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ADAM elisa kit :: Human A Disintegrin And Metalloprotease ELISA Kit

Scan QR to view Datasheet Catalog #    MBS738650
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

A Disintegrin And Metalloprotease (ADAM), ELISA Kit

★Popular Item★
 Also Known As   

Human A Disintegrin And Metalloprotease ELISA Kit

 Product Gene Name   

ADAM elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ADAM. No significant cross-reactivity or interference between ADAM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAM and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This ADAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADAM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADAM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADAM purchase    MBS738650 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin And Metalloprotease (ADAM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing ADAM, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS738650 kit is based on ADAM antibody-ADAM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADAM elisa kit

   Principle of the assay: ADAM ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ADAM. Standards or samples are then added to the microtiter plate wells and ADAM if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADAM present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADAM are added to each well to "sandwich" the ADAM immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ADAM and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ADAM elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of ADAM elisa kit    ADAM elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS738650. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADAM. It may not necessarily be applicable to this product.
 NCBI GI #    133778949
 NCBI GeneID    108153
 NCBI Accession #    NP_001003911.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001003911.2 [Other Products]
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 UniProt Secondary Accession #    B2RUI8; E9QMY0 [Other Products]
 UniProt Related Accession #    Q68SA9 [Other Products]
 Molecular Weight    177,712 Da
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 NCBI Official Full Name    A disintegrin and metalloproteinase with thrombospondin motifs 7
 NCBI Official Synonym Full Names    a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 7
 NCBI Official Symbol    Adamts7 [Similar Products]
 NCBI Official Synonym Symbols   
ADAM-TS7; ADAMTS7B
[Similar Products]
 NCBI Protein Information    A disintegrin and metalloproteinase with thrombospondin motifs 7; COMPase; ADAMTS-7; ADAM-TS 7; metalloprotease; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 7
 UniProt Protein Name    A disintegrin and metalloproteinase with thrombospondin motifs 7
 UniProt Synonym Protein Names   
COMPase
 Protein Family    Eukaryotic translation initiation factor
 UniProt Gene Name    Adamts7 [Similar Products]
 UniProt Synonym Gene Names    ADAM-TS 7; ADAM-TS7; ADAMTS-7 [Similar Products]
 UniProt Entry Name    ATS7_MOUSE
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 UniProt Comments for ADAM    ADAMTS7: Metalloprotease that may play a role in the degradation of COMP.

Protein type: EC 3.4.24.-; Secreted; Protease; Cell surface; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; cell surface; extracellular region

Molecular Function: peptidase activity; metallopeptidase activity; hydrolase activity; zinc ion binding; metalloendopeptidase activity; metal ion binding

Biological Process: proteolysis involved in cellular protein catabolic process; negative regulation of chondrocyte differentiation; proteolysis
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 Research Articles on ADAM    1. ADAMTS-7 and TNF-alpha form a positive feedback loop in the regulation of cartilage degradation and osteoarthritis progression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ADAM elisa kitDiseases associated with ADAM elisa kit
 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 O-glycosylation Of TSR Domain-containing Proteins Pathway antibodies  O-glycosylation Of TSR Domain-containing Proteins Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >11 publications with ADAM and Cardiovascular Diseases
 Coronary Artery Disease Antibodies  >9 publications with ADAM and Coronary Artery Disease
 Arteriosclerosis Antibodies  >8 publications with ADAM and Arteriosclerosis
 Necrosis Antibodies  >6 publications with ADAM and Necrosis
 Disease Models, Animal Antibodies  >5 publications with ADAM and Disease Models, Animal
 Myocardial Infarction Antibodies  >4 publications with ADAM and Myocardial Infarction
 Inflammation Antibodies  >3 publications with ADAM and Inflammation
 Kidney Diseases Antibodies  >1 publications with ADAM and Kidney Diseases
 Hypertension Antibodies  >1 publications with ADAM and Hypertension
 Congenital Abnormalities Antibodies  >1 publications with ADAM and Congenital Abnormalities
Organs/Tissues associated with ADAM elisa kit
 Organ/Tissue Name  Pubmed Publications
 Joint Antibodies  >5 publications with ADAM and Joint
 Heart Antibodies  >4 publications with ADAM and Heart
 Eye Antibodies  >1 publications with ADAM and Eye
 Embryonic Tissue Antibodies  >1 publications with ADAM and Embryonic Tissue
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