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HMGCR elisa kit :: Human 3 Hydroxy 3 methylglutaryl CoA reductase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS742031
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

3 Hydroxy 3 methylglutaryl CoA reductase (HMGCR), ELISA Kit

★Popular Item★
 Also Known As   

Human 3 Hydroxy 3 methylglutaryl CoA reductase ELISA Kit

 Product Gene Name   

HMGCR elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Competitive ELISA Manual Insert    Competitive ELISA PDF Manual
  Sandwich ELISA Manual Insert    Sandwich ELISA PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of HMGCR. No significant cross-reactivity or interference between HMGCR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HMGCR and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HMGCR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HMGCR purchase    MBS742031 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 3 Hydroxy 3 methylglutaryl CoA reductase (HMGCR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing HMGCR, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS742031 kit is based on HMGCR antibody-HMGCR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HMGCR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HMGCR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HMGCR elisa kit

   Intended Uses||This HMGCR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HMGCR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: HMGCR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HMGCR. Standards or samples are then added to the microtiter plate wells and HMGCR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HMGCR present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HMGCR are added to each well to "sandwich” the HMGCR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HMGCR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGCR concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for HMGCR elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of HMGCR elisa kit    HMGCR elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for HMGCR. It may not necessarily be applicable to this product.
 NCBI GI #    160358778
 NCBI GeneID    15357
 NCBI Accession #    NP_032281.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_008255.2 [Other Products]
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 UniProt Secondary Accession #    Q5U4I2; G3X8U5 [Other Products]
 UniProt Related Accession #    Q01237 [Other Products]
 Molecular Weight    97,040 Da
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 NCBI Official Full Name    3-hydroxy-3-methylglutaryl-coenzyme A reductase
 NCBI Official Synonym Full Names    3-hydroxy-3-methylglutaryl-Coenzyme A reductase
 NCBI Official Symbol    Hmgcr [Similar Products]
 NCBI Official Synonym Symbols   
Red; HMG-CoAR
[Similar Products]
 NCBI Protein Information    3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMG-CoA reductase; 3-hydroxy-3-methylglutaryl-CoA reductase
 UniProt Protein Name    3-hydroxy-3-methylglutaryl-coenzyme A reductase
 UniProt Gene Name    Hmgcr [Similar Products]
 UniProt Synonym Gene Names    HMG-CoA reductase [Similar Products]
 UniProt Entry Name    HMDH_MOUSE
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 UniProt Comments for HMGCR    HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.

Protein type: Oxidoreductase; Motility/polarity/chemotaxis; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral; EC 1.1.1.34

Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein homodimerization activity; hydroxymethylglutaryl-CoA reductase (NADPH) activity; protein phosphatase 2A binding; hydroxymethylglutaryl-CoA reductase activity; oxidoreductase activity; coenzyme binding; NADP binding; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process: steroid metabolic process; cholesterol metabolic process; negative regulation of MAP kinase activity; isoprenoid biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of skeletal muscle development; cholesterol biosynthetic process; positive regulation of stress-activated MAPK cascade; coenzyme A metabolic process; embryonic development; ubiquinone metabolic process; negative regulation of vasodilation; sterol biosynthetic process; positive regulation of cell proliferation; visual learning; lipid metabolic process; steroid biosynthetic process; protein tetramerization; negative regulation of apoptosis
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 Research Articles on HMGCR    1. Increased cholesterol synthesis mediated by HMGCoA-R under inflammatory stress may be one of the mechanisms for intracellular lipid accumulation and statin resistance.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with HMGCR elisa kitDiseases associated with HMGCR elisa kit
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of Gene Expression By SREBF (SREBP) Pathway antibodies  Activation Of Gene Expression By SREBF (SREBP) Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 C5 Isoprenoid Biosynthesis, Mevalonate Pathway antibodies  C5 Isoprenoid Biosynthesis, Mevalonate Pathway Diagram
 C5 Isoprenoid Biosynthesis, Mevalonate Pathway antibodies  C5 Isoprenoid Biosynthesis, Mevalonate Pathway Diagram
 Cholesterol Biosynthesis Pathway antibodies  Cholesterol Biosynthesis Pathway Diagram
 Cholesterol Biosynthesis Pathway antibodies  Cholesterol Biosynthesis Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >57 publications with HMGCR and Cardiovascular Diseases
 Fatty Liver Antibodies  >47 publications with HMGCR and Fatty Liver
 Dyslipidemias Antibodies  >40 publications with HMGCR and Dyslipidemias
 Hypercholesterolemia Antibodies  >39 publications with HMGCR and Hypercholesterolemia
 Nervous System Diseases Antibodies  >38 publications with HMGCR and Nervous System Diseases
 Atherosclerosis Antibodies  >37 publications with HMGCR and Atherosclerosis
 Liver Diseases Antibodies  >35 publications with HMGCR and Liver Diseases
 Hyperlipidemias Antibodies  >33 publications with HMGCR and Hyperlipidemias
 Inflammation Antibodies  >26 publications with HMGCR and Inflammation
 Heart Diseases Antibodies  >25 publications with HMGCR and Heart Diseases
Organs/Tissues associated with HMGCR elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >157 publications with HMGCR and Liver
 Blood Antibodies  >147 publications with HMGCR and Blood
 Brain Antibodies  >31 publications with HMGCR and Brain
 Kidney Antibodies  >25 publications with HMGCR and Kidney
 Ovary Antibodies  >16 publications with HMGCR and Ovary
 Intestine Antibodies  >11 publications with HMGCR and Intestine
 Prostate Antibodies  >7 publications with HMGCR and Prostate
 Lung Antibodies  >6 publications with HMGCR and Lung
 Bone Antibodies  >6 publications with HMGCR and Bone
 Connective Tissue Antibodies  >6 publications with HMGCR and Connective Tissue
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