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| Product Name
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ACD, ELISA Kit ★Popular Item★
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| Also Known As
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Human ACD (Adrenocortical Dysplasia Homolog) ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| OMIM
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609377
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| 3D Structure
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ModBase 3D Structure for Q96AP0
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| Species Reactivity
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Human
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| Specificity
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This kit recognizes natural and recombinantHumanACD. No significant cross-reactivity or interference between HumanACD and analogues was observed.
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| Samples
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Serum, plasma and other biological fluids
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| Assay Type
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Quantitative Sandwich
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| Detection Range
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31.25-2000pg/mL
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| Sensitivity
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18.75pg/mL
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| Preparation and Storage
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Store at 4 degree C.
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of ACD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for ACD purchase
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MBS2500388 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ACD, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000pg/mL in biological research samples containing ACD, with an estimated sensitivity of 18.75pg/mL. The ELISA analytical biochemical technique of the MBS2500388 kit is based on ACD antibody-ACD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ACD elisa kit
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Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanACDconcentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ACD. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ACDand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ACD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ACD.You can calculate the concentration of ACDin the samples by comparing the OD of the samples to the standard curve.
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| Typical Testing Data/Standard Curve (for reference only) of ACD elisa kit
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| Sample Manual Insert of MBS2500388. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for ACD. It may not necessarily be applicable to this product. |
| NCBI GI #
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130979214
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| NCBI GeneID
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65057
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| NCBI Accession #
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NP_001075956.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_001082487.1
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[Other Products]
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| UniProt Primary Accession #
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Q96AP0
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[Other Products]
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| UniProt Secondary Accession #
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Q562H5; Q9H8F9
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[Other Products]
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| UniProt Related Accession #
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Q96AP0
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[Other Products]
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| Molecular Weight
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57,393 Da
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| NCBI Official Full Name
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adrenocortical dysplasia protein homolog isoform 3
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| NCBI Official Synonym Full Names
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adrenocortical dysplasia homolog (mouse)
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| NCBI Official Symbol
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ACD
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[Similar Products]
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| NCBI Official Synonym Symbols
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PIP1; PTOP; TPP1; TINT1
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[Similar Products]
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| NCBI Protein Information
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adrenocortical dysplasia protein homolog; POT1 and TIN2 organizing protein; POT1 and TIN2-interacting protein; TIN2 interacting protein 1
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| UniProt Protein Name
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Adrenocortical dysplasia protein homolog
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| UniProt Synonym Protein Names
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POT1 and TIN2-interacting protein
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| Protein Family
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ACD11 homolog protein
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| UniProt Gene Name
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ACD
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[Similar Products]
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| UniProt Synonym Gene Names
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PIP1; PTOP; TINT1; TPP1
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[Similar Products]
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| UniProt Entry Name
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ACD_HUMAN
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| NCBI Summary for ACD
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This gene encodes a protein that is involved in telomere function. This protein is one of six core proteins in the telosome/shelterin telomeric complex, which functions to maintain telomere length and to protect telomere ends. Through its interaction with other components, this protein plays a key role in the assembly and stabilization of this complex, and it mediates the access of telomerase to the telomere. Multiple transcript variants encoding different isoforms have been found for this gene. This gene, which is also referred to as TPP1, is distinct from the unrelated TPP1 gene on chromosome 11, which encodes tripeptidyl-peptidase I. [provided by RefSeq, Jul 2008]
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| UniProt Comments for ACD
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ACD: Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Promotes binding of POT1 to single-stranded telomeric DNA. Modulates the inhibitory effects of POT1 on telomere elongation. The ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity. Plays a role in shelterin complex assembly. May play a role in organogenesis. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: nucleoplasm; chromosome, telomeric region; nuclear telomere cap complex; cytoplasm; nucleus
Molecular Function: protein binding; DNA binding
Biological Process: telomere assembly; intracellular protein transport; telomere capping; segmentation; positive regulation of telomerase activity; telomere maintenance; skeletal development; negative regulation of telomere maintenance via telomerase; urogenital system development; embryonic limb morphogenesis; protection from non-homologous end joining at telomere
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| Research Articles on ACD
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1. Shelterin protein TPP 1 interacts with hTERT and recruits hTERT onto the telomeres, suggesting that TPP 1 might be involved in regulation of telomere shortening.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with ACD elisa kit | Pathways associated with ACD elisa kit |
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| Diseases associated with ACD elisa kit | Organs/Tissues associated with ACD elisa kit |
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ELISA Kit 
