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5NT elisa kit :: Human 5 Nucleotidase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS723089
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

5 Nucleotidase (5NT), ELISA Kit

 Also Known As   

Human 5 Nucleotidase ELISA Kit

 Product Gene Name   

5NT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 211800
 3D Structure    ModBase 3D Structure for P21589
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 Species Reactivity    Human
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This 5NT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 5NT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of 5NT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for 5NT purchase    MBS723089 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5 Nucleotidase (5NT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing 5NT, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS723089 kit is based on 5NT antibody-5NT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 5NT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 5NT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for 5NT elisa kit

   Principle of the Assay: 5NT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-5NT antibody and an 5NT-HRP conjugate. The assay sample and buffer are incubated together with 5NT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 5NT concentration since 5NT from samples and 5NT-HRP conjugate compete for the anti-5NT antibody binding site. Since the number of sites is limited, as more sites are occupied by 5NT from the sample, fewer sites are left to bind 5NT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 5NT concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for 5NT elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve (for reference only) of 5NT elisa kit    5NT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS723089. Click to request current manual
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NCBI/Uniprot data below describe general gene information for 5NT. It may not necessarily be applicable to this product.
 NCBI GI #    23897
 NCBI GeneID    4907
 NCBI Accession #    CAA39271.1 [Other Products]
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 UniProt Primary Accession #    P21589 [Other Products]
 UniProt Secondary Accession #    O75520; Q5W116; B3KQI8 [Other Products]
 UniProt Related Accession #    P21589 [Other Products]
 Molecular Weight    57,949 Da
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 NCBI Official Full Name    5'-nucleotidase
 NCBI Official Synonym Full Names    5'-nucleotidase, ecto (CD73)
 NCBI Official Symbol    NT5E [Similar Products]
 NCBI Official Synonym Symbols   
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
[Similar Products]
 NCBI Protein Information    5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase
 UniProt Protein Name    5'-nucleotidase
 UniProt Synonym Protein Names   
Ecto-5'-nucleotidase; CD_antigen: CD73
 UniProt Gene Name    NT5E [Similar Products]
 UniProt Synonym Gene Names    NT5; NTE; 5'-NT [Similar Products]
 UniProt Entry Name    5NTD_HUMAN
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 NCBI Summary for 5NT    The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
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 UniProt Comments for 5NT    NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Phosphatase (non-protein); Membrane protein, GPI anchor; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of inflammatory response; adenosine biosynthetic process; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries
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 Research Articles on 5NT    1. Isoflurane causes TGF-beta1-dependent induction of renal tubular CD73 and adenosine generation to protect against renal ischemia and reperfusion injury.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with 5NT elisa kitPathways associated with 5NT elisa kit
 Reference Product  PubMed Publications
 ENPP1 elisa kit  >4 publications with 5NT and ENPP1
 ADA elisa kit  >3 publications with 5NT and ADA
 ENTPD1 elisa kit  >2 publications with 5NT and ENTPD1
 Products by Pathway  Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 NAD Salvage Pathway II antibodies  NAD Salvage Pathway II Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Purine Catabolism Pathway antibodies  Purine Catabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
Diseases associated with 5NT elisa kitOrgans/Tissues associated with 5NT elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >17 publications with 5NT and Inflammation
 Nervous System Diseases Antibodies  >9 publications with 5NT and Nervous System Diseases
 Necrosis Antibodies  >9 publications with 5NT and Necrosis
 Breast Neoplasms Antibodies  >9 publications with 5NT and Breast Neoplasms
 Disease Models, Animal Antibodies  >7 publications with 5NT and Disease Models, Animal
 Pain Antibodies  >7 publications with 5NT and Pain
 Brain Diseases Antibodies  >5 publications with 5NT and Brain Diseases
 Kidney Diseases Antibodies  >4 publications with 5NT and Kidney Diseases
 Heart Diseases Antibodies  >4 publications with 5NT and Heart Diseases
 Acute Kidney Injury Antibodies  >3 publications with 5NT and Acute Kidney Injury
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >31 publications with 5NT and Blood
 Vascular Antibodies  >19 publications with 5NT and Vascular
 Bone Antibodies  >13 publications with 5NT and Bone
 Bone Marrow Antibodies  >8 publications with 5NT and Bone Marrow
 Muscle Antibodies  >8 publications with 5NT and Muscle
 Mouth Antibodies  >8 publications with 5NT and Mouth
 Brain Antibodies  >8 publications with 5NT and Brain
 Kidney Antibodies  >6 publications with 5NT and Kidney
 Ganglia Antibodies  >6 publications with 5NT and Ganglia
 Embryonic Tissue Antibodies  >5 publications with 5NT and Embryonic Tissue
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