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PFKFB3 elisa kit :: Human 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS911784
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3), ELISA Kit

 Also Known As   

Human 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3, PFKFB3 ELISA Kit

 Product Synonym Names    Human 6-phosphofructo-2-kinase/fructose-2; 6-biphosphatase 3 (PFKFB3) ELISA kit; RP11-298K24.3; FLJ37326; IPFK2; PFK2; 6-phosphofructo-2-kinase/ fructose-2; 6-bisphosphatase; 6-phosphofructo-2-kinase/fructose-2; 6-bisphosphatase; fructose-6-phosphate; 2-kinase/fructose-2; 6-bisphosp; 6-phosphofructo-2-kinase/fructose-2; 6-biphosphatase 3
 Product Gene Name   

PFKFB3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS911784 Testing Data    Testing Data PDF
 OMIM    605319
 3D Structure    ModBase 3D Structure for Q16875
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human PFKFB3. No significant cross-reactivity or interference between human PFKFB3 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates.
 Assay Type    Sandwich (Quantitative)
 Detection Range    47 pg/ml -3000 pg/ml.
 Sensitivity    11.75 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PFKFB3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PFKFB3 purchase    MBS911784 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 47 pg/ml -3000 pg/ml in biological research samples containing PFKFB3, with an estimated sensitivity of 11.75 pg/ml. The ELISA analytical biochemical technique of the MBS911784 kit is based on PFKFB3 antibody-PFKFB3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PFKFB3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PFKFB3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PFKFB3 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PFKFB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PFKFB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFKFB3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFKFB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of PFKFB3 elisa kit    PFKFB3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS911784. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PFKFB3. It may not necessarily be applicable to this product.
 NCBI GI #    224282149
 NCBI GeneID    5209
 NCBI Accession #    NP_001138915.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001145443.1 [Other Products]
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 UniProt Primary Accession #    Q16875 [Other Products]
 UniProt Secondary Accession #    O43622; O75902; Q5VX15; Q5VX18; Q5VX19; B7Z955 [Other Products]
 UniProt Related Accession #    Q16875 [Other Products]
 Molecular Weight    59,609 Da
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 NCBI Official Full Name    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 2
 NCBI Official Synonym Full Names    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
 NCBI Official Symbol    PFKFB3 [Similar Products]
 NCBI Official Synonym Symbols   
PFK2; IPFK2
[Similar Products]
 NCBI Protein Information    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; iPFK-2; PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase 3; renal carcinoma antigen NY-REN-56; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase; 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase; fructose-6-phosphate,2-kinase/fructose-2, 6-bisphosphatase; inducible 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
 UniProt Protein Name    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
 UniProt Synonym Protein Names   
6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; Renal carcinoma antigen NY-REN-56; iPFK-2Including the following 2 domains:6-phosphofructo-2-kinase (EC:2.7.1.105); Fructose-2,6-bisphosphatase (EC:3.1.3.46)
 Protein Family    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
 UniProt Gene Name    PFKFB3 [Similar Products]
 UniProt Synonym Gene Names    6PF-2-K/Fru-2,6-P2ase 3; PFK/FBPase 3 [Similar Products]
 UniProt Entry Name    F263_HUMAN
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 UniProt Comments for PFKFB3    PFKFB3: Synthesis and degradation of fructose 2,6-bisphosphate. Ubiquitous. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Carbohydrate Metabolism - fructose and mannose; Kinase, other; Phosphatase (non-protein); EC 3.1.3.46; EC 2.7.1.105

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: nucleoplasm; cytosol

Molecular Function: fructose-2,6-bisphosphate 2-phosphatase activity; 6-phosphofructo-2-kinase activity; ATP binding

Biological Process: glycolysis; dephosphorylation; carbohydrate phosphorylation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; brain development; fructose 2,6-bisphosphate metabolic process; fructose metabolic process
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 Research Articles on PFKFB3    1. PFKFB3 is a promising target for the reduction of endothelial glycolysis and its related pathological angiogenesis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PFKFB3 elisa kitDiseases associated with PFKFB3 elisa kit
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Carbohydrates Pathway antibodies  Metabolism Of Carbohydrates Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >22 publications with PFKFB3 and Neoplasms
 Breast Neoplasms Antibodies  >6 publications with PFKFB3 and Breast Neoplasms
 Skin Diseases Antibodies  >5 publications with PFKFB3 and Skin Diseases
 Inflammation Antibodies  >4 publications with PFKFB3 and Inflammation
 Insulin Resistance Antibodies  >4 publications with PFKFB3 and Insulin Resistance
 Anoxia Antibodies  >4 publications with PFKFB3 and Anoxia
 Adenocarcinoma Antibodies  >4 publications with PFKFB3 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >4 publications with PFKFB3 and Cell Transformation, Neoplastic
 Brain Diseases Antibodies  >3 publications with PFKFB3 and Brain Diseases
 Carcinoma Antibodies  >3 publications with PFKFB3 and Carcinoma
Organs/Tissues associated with PFKFB3 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >12 publications with PFKFB3 and Brain
 Muscle Antibodies  >7 publications with PFKFB3 and Muscle
 Testis Antibodies  >6 publications with PFKFB3 and Testis
 Kidney Antibodies  >5 publications with PFKFB3 and Kidney
 Blood Antibodies  >5 publications with PFKFB3 and Blood
 Adipose Tissue Antibodies  >4 publications with PFKFB3 and Adipose Tissue
 Vascular Antibodies  >4 publications with PFKFB3 and Vascular
 Lung Antibodies  >2 publications with PFKFB3 and Lung
 Pancreas Antibodies  >2 publications with PFKFB3 and Pancreas
 Placenta Antibodies  >2 publications with PFKFB3 and Placenta
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