditions
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 Typical Testing Data/Standard Curve (for reference only) of HAMA elisa kit    HAMA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS590048. Click to request current manual
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 Protein Family    Hamartin
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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