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RPL8 elisa kit :: Human 60S ribosomal protein L8 (RPL8) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7219850
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
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 Product Name   

60S ribosomal protein L8 (RPL8), ELISA Kit

★Popular Item★
 Also Known As   

Human 60S ribosomal protein L8 (RPL8) ELISA Kit

 Product Gene Name   

RPL8 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    604177
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of RPL8. No significant cross-reactivity or interference between RPL8 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPL8 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RPL8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RPL8 purchase    MBS7219850 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 60S ribosomal protein L8 (RPL8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing RPL8, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7219850 kit is based on RPL8 antibody-RPL8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RPL8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RPL8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RPL8 elisa kit

   Intended Uses: This RPL8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RPL8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||RPL8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPL8 antibody and an RPL8-HRP conjugate. The assay sample and buffer are incubated together with RPL8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPL8 concentration since RPL8 from samples and RPL8-HRP conjugate compete for the anti-RPL8 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPL8 from the sample, fewer sites are left to bind RPL8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPL8 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for RPL8 elisa kit    Epigenetics and Nuclear Signaling
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 Typical Testing Data/Standard Curve of RPL8 elisa kit    RPL8 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS7219850. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RPL8. It may not necessarily be applicable to this product.
 NCBI GI #    15431306
 NCBI GeneID    6132
 NCBI Accession #    NP_150644.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_033301.1 [Other Products]
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 UniProt Secondary Accession #    P25120; Q567Q7; Q969V7; Q9BWQ9; A8K094; D3DWN2 [Other Products]
 UniProt Related Accession #    P62917 [Other Products]
 Molecular Weight    28,025 Da
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 NCBI Official Full Name    60S ribosomal protein L8
 NCBI Official Synonym Full Names    ribosomal protein L8
 NCBI Official Symbol    RPL8 [Similar Products]
 NCBI Official Synonym Symbols   
L8
[Similar Products]
 NCBI Protein Information    60S ribosomal protein L8
 UniProt Protein Name    60S ribosomal protein L8
 Protein Family    60S ribosomal protein
 UniProt Gene Name    RPL8 [Similar Products]
 UniProt Entry Name    RL8_HUMAN
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 NCBI Summary for RPL8    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
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 UniProt Comments for RPL8    RPL8: a ribosomal protein of the L2P family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit. Associates with 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface.

Protein type: Ribosomal; Translation; RNA-binding

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: focal adhesion; membrane; cytoplasm; nucleolus; cytosol

Molecular Function: rRNA binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; translational termination; viral infectious cycle
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 Research Articles on RPL8    1. gain of RPL8 is associated with good response to chemotherapy in conventional osteosarcomas.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RPL8 elisa kitPathways associated with RPL8 elisa kit
 Reference Product  PubMed Publications
 RPS5 elisa kit  >3 publications with RPL8 and RPS5
 RPL11 elisa kit  >3 publications with RPL8 and RPL11
 RPS13 elisa kit  >2 publications with RPL8 and RPS13
 RPL35 elisa kit  >2 publications with RPL8 and RPL35
 RPS14 elisa kit  >1 publications with RPL8 and RPS14
 Products by Pathway  Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Cytoplasmic Ribosomal Proteins Pathway antibodies  Cytoplasmic Ribosomal Proteins Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Eukaryotic Translation Elongation Pathway antibodies  Eukaryotic Translation Elongation Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 Eukaryotic Translation Termination Pathway antibodies  Eukaryotic Translation Termination Pathway Diagram
 Formation Of A Pool Of Free 40S Subunits Pathway antibodies  Formation Of A Pool Of Free 40S Subunits Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Influenza Infection Pathway antibodies  Influenza Infection Pathway Diagram
Diseases associated with RPL8 elisa kitOrgans/Tissues associated with RPL8 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >2 publications with RPL8 and Carcinoma
 Necrosis Antibodies  >2 publications with RPL8 and Necrosis
 Anemia Antibodies  >1 publications with RPL8 and Anemia
 Abnormalities, Multiple Antibodies  >1 publications with RPL8 and Abnormalities, Multiple
 Liver Diseases Antibodies  >1 publications with RPL8 and Liver Diseases
 Adenocarcinoma Antibodies  >1 publications with RPL8 and Adenocarcinoma
 Carcinoma, Hepatocellular Antibodies  >1 publications with RPL8 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >1 publications with RPL8 and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >4 publications with RPL8 and Liver
 Blood Antibodies  >3 publications with RPL8 and Blood
 Salivary Gland Antibodies  >2 publications with RPL8 and Salivary Gland
 Bone Antibodies  >2 publications with RPL8 and Bone
 Muscle Antibodies  >2 publications with RPL8 and Muscle
 Ovary Antibodies  >1 publications with RPL8 and Ovary
 Kidney Antibodies  >1 publications with RPL8 and Kidney
 Embryonic Tissue Antibodies  >1 publications with RPL8 and Embryonic Tissue
 Vascular Antibodies  >1 publications with RPL8 and Vascular
 Parathyroid Antibodies  >1 publications with RPL8 and Parathyroid
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