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MRPS2 elisa kit :: Human 28S ribosomal protein S2, mitochondrial (MRPS2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7250174
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
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 Product Name   

28S ribosomal protein S2, mitochondrial (MRPS2), ELISA Kit

★Popular Item★
 Also Known As   

Human 28S ribosomal protein S2, mitochondrial (MRPS2) ELISA Kit

 Product Gene Name   

MRPS2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    611971
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of MRPS2. No significant cross-reactivity or interference between MRPS2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MRPS2 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MRPS2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MRPS2 purchase    MBS7250174 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 28S ribosomal protein S2, mitochondrial (MRPS2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing MRPS2, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7250174 kit is based on MRPS2 antibody-MRPS2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MRPS2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MRPS2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MRPS2 elisa kit

   Intended Uses: This MRPS2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MRPS2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||MRPS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MRPS2 antibody and an MRPS2-HRP conjugate. The assay sample and buffer are incubated together with MRPS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MRPS2 concentration since MRPS2 from samples and MRPS2-HRP conjugate compete for the anti-MRPS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MRPS2 from the sample, fewer sites are left to bind MRPS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MRPS2 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve of MRPS2 elisa kit    MRPS2 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS7250174. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MRPS2. It may not necessarily be applicable to this product.
 NCBI GI #    7705805
 NCBI GeneID    51116
 NCBI Accession #    NP_057118.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_016034.4 [Other Products]
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 UniProt Secondary Accession #    Q5T899; Q9BSQ4 [Other Products]
 UniProt Related Accession #    Q9Y399 [Other Products]
 Molecular Weight    33,249 Da
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 NCBI Official Full Name    28S ribosomal protein S2, mitochondrial
 NCBI Official Synonym Full Names    mitochondrial ribosomal protein S2
 NCBI Official Symbol    MRPS2 [Similar Products]
 NCBI Official Synonym Symbols   
S2mt; CGI-91; MRP-S2
[Similar Products]
 NCBI Protein Information    28S ribosomal protein S2, mitochondrial
 UniProt Protein Name    28S ribosomal protein S2, mitochondrial
 Protein Family    28S ribosomal protein
 UniProt Gene Name    MRPS2 [Similar Products]
 UniProt Synonym Gene Names    CGI-91; MRP-S2; S2mt [Similar Products]
 UniProt Entry Name    RT02_HUMAN
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 NCBI Summary for MRPS2    Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S2 family. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
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 UniProt Comments for MRPS2    MRPS2: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S2 family. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Protein type: Mitochondrial; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: mitochondrion; mitochondrial small ribosomal subunit

Molecular Function: structural constituent of ribosome

Biological Process: translation
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 Research Articles on MRPS2    1. Observational study of gene-disease association. (HuGE Navigator)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MRPS2 elisa kitPathways associated with MRPS2 elisa kit
 Reference Product  PubMed Publications
 MRPS16 elisa kit  >1 publications with MRPS2 and MRPS16
 Products by Pathway  Pathway Diagram
 Ribosome Pathway antibodies  Ribosome Pathway Diagram
 Ribosome Pathway antibodies  Ribosome Pathway Diagram
Diseases associated with MRPS2 elisa kitOrgans/Tissues associated with MRPS2 elisa kit
 Disease Name  Pubmed Publications
 Adenoma Antibodies  >1 publications with MRPS2 and Adenoma
 Thyroid Neoplasms Antibodies  >1 publications with MRPS2 and Thyroid Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Thyroid Antibodies  >1 publications with MRPS2 and Thyroid
 Kidney Antibodies  >1 publications with MRPS2 and Kidney
 Ovary Antibodies  >1 publications with MRPS2 and Ovary
 Intestine Antibodies  >1 publications with MRPS2 and Intestine
 Lung Antibodies  >1 publications with MRPS2 and Lung
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