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ALOX-5 elisa kit :: Hamster Arachidonate 5-Lipoxygenase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS013475 ALOX-5 elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Arachidonate 5-Lipoxygenase (ALOX-5), ELISA Kit

 Also Known As   

Hamster Arachidonate 5-Lipoxygenase ELISA Kit

 Product Gene Name   

ALOX-5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    chromosome: Un
 3D Structure    ModBase 3D Structure for P51399
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 Species Reactivity    Hamster
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 Assay Type    Sandwich
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ALOX-5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ALOX-5 purchase    MBS013475 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Arachidonate 5-Lipoxygenase (ALOX-5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ALOX-5. The ELISA analytical biochemical technique of the MBS013475 kit is based on ALOX-5 antibody-ALOX-5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALOX-5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALOX-5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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NCBI/Uniprot data below describe general gene information for ALOX-5. It may not necessarily be applicable to this product.
 NCBI GI #    528078462
 NCBI GeneID    101839970
 NCBI Accession #    NP_001268516.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001281587.1 [Other Products]
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 UniProt Primary Accession #    P51399 [Other Products]
 UniProt Related Accession #    P51399 [Other Products]
 Molecular Weight    77,873 Da
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 NCBI Official Full Name    arachidonate 5-lipoxygenase
 NCBI Official Symbol    Alox5 [Similar Products]
 NCBI Official Synonym Symbols   
5-LO
[Similar Products]
 NCBI Protein Information    arachidonate 5-lipoxygenase
 UniProt Protein Name    Arachidonate 5-lipoxygenase
 Protein Family    Arachidonate 5-lipoxygenase
 UniProt Gene Name    ALOX5 [Similar Products]
 UniProt Synonym Gene Names    5-LO [Similar Products]
 UniProt Entry Name    LOX5_MESAU
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 UniProt Comments for ALOX-5    Function: Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Catalytic activity: Arachidonate + O2 = leukotriene A4 + H2O.

Cofactor: Binds 1 iron ion per subunit

By similarity.Binds 2 calcium ions per subunit

By similarity.

Pathway: Lipid metabolism; leukotriene A4 biosynthesis.

Subunit structure: Interacts with ALOX5AP and LTC4S. Interacts with COTL1, the interaction is required for stability and efficient catalytic activity

By similarity.

Subcellular location: Cytoplasm

By similarity. Nucleus matrix

By similarity. Nucleus membrane; Peripheral membrane protein

By similarity. Note: Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-271. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association

By similarity.

Post-translational modification: Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-271 prevents export from the nucleus

By similarity.

Sequence similarities: Belongs to the lipoxygenase family.Contains 1 lipoxygenase domain.Contains 1 PLAT domain.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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