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CYP27B1 elisa kit :: Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial ELISA Kit

Scan QR to view Datasheet Catalog #    MBS765246
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (CYP27B1), ELISA Kit

★Popular Item★
 Also Known As   

Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial ELISA Kit

 Product Synonym Names    CYP27B1/VD3 1A hydroxylase/Cytochrome P450VD1-alpha/Cytochrome P450C1 alpha/Cytochrome P450 subfamily XXVIIB polypeptide 1/Calcidiol 1-monooxygenase/Cytochrome p450 27B1/25-OHD-1 alpha-hydroxylase/25-hydroxyvitamin D (3) 1-alpha-hydroxylase
 Product Gene Name   

CYP27B1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS765246 COA    COA PDF
 OMIM    264700
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CYP27B1. No significant cross-reactivity or interference between CYP27B1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Detection Range    0.78-50ng/ml
 Sensitivity    <0.469ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CYP27B1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CYP27B1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CYP27B1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CYP27B1 purchase    MBS765246 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (CYP27B1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78-50ng/ml in biological research samples containing CYP27B1, with an estimated sensitivity of <0.469ng/ml. The ELISA analytical biochemical technique of the MBS765246 kit is based on CYP27B1 antibody-CYP27B1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP27B1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP27B1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CYP27B1 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CYP27B1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CYP27B1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CYP27B1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CYP27B1 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of CYP27B1 elisa kit    CYP27B1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS765246. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CYP27B1. It may not necessarily be applicable to this product.
 NCBI GI #    4503213
 NCBI GeneID    1594
 NCBI Accession #    NP_000776.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000785.3 [Other Products]
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 UniProt Secondary Accession #    Q548T3; B2RC61 [Other Products]
 UniProt Related Accession #    O15528 [Other Products]
 Molecular Weight    56,504 Da
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 NCBI Official Full Name    25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
 NCBI Official Synonym Full Names    cytochrome P450 family 27 subfamily B member 1
 NCBI Official Symbol    CYP27B1 [Similar Products]
 NCBI Official Synonym Symbols   
VDR; CP2B; CYP1; PDDR; VDD1; VDDR; VDDRI; CYP27B; P450c1; CYP1alpha
[Similar Products]
 NCBI Protein Information    25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
 UniProt Protein Name    25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
 UniProt Synonym Protein Names   
25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; VD3 1A hydroxylase; Calcidiol 1-monooxygenase; Cytochrome P450 subfamily XXVIIB polypeptide 1; Cytochrome P450C1 alpha; Cytochrome P450VD1-alpha; Cytochrome p450 27B1
 Protein Family    25-hydroxyvitamin D-1 alpha hydroxylase
 UniProt Gene Name    CYP27B1 [Similar Products]
 UniProt Synonym Gene Names    CYP1ALPHA; CYP27B; VD3 1A hydroxylase [Similar Products]
 UniProt Entry Name    CP27B_HUMAN
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 NCBI Summary for CYP27B1    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CYP27B1    CYP27B1: Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation. Defects in CYP27B1 are the cause of rickets vitamin D- dependent type 1A (VDDR1A); also known as pseudovitamin D deficiency rickets (PDDR). A disorder caused by a selective deficiency of the active form of vitamin D (1,25- dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. Belongs to the cytochrome P450 family.

Protein type: Cell cycle regulation; EC 1.14.13.13; Oxidoreductase; Mitochondrial; Lipid Metabolism - steroid biosynthesis

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: cytoplasm; mitochondrial outer membrane; mitochondrion

Molecular Function: calcidiol 1-monooxygenase activity; heme binding; iron ion binding

Biological Process: bone mineralization; calcium ion homeostasis; calcium ion transport; decidualization; fat-soluble vitamin metabolic process; negative regulation of cell growth; negative regulation of cell proliferation; positive regulation of keratinocyte differentiation; regulation of bone mineralization; response to estrogen stimulus; response to lipopolysaccharide; response to vitamin D; vitamin D catabolic process; vitamin D metabolic process; vitamin metabolic process; xenobiotic metabolic process

Disease: Vitamin D Hydroxylation-deficient Rickets, Type 1a
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 Research Articles on CYP27B1    1. Increased CYP27B1 expression and local duodenal 1,25(OH)2D3 production during puberty may be a metabolic adaptation that promotes dietary calcium absorption.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CYP27B1 elisa kitPathways associated with CYP27B1 elisa kit
 Reference Product  PubMed Publications
 CYP24A1 elisa kit  >425 publications with CYP27B1 and CYP24A1
 VDR elisa kit  >321 publications with CYP27B1 and VDR
 PTH elisa kit  >145 publications with CYP27B1 and PTH
 FGF23 elisa kit  >43 publications with CYP27B1 and FGF23
 TLR2 elisa kit  >12 publications with CYP27B1 and TLR2
 AHR elisa kit  >1 publications with CYP27B1 and AHR
 Products by Pathway  Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Cholecalciferol Biosynthesis Pathway antibodies  Cholecalciferol Biosynthesis Pathway Diagram
 Cholecalciferol Biosynthesis Pathway antibodies  Cholecalciferol Biosynthesis Pathway Diagram
 Cytochrome P450 - Arranged By Substrate Type Pathway antibodies  Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Fat-soluble Vitamins Pathway antibodies  Metabolism Of Fat-soluble Vitamins Pathway Diagram
 Metabolism Of Vitamins And Cofactors Pathway antibodies  Metabolism Of Vitamins And Cofactors Pathway Diagram
 Nuclear Receptors In Lipid Metabolism And Toxicity Pathway antibodies  Nuclear Receptors In Lipid Metabolism And Toxicity Pathway Diagram
 Phase 1 - Functionalization Of Compounds Pathway antibodies  Phase 1 - Functionalization Of Compounds Pathway Diagram
Diseases associated with CYP27B1 elisa kitOrgans/Tissues associated with CYP27B1 elisa kit
 Disease Name  Pubmed Publications
 Rickets Antibodies  >152 publications with CYP27B1 and Rickets
 Kidney Diseases Antibodies  >134 publications with CYP27B1 and Kidney Diseases
 Disease Models, Animal Antibodies  >57 publications with CYP27B1 and Disease Models, Animal
 Hypercalcemia Antibodies  >53 publications with CYP27B1 and Hypercalcemia
 Inflammation Antibodies  >50 publications with CYP27B1 and Inflammation
 Renal Insufficiency Antibodies  >47 publications with CYP27B1 and Renal Insufficiency
 Breast Neoplasms Antibodies  >41 publications with CYP27B1 and Breast Neoplasms
 Prostatic Neoplasms Antibodies  >41 publications with CYP27B1 and Prostatic Neoplasms
 Liver Diseases Antibodies  >34 publications with CYP27B1 and Liver Diseases
 Hypertension Antibodies  >25 publications with CYP27B1 and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >543 publications with CYP27B1 and Kidney
 Blood Antibodies  >383 publications with CYP27B1 and Blood
 Bone Antibodies  >277 publications with CYP27B1 and Bone
 Intestine Antibodies  >113 publications with CYP27B1 and Intestine
 Muscle Antibodies  >31 publications with CYP27B1 and Muscle
 Brain Antibodies  >31 publications with CYP27B1 and Brain
 Lung Antibodies  >30 publications with CYP27B1 and Lung
 Uterus Antibodies  >14 publications with CYP27B1 and Uterus
 Mammary Gland Antibodies  >12 publications with CYP27B1 and Mammary Gland
 Pancreas Antibodies  >8 publications with CYP27B1 and Pancreas
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