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DHCR7 elisa kit :: Human 7-Dehydrocholesterol Reductase (DHCR7) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2019907 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

7-Dehydrocholesterol Reductase (DHCR7), ELISA Kit

 Also Known As   

7-Dehydrocholesterol Reductase (DHCR7) ELISA Kit

 Product Synonym Names    DHCR-7; SLOS; D7SR; Smith-Lemli-Opitz Syndrome; Putative sterol reductase SR-2; Sterol Delta(7)-reductase
 Product Gene Name   

DHCR7 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    270400
 3D Structure    ModBase 3D Structure for Q9UBM7
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of 7-Dehydrocholesterol Reductase (DHCR7).
No significant cross-reactivity or interference between 7-Dehydrocholesterol Reductase (DHCR7) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.055ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level 7-Dehydrocholesterol Reductase (DHCR7) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level 7-Dehydrocholesterol Reductase (DHCR7) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DHCR7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DHCR7 purchase    MBS2019907 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 7-Dehydrocholesterol Reductase (DHCR7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing DHCR7, with an estimated sensitivity of < 0.055ng/mL. The ELISA analytical biochemical technique of the MBS2019907 kit is based on DHCR7 antibody-DHCR7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DHCR7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DHCR7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DHCR7 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 7-Dehydrocholesterol Reductase (DHCR7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 7-Dehydrocholesterol Reductase (DHCR7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 7-Dehydrocholesterol Reductase (DHCR7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 7-Dehydrocholesterol Reductase (DHCR7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for DHCR7 elisa kit    Enzyme & Kinase
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 Typical Testing Data/Standard Curve (for reference only) of DHCR7 elisa kit    DHCR7 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2019907. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DHCR7. It may not necessarily be applicable to this product.
 NCBI GI #    255308875
 NCBI GeneID    1717
 NCBI Accession #    NP_001157289.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001163817.1 [Other Products]
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 UniProt Primary Accession #    Q9UBM7 [Other Products]
 UniProt Secondary Accession #    O60492; O60717; B2R6Z2 [Other Products]
 UniProt Related Accession #    Q9UBM7 [Other Products]
 Molecular Weight    54,489 Da
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 NCBI Official Full Name    7-dehydrocholesterol reductase
 NCBI Official Synonym Full Names    7-dehydrocholesterol reductase
 NCBI Official Symbol    DHCR7 [Similar Products]
 NCBI Official Synonym Symbols   
SLOS
[Similar Products]
 NCBI Protein Information    7-dehydrocholesterol reductase
 UniProt Protein Name    7-dehydrocholesterol reductase
 UniProt Synonym Protein Names   
Putative sterol reductase SR-2; Sterol Delta(7)-reductase
 Protein Family    Probable 7-dehydrocholesterol reductase
 UniProt Gene Name    DHCR7 [Similar Products]
 UniProt Synonym Gene Names    D7SR; 7-DHC reductase [Similar Products]
 UniProt Entry Name    DHCR7_HUMAN
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 NCBI Summary for DHCR7    This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009]
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 UniProt Comments for DHCR7    DHCR7: Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC). Defects in DHCR7 are the cause of Smith-Lemli-Opitz syndrome (SLOS); also known as SLO syndrome or RSH syndrome. SLOS is an autosomal recessive frequent inborn disorder of sterol metabolism with characteristic congenital malformations and dysmorphias. All patients suffer from mental retardation. Children with SLOS have elevated serum 7-dehydrocholesterol (7- DHC) levels and low serum cholesterol levels. SLOS occurs in relatively high frequency: approximately 1 in 20,000 to 30,000 births in populations of northern and central European background. Historically, a clinical distinction often was made between classic ('type I') SLOS and the more severely affected ('type II') patients. There is, in reality, a clinical and biochemical continuum from mild to severe SLOS. Belongs to the ERG4/ERG24 family.

