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PHPT1 elisa kit :: Human 14 kDa phosphohistidine phosphatase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7232920 PHPT1 elisa kit
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

14 kDa phosphohistidine phosphatase (PHPT1), ELISA Kit

 Also Known As   

Human 14 kDa phosphohistidine phosphatase ELISA Kit

 Product Gene Name   

PHPT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    610167
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 Species Reactivity    Human
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    5.0-100ng/mL
 Sensitivity    1.0ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PHPT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PHPT1 purchase    MBS7232920 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 14 kDa phosphohistidine phosphatase (PHPT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5.0-100ng/mL in biological research samples containing PHPT1, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS7232920 kit is based on PHPT1 antibody-PHPT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PHPT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PHPT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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 Product Categories/Family for PHPT1 elisa kit    Cell Biology
NCBI/Uniprot data below describe general gene information for PHPT1. It may not necessarily be applicable to this product.
 NCBI GI #    565324201
 NCBI GeneID    29085
 NCBI Accession #    NP_001274271.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001287342.1 [Other Products]
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 UniProt Secondary Accession #    Q9H0Y3; B1AMX0; B1AMX1 [Other Products]
 UniProt Related Accession #    Q9NRX4 [Other Products]
 Molecular Weight    13,672 Da
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 NCBI Official Full Name    14 kDa phosphohistidine phosphatase isoform 4
 NCBI Official Synonym Full Names    phosphohistidine phosphatase 1
 NCBI Official Symbol    PHPT1 [Similar Products]
 NCBI Official Synonym Symbols   
PHP14; CGI-202; HSPC141; HEL-S-132P
[Similar Products]
 NCBI Protein Information    14 kDa phosphohistidine phosphatase; protein janus-A homolog; sex-regulated protein janus-a; epididymis secretory sperm binding protein Li 132P
 UniProt Protein Name    14 kDa phosphohistidine phosphatase
 UniProt Synonym Protein Names   
Phosphohistidine phosphatase 1; Protein janus-A homolog
 Protein Family    14 kDa phosphohistidine phosphatase
 UniProt Gene Name    PHPT1 [Similar Products]
 UniProt Synonym Gene Names    PHP14; CGI-202; HSPC141 [Similar Products]
 UniProt Entry Name    PHP14_HUMAN
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 NCBI Summary for PHPT1    This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
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 UniProt Comments for PHPT1    PHPT1: a phosphohistidine phosphatase. Present preferentially in heart and skeletal muscle. Insensitive to inhibition by okadaic acid and EDTA.

Protein type: Cofactor and Vitamin Metabolism - riboflavin; Carbohydrate Metabolism - fructose and mannose; EC 3.1.3.-; Phosphatase; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - thiamine

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytosol

Molecular Function: calcium channel inhibitor activity; phosphohistidine phosphatase activity; phosphoprotein phosphatase activity

Biological Process: negative regulation of lyase activity; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation
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 Research Articles on PHPT1    1. Overexpression of PHP by the use of a plasmid vector, pIRES2-AcGFP1-PHP, induced apoptosis in HUVEC
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PHPT1 elisa kitPathways associated with PHPT1 elisa kit
 Reference Product  PubMed Publications
 KCNN4 elisa kit  >2 publications with PHPT1 and KCNN4
 Products by Pathway  Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
Diseases associated with PHPT1 elisa kitOrgans/Tissues associated with PHPT1 elisa kit
 Disease Name  Pubmed Publications
 Lung Neoplasms Antibodies  >2 publications with PHPT1 and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with PHPT1 and Carcinoma, Hepatocellular
 Neoplasm Metastasis Antibodies  >1 publications with PHPT1 and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3 publications with PHPT1 and Blood
 Pancreas Antibodies  >2 publications with PHPT1 and Pancreas
 Liver Antibodies  >2 publications with PHPT1 and Liver
 Lung Antibodies  >2 publications with PHPT1 and Lung
 Heart Antibodies  >2 publications with PHPT1 and Heart
 Muscle Antibodies  >2 publications with PHPT1 and Muscle
 Kidney Antibodies  >1 publications with PHPT1 and Kidney
 Intestine Antibodies  >1 publications with PHPT1 and Intestine
 Lymph Node Antibodies  >1 publications with PHPT1 and Lymph Node
 Testis Antibodies  >1 publications with PHPT1 and Testis
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