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ADAM12 elisa kit :: Human ADAM metallopeptidase domain 12 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704578
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $835 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ADAM metallopeptidase domain 12 (ADAM12), ELISA Kit

★Popular Item★
 Also Known As   

Human A Disintegrin And Metalloprotease 12, ADAM12 ELISA Kit

 Product Synonym Names    Human A Disintegrin And Metalloprotease 12; ADAM12 ELISA Kit; RP11-295J3.5; MCMP; MCMPMltna; MLTN; MLTNA; a disintegrin and metalloproteinase domain 12 (meltrin alpha) ; meltrin alpha; ADAM metallopeptidase domain 12
 Product Gene Name   

ADAM12 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704578 Testing Data    Testing Data PDF
 OMIM    602714
 3D Structure    ModBase 3D Structure for O43184
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ADAM12. No significant cross-reactivity or interference between Human ADAM12 and analogues was observed.
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 Samples    Serum, plasma, urine, cell culture supernates, tissue homogenates, Cell lysates
 Detection Range    31.25 pg/ml-2000 pg/ml
 Sensitivity    7.8 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Cell Adhesion
 Protein Biological Process 3    Cell adhesion
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADAM12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADAM12 purchase    MBS704578 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ADAM metallopeptidase domain 12 (ADAM12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml-2000 pg/ml in biological research samples containing ADAM12, with an estimated sensitivity of 7.8 pg/ml. The ELISA analytical biochemical technique of the MBS704578 kit is based on ADAM12 antibody-ADAM12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, urine, cell culture supernates, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADAM12 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADAM12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ADAM12 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADAM12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADAM12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ADAM12 elisa kit    ADAM12 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704578. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADAM12. It may not necessarily be applicable to this product.
 NCBI GI #    572882491
 NCBI GeneID    8038
 NCBI Accession #    NP_001275903.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001288974.1 [Other Products]
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 UniProt Primary Accession #    O43184 [Other Products]
 UniProt Secondary Accession #    O60470; Q5JRP0; Q5JRP1; Q6P9E3; Q6UWB0 [Other Products]
 UniProt Related Accession #    O43184 [Other Products]
 Molecular Weight    99,542 Da
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 NCBI Official Full Name    disintegrin and metalloproteinase domain-containing protein 12 isoform 4 preproprotein
 NCBI Official Synonym Full Names    ADAM metallopeptidase domain 12
 NCBI Official Symbol    ADAM12 [Similar Products]
 NCBI Official Synonym Symbols   
MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1
[Similar Products]
 NCBI Protein Information    disintegrin and metalloproteinase domain-containing protein 12; meltrin-alpha; metalloprotease-disintegrin 12 transmembrane
 UniProt Protein Name    Disintegrin and metalloproteinase domain-containing protein 12
 UniProt Synonym Protein Names   
Meltrin-alpha
 Protein Family    Disintegrin and metalloproteinase domain-containing protein
 UniProt Gene Name    ADAM12 [Similar Products]
 UniProt Synonym Gene Names    MLTN; UNQ346/PRO545; ADAM 12 [Similar Products]
 UniProt Entry Name    ADA12_HUMAN
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 NCBI Summary for ADAM12    This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]
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 UniProt Comments for ADAM12    Function: Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors

Cofactor: Binds 1 zinc ion per subunit.
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 Research Articles on ADAM12    1. ADAM12 is upregulated in the synovial tissue during synovitis on mRNA and protein level. and could have a role in knee osteoarthritis synovitis, especially in the occurrence of postinflammatory fibrosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADAM12 elisa kitPathways associated with ADAM12 elisa kit
 Reference Product  PubMed Publications
 ADAM17 elisa kit  >27 publications with ADAM12 and ADAM17
 ADAM10 elisa kit  >25 publications with ADAM12 and ADAM10
 EGF elisa kit  >24 publications with ADAM12 and EGF
 EGFR elisa kit  >13 publications with ADAM12 and EGFR
 DLL1 elisa kit  >2 publications with ADAM12 and DLL1
 IGFBP3 elisa kit  >2 publications with ADAM12 and IGFBP3
 ITGB1 elisa kit  >1 publications with ADAM12 and ITGB1
 SH3D19 elisa kit  >1 publications with ADAM12 and SH3D19
 PACSIN3 elisa kit  >1 publications with ADAM12 and PACSIN3
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By EGFR Pathway antibodies  Signaling By EGFR Pathway Diagram
 Signaling By EGFR In Cancer Pathway antibodies  Signaling By EGFR In Cancer Pathway Diagram
 Syndecan-4-mediated Signaling Events Pathway antibodies  Syndecan-4-mediated Signaling Events Pathway Diagram
Diseases associated with ADAM12 elisa kitOrgans/Tissues associated with ADAM12 elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >24 publications with ADAM12 and Necrosis
 Breast Neoplasms Antibodies  >21 publications with ADAM12 and Breast Neoplasms
 Hypertension Antibodies  >13 publications with ADAM12 and Hypertension
 Fibrosis Antibodies  >12 publications with ADAM12 and Fibrosis
 Lung Neoplasms Antibodies  >9 publications with ADAM12 and Lung Neoplasms
 Disease Models, Animal Antibodies  >9 publications with ADAM12 and Disease Models, Animal
 Muscular Diseases Antibodies  >8 publications with ADAM12 and Muscular Diseases
 Inflammation Antibodies  >8 publications with ADAM12 and Inflammation
 Cardiomegaly Antibodies  >7 publications with ADAM12 and Cardiomegaly
 Hypertrophy Antibodies  >7 publications with ADAM12 and Hypertrophy
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >43 publications with ADAM12 and Muscle
 Bone Antibodies  >19 publications with ADAM12 and Bone
 Embryonic Tissue Antibodies  >17 publications with ADAM12 and Embryonic Tissue
 Vascular Antibodies  >14 publications with ADAM12 and Vascular
 Brain Antibodies  >14 publications with ADAM12 and Brain
 Placenta Antibodies  >13 publications with ADAM12 and Placenta
 Heart Antibodies  >9 publications with ADAM12 and Heart
 Lung Antibodies  >8 publications with ADAM12 and Lung
 Connective Tissue Antibodies  >7 publications with ADAM12 and Connective Tissue
 Skin Antibodies  >5 publications with ADAM12 and Skin
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