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ADAM15 elisa kit :: Human A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2022132 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

A Disintegrin And Metalloprotease 15 (ADAM15), ELISA Kit

★Popular Item★
 Also Known As   

A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit

 Product Synonym Names    MDC15; Metargidin; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; Metalloprotease RGD disintegrin protein
 Product Gene Name   

ADAM15 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    607509
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 15 (ADAM15).
No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 15 (ADAM15) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.055ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level A Disintegrin And Metalloprotease 15 (ADAM15) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level A Disintegrin And Metalloprotease 15 (ADAM15) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADAM15 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADAM15 purchase    MBS2022132 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing ADAM15, with an estimated sensitivity of < 0.055ng/mL. The ELISA analytical biochemical technique of the MBS2022132 kit is based on ADAM15 antibody-ADAM15 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM15 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM15. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADAM15 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 15 (ADAM15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 15 (ADAM15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 15 (ADAM15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 15 (ADAM15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for ADAM15 elisa kit    Enzyme & Kinase
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 Typical Testing Data/Standard Curve (for reference only) of ADAM15 elisa kit    ADAM15 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2022132. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADAM15. It may not necessarily be applicable to this product.
 NCBI GI #    21265058
 NCBI GeneID    170689
 NCBI Accession #    NP_620686.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_139055.2 [Other Products]
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 UniProt Secondary Accession #    Q32MI6 [Other Products]
 UniProt Related Accession #    Q8TE58 [Other Products]
 Molecular Weight    103,287 Da
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 NCBI Official Full Name    A disintegrin and metalloproteinase with thrombospondin motifs 15 preproprotein
 NCBI Official Synonym Full Names    ADAM metallopeptidase with thrombospondin type 1 motif, 15
 NCBI Official Symbol    ADAMTS15 [Similar Products]
 NCBI Protein Information    A disintegrin and metalloproteinase with thrombospondin motifs 15
 UniProt Protein Name    A disintegrin and metalloproteinase with thrombospondin motifs 15
 Protein Family    Disintegrin and metalloproteinase domain-containing protein
 UniProt Gene Name    ADAMTS15 [Similar Products]
 UniProt Synonym Gene Names    ADAM-TS 15; ADAM-TS15; ADAMTS-15 [Similar Products]
 UniProt Entry Name    ATS15_HUMAN
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 NCBI Summary for ADAM15    This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene has a high sequence similarity to the proteins encoded by ADAMTS1 and ADAMTS8. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ADAM15    ADAMTS15:

Protein type: Motility/polarity/chemotaxis; Secreted; Protease; Extracellular matrix; Secreted, signal peptide; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 11q25

Cellular Component: proteinaceous extracellular matrix

Molecular Function: zinc ion binding; metalloendopeptidase activity

Biological Process: proteolysis
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 Research Articles on ADAM15    1. ADAMTS15 is a candidate gene for intracranial aneurysms
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADAM15 elisa kitPathways associated with ADAM15 elisa kit
 Reference Product  PubMed Publications
 FURIN elisa kit  >1 publications with ADAM15 and FURIN
 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 O-glycosylation Of TSR Domain-containing Proteins Pathway antibodies  O-glycosylation Of TSR Domain-containing Proteins Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
Diseases associated with ADAM15 elisa kitOrgans/Tissues associated with ADAM15 elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >3 publications with ADAM15 and Breast Neoplasms
 Melanoma Antibodies  >2 publications with ADAM15 and Melanoma
 Hyperplasia Antibodies  >1 publications with ADAM15 and Hyperplasia
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >3 publications with ADAM15 and Prostate
 Brain Antibodies  >3 publications with ADAM15 and Brain
 Lung Antibodies  >2 publications with ADAM15 and Lung
 Heart Antibodies  >2 publications with ADAM15 and Heart
 Kidney Antibodies  >2 publications with ADAM15 and Kidney
 Embryonic Tissue Antibodies  >2 publications with ADAM15 and Embryonic Tissue
 Connective Tissue Antibodies  >1 publications with ADAM15 and Connective Tissue
 Vascular Antibodies  >1 publications with ADAM15 and Vascular
 Ovary Antibodies  >1 publications with ADAM15 and Ovary
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