Protein type: EC 1.3.1.21; Lipid Metabolism - steroid biosynthesis; Membrane protein, multi-pass; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: nuclear outer membrane; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: 7-dehydrocholesterol reductase activity

Biological Process: blood vessel development; regulation of cholesterol biosynthetic process; multicellular organism growth; cell differentiation; cholesterol biosynthetic process; post-embryonic development; regulation of cell proliferation; lung development

Disease: Smith-lemli-opitz Syndrome
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 Research Articles on DHCR7    1. In this mendelian randomisation study, we generated an allele score (25[OH]D synthesis score) based on variants of genes that affect 25(OH)D synthesis or substrate availability (CYP2R1 and DHCR7)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DHCR7 elisa kitPathways associated with DHCR7 elisa kit
 Reference Product  PubMed Publications
 DHCR24 elisa kit  >11 publications with DHCR7 and DHCR24
 SQLE elisa kit  >4 publications with DHCR7 and SQLE
 SP1 elisa kit  >2 publications with DHCR7 and SP1
 SC5DL elisa kit  >2 publications with DHCR7 and SC5DL
 SREBF2 elisa kit  >1 publications with DHCR7 and SREBF2
 Products by Pathway  Pathway Diagram
 Activation Of Gene Expression By SREBF (SREBP) Pathway antibodies  Activation Of Gene Expression By SREBF (SREBP) Pathway Diagram
 Cholesterol Biosynthesis Pathway antibodies  Cholesterol Biosynthesis Pathway Diagram
 Cholesterol Biosynthesis Pathway antibodies  Cholesterol Biosynthesis Pathway Diagram
 Cholesterol Biosynthesis, Squalene 2,3-epoxide => Cholesterol Pathway antibodies  Cholesterol Biosynthesis, Squalene 2,3-epoxide => Cholesterol Pathway Diagram
 Cholesterol Biosynthesis, Squalene 2,3-epoxide => Cholesterol Pathway antibodies  Cholesterol Biosynthesis, Squalene 2,3-epoxide => Cholesterol Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Regulation Of Cholesterol Biosynthesis By SREBP (SREBF) Pathway antibodies  Regulation Of Cholesterol Biosynthesis By SREBP (SREBF) Pathway Diagram
 Steroid Biosynthesis Pathway antibodies  Steroid Biosynthesis Pathway Diagram
Diseases associated with DHCR7 elisa kitOrgans/Tissues associated with DHCR7 elisa kit
 Disease Name  Pubmed Publications
 Smith-Lemli-Opitz Syndrome Antibodies  >133 publications with DHCR7 and Smith-Lemli-Opitz Syndrome
 Dyslipidemias Antibodies  >117 publications with DHCR7 and Dyslipidemias
 Disease Models, Animal Antibodies  >24 publications with DHCR7 and Disease Models, Animal
 Fetal Death Antibodies  >9 publications with DHCR7 and Fetal Death
 Liver Diseases Antibodies  >7 publications with DHCR7 and Liver Diseases
 Inflammation Antibodies  >5 publications with DHCR7 and Inflammation
 Fatty Liver Antibodies  >4 publications with DHCR7 and Fatty Liver
 Fibrosis Antibodies  >4 publications with DHCR7 and Fibrosis
 Heart Diseases Antibodies  >4 publications with DHCR7 and Heart Diseases
 Atherosclerosis Antibodies  >3 publications with DHCR7 and Atherosclerosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >71 publications with DHCR7 and Blood
 Brain Antibodies  >36 publications with DHCR7 and Brain
 Liver Antibodies  >34 publications with DHCR7 and Liver
 Embryonic Tissue Antibodies  >16 publications with DHCR7 and Embryonic Tissue
 Heart Antibodies  >14 publications with DHCR7 and Heart
 Bone Antibodies  >11 publications with DHCR7 and Bone
 Nerve Antibodies  >10 publications with DHCR7 and Nerve
 Skin Antibodies  >10 publications with DHCR7 and Skin
 Lung Antibodies  >9 publications with DHCR7 and Lung
 Eye Antibodies  >6 publications with DHCR7 and Eye
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