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eIF2alpha elisa kit

Scan QR to view Datasheet Catalog #    MBS9501438 eIF2alpha elisa kit
Unit / Price
2x96 Wells  /  $535 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

eIF2alpha, ELISA Kit

★Popular Item★
 Also Known As   

Phospho-eIF2alpha (Ser51) Colorimetric Cell-Based ELISA Kit

 Product Synonym Names    eIF-2-alpha; EIF-2A; EIF-2alpha; EIF2S1; Eukaryotic translation initiation factor 2 alpha subunit; Eukaryotic translation initiation factor 2 subunit 1; IF2A
 Product Gene Name   

eIF2alpha elisa kit

[Similar Products]
 Product Synonym Gene Name    EIF2S1 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    J02645 mRNA
 3D Structure    ModBase 3D Structure for P05198
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 Species Reactivity    Human, Mouse, Rat
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 Form/Format    Two 96-Well Plates
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 Samples    Researchers plate their cell line of choice
 Assay Type    Phospho Cell-Based ELISA
 Sensitivity    >=5000 cells/well
 Reactivity Note    Human: S52, Mouse: S52, Rat: S52
 Detection Method    Colorimetric 450 nm
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 Preparation and Storage    Store at 4 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of eIF2alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for eIF2alpha purchase    MBS9501438 is a ready-to-use microwell, strip-or-full plate Phospho Cell-Based ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the eIF2alpha, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing eIF2alpha, with an estimated sensitivity of >=5000 cells/well. The ELISA analytical biochemical technique of the MBS9501438 kit is based on eIF2alpha antibody-eIF2alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect eIF2alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, eIF2alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Researchers plate their cell line of choice. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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 Product Categories/Family for eIF2alpha elisa kit    Cell Based ELISA Kit
Sample Manual Insert of MBS9501438. Click to request current manual
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NCBI/Uniprot data below describe general gene information for eIF2alpha. It may not necessarily be applicable to this product.
 NCBI GI #    4758256
 NCBI GeneID    1965
 NCBI Accession #    NP_004085.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004094.4 [Other Products]
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 UniProt Primary Accession #    P05198 [Other Products]
 UniProt Related Accession #    P05198 [Other Products]
 Molecular Weight    36112
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 NCBI Official Full Name    eukaryotic translation initiation factor 2 subunit 1
 NCBI Official Synonym Full Names    eukaryotic translation initiation factor 2 subunit alpha
 NCBI Official Symbol    EIF2S1 [Similar Products]
 NCBI Official Synonym Symbols   
EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
[Similar Products]
 NCBI Protein Information    eukaryotic translation initiation factor 2 subunit 1
 UniProt Protein Name    Eukaryotic translation initiation factor 2 subunit 1
 UniProt Synonym Protein Names   
Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
 UniProt Gene Name    EIF2S1 [Similar Products]
 UniProt Synonym Gene Names    EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha [Similar Products]
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 NCBI Summary for eIF2alpha    The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
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 UniProt Comments for eIF2alpha    eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections.

Protein type: RNA-binding; Translation; Translation initiation

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: cytosol; eukaryotic translation initiation factor 2 complex; eukaryotic translation initiation factor 2B complex; extracellular exosome; membrane; nucleus; polysome; ribosome; stress granule; translation initiation ternary complex

Molecular Function: protein binding; ribosome binding; RNA binding; translation initiation factor activity

Biological Process: aging; cellular response to amino acid starvation; cellular response to heat; cellular response to UV; negative regulation of guanyl-nucleotide exchange factor activity; negative regulation of translational initiation in response to stress; PERK-mediated unfolded protein response; positive regulation of neuron death; positive regulation of type B pancreatic cell apoptotic process; protein autophosphorylation; response to manganese-induced endoplasmic reticulum stress; translational initiation; transmembrane transport
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 Research Articles on eIF2alpha    1. Data show that eIF2Balpha and eIF2Bbeta bind to adjacent surfaces on eIF2alpha-N-terminal domains (NTDs).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with eIF2alpha elisa kitPathways associated with eIF2alpha elisa kit
 Reference Product  PubMed Publications
 EIF2AK3 elisa kit  >1 publications with eIF2alpha and EIF2AK3
 EIF2AK2 elisa kit  >1 publications with eIF2alpha and EIF2AK2
 Products by Pathway  Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway antibodies  Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
Diseases associated with eIF2alpha elisa kitOrgans/Tissues associated with eIF2alpha elisa kit
 Disease Name  Pubmed Publications
 Death Antibodies  >2 publications with eIF2alpha and Death
 Hyperglycemia Antibodies  >1 publications with eIF2alpha and Hyperglycemia
 Glucose Intolerance Antibodies  >1 publications with eIF2alpha and Glucose Intolerance
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >1 publications with eIF2alpha and Pancreas
 Blood Antibodies  >1 publications with eIF2alpha and Blood
 Brain Antibodies  >1 publications with eIF2alpha and Brain
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