Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow ADAM10 rightarrow Human ADAM10  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • WEE1 homolog (S. pombe) ELISA Kit
 • HIV-2 PCR Kit
 • GDNF (GDNF ) ELISA Kit
 • keratin 83 (KRT83) ELISA Kit
 • PSA Antibody
 • LIMP2-SCARB2 Antibody
 • CD2 Antibody
 • IL-3Rbeta Antibody
 • AKT1-3 Antibody
 • 1,5-Anhydroglucitol (1,5-AG) ELISA Kit
 • PK ELISA Kit
 • Steroid 21-hydroxylase (CYP21A2) Recombinant Protein
 • LZM ELISA Kit
 • APOA1 - Apolipoprotein A 1 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

ADAM10 elisa kit :: Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021378 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $255 +1 FREE 8GB USB
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $610 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,470 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,445 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

A Disintegrin And Metalloprotease 10 (ADAM10), ELISA Kit

★Popular Item★
 Also Known As   

A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

 Product Synonym Names    CD156-C; CD156c; MADM; Kuz; CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease
 Product Gene Name   

ADAM10 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    277600
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 10 (ADAM10).
No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 10 (ADAM10) and analogues was observed.
Section Bar
 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    78-5,000pg/mL
 Sensitivity    < 28pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
Section Bar
 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADAM10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADAM10 purchase    MBS2021378 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78-5,000pg/mL in biological research samples containing ADAM10, with an estimated sensitivity of < 28pg/mL. The ELISA analytical biochemical technique of the MBS2021378 kit is based on ADAM10 antibody-ADAM10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for ADAM10 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 10 (ADAM10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 10 (ADAM10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for ADAM10 elisa kit    Enzyme & Kinase; Tumor immunity; Infection immunity; Cardiovascular biology
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of ADAM10 elisa kit    ADAM10 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS2021378. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ADAM10. It may not necessarily be applicable to this product.
 NCBI GI #    537544566
 NCBI GeneID    81794
 NCBI Accession #    NP_112219.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_030957.3 [Other Products]
Section Bar
 UniProt Secondary Accession #    M0QZE4 [Other Products]
 UniProt Related Accession #    Q9H324 [Other Products]
 Molecular Weight    62,530 Da
Table BarTOPTable Bar
 NCBI Official Full Name    A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform 1 preproprotein
 NCBI Official Synonym Full Names    ADAM metallopeptidase with thrombospondin type 1 motif, 10
 NCBI Official Symbol    ADAMTS10 [Similar Products]
 NCBI Official Synonym Symbols   
WMS; WMS1; ADAM-TS10; ADAMTS-10
[Similar Products]
 NCBI Protein Information    A disintegrin and metalloproteinase with thrombospondin motifs 10
 UniProt Protein Name    A disintegrin and metalloproteinase with thrombospondin motifs 10
 Protein Family    Disintegrin and metalloproteinase domain-containing protein
 UniProt Gene Name    ADAMTS10 [Similar Products]
 UniProt Synonym Gene Names    ADAM-TS 10; ADAM-TS10; ADAMTS-10 [Similar Products]
 UniProt Entry Name    ATS10_HUMAN
Section Bar
 NCBI Summary for ADAM10    This gene belongs to the ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 motifs) family of zinc-dependent proteases. ADAMTS proteases are complex secreted enzymes containing a prometalloprotease domain of the reprolysin type attached to an ancillary domain with a highly conserved structure that includes at least one thrombospondin type 1 repeat. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. The product of this gene plays a major role in growth and in skin, lens, and heart development. It is also a candidate gene for autosomal recessive Weill-Marchesani syndrome. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for ADAM10    ADAMTS10: Metalloprotease that participate in microfibrils assembly. Microfibrils are extracellular matrix components occurring independently or along with elastin in the formation of elastic tissues. Defects in ADAMTS10 are the cause of Weill-Marchesani syndrome 1 (WMS1). WMS1 is a rare connective tissue disorder characterized by short stature, brachydactyly, joint stiffness, and eye abnormalities including microspherophakia, ectopia lentis, severe myopia and glaucoma.

Protein type: Motility/polarity/chemotaxis; Protease; Secreted, signal peptide; EC 3.4.24.-; Secreted

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular matrix; microfibril

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity

Biological Process: proteolysis

Disease: Weill-marchesani Syndrome 1
Table BarTOPTable Bar
 Research Articles on ADAM10    1. These findings support the Gly661Arg mutation of ADAMTS10 as the likely cause of POAG in beagles.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with ADAM10 elisa kitPathways associated with ADAM10 elisa kit
 Reference Product  PubMed Publications
 FBN1 elisa kit  >11 publications with ADAM10 and FBN1
 LTBP1 elisa kit  >1 publications with ADAM10 and LTBP1
 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 O-glycosylation Of TSR Domain-containing Proteins Pathway antibodies  O-glycosylation Of TSR Domain-containing Proteins Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
Diseases associated with ADAM10 elisa kitOrgans/Tissues associated with ADAM10 elisa kit
 Disease Name  Pubmed Publications
 Weill-Marchesani Syndrome Antibodies  >16 publications with ADAM10 and Weill-Marchesani Syndrome
 Pregnancy Complications Antibodies  >1 publications with ADAM10 and Pregnancy Complications
 Kidney Diseases Antibodies  >1 publications with ADAM10 and Kidney Diseases
 Heart Diseases Antibodies  >1 publications with ADAM10 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >11 publications with ADAM10 and Eye
 Skin Antibodies  >3 publications with ADAM10 and Skin
 Blood Antibodies  >3 publications with ADAM10 and Blood
 Nerve Antibodies  >2 publications with ADAM10 and Nerve
 Heart Antibodies  >2 publications with ADAM10 and Heart
 Kidney Antibodies  >2 publications with ADAM10 and Kidney
 Lung Antibodies  >1 publications with ADAM10 and Lung
 Embryonic Tissue Antibodies  >1 publications with ADAM10 and Embryonic Tissue
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions