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ADAMTS13 elisa kit :: Human ADAM metallopeptidase with thrombospondin type 1 motif, 13 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705447
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ADAM metallopeptidase with thrombospondin type 1 motif, 13 (ADAMTS13), ELISA Kit

★Popular Item★
 Also Known As   

Human von Willebrand Factor cleaving protease, ADAMTS-13/vWF-cp ELISA Kit

 Product Synonym Names    Human von Willebrand Factor cleaving protease; ADAMTS-13/vWF-cp ELISA Kit; C9orf8; DKFZp434C2322; FLJ42993; MGC118899; MGC118900; TTP; VWFCP; vWF-CP; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 13; vWF-cleaving protease; von Wil; ADAM metallopeptidase with thrombospondin type 1 motif; 13
 Product Gene Name   

ADAMTS13 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705447 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q769J6
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ADAMTS13. No significant cross-reactivity or interference between Human ADAMTS13 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, urine, cell culture supernates
 Detection Range    78 pg/ml-5000 pg/ml
 Sensitivity    19.5 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Blood Coagulation
 Protein Biological Process 3    Blood coagulation
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADAMTS13 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADAMTS13 purchase    MBS705447 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ADAM metallopeptidase with thrombospondin type 1 motif, 13 (ADAMTS13) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78 pg/ml-5000 pg/ml in biological research samples containing ADAMTS13, with an estimated sensitivity of 19.5 pg/ml. The ELISA analytical biochemical technique of the MBS705447 kit is based on ADAMTS13 antibody-ADAMTS13 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS13 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS13. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, urine, cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADAMTS13 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to vWF-cp. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for vWF-cp and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain vWF-cp, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of vWF-cp in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ADAMTS13 elisa kit    ADAMTS13 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705447. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADAMTS13. It may not necessarily be applicable to this product.
 NCBI GI #    47604978
 NCBI GeneID    279028
 NCBI Accession #    NP_001001322.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001001322.2 [Other Products]
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 UniProt Primary Accession #    Q769J6 [Other Products]
 UniProt Secondary Accession #    Q76LW1; A2ALB4 [Other Products]
 UniProt Related Accession #    Q769J6 [Other Products]
 Molecular Weight    155,357 Da
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 NCBI Official Full Name    A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 1
 NCBI Official Synonym Full Names    a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13
 NCBI Official Symbol    Adamts13 [Similar Products]
 NCBI Official Synonym Symbols   
Gm710; vWF-CP; ADAM-TS13; ADAMTS-13
[Similar Products]
 NCBI Protein Information    A disintegrin and metalloproteinase with thrombospondin motifs 13; vWF-cleaving protease; von Willebrand factor-cleaving protease; vWF-CP mRNA for von Willebrand factor-cleaving; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13
 UniProt Protein Name    A disintegrin and metalloproteinase with thrombospondin motifs 13
 UniProt Synonym Protein Names   
von Willebrand factor-cleaving protease; vWF-CP; vWF-cleaving protease
 Protein Family    A disintegrin and metalloproteinase with thrombospondin motifs
 UniProt Gene Name    Adamts13 [Similar Products]
 UniProt Synonym Gene Names    Gm710; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease [Similar Products]
 UniProt Entry Name    ATS13_MOUSE
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 UniProt Comments for ADAMTS13    Function: Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation.

Catalytic activity: Cleaves the vWF at the 842-Tyr-|-Met-843 in the A2 domain of the vWF subunit.

Cofactor: Binds 1 zinc ion per subunit

Enzyme regulation: Zinc and calcium ions cooperatively modulate enzyme activity. The cleavage of the pro-domain is not required for protease activity. Dependence on calcium for proteolytic activity is mediated by the high affinity site
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 Research Articles on ADAMTS13    1. Carboxyl terminus of ADAMTS13 directly inhibits platelet aggregation and ultra large von Willebrand factor string formation under flow in a free-thiol-dependent manner.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADAMTS13 elisa kitPathways associated with ADAMTS13 elisa kit
 Reference Product  PubMed Publications
 Vwf elisa kit  >597 publications with ADAMTS13 and Vwf
 Tsr2 elisa kit  >3 publications with ADAMTS13 and Tsr2
 Furin elisa kit  >3 publications with ADAMTS13 and Furin
 Pofut2 elisa kit  >1 publications with ADAMTS13 and Pofut2
 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 O-glycosylation Of TSR Domain-containing Proteins Pathway antibodies  O-glycosylation Of TSR Domain-containing Proteins Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
Diseases associated with ADAMTS13 elisa kitOrgans/Tissues associated with ADAMTS13 elisa kit
 Disease Name  Pubmed Publications
 Purpura, Thrombotic Thrombocytopenic Antibodies  >940 publications with ADAMTS13 and Purpura, Thrombotic Thrombocytopenic
 Thrombotic Microangiopathies Antibodies  >865 publications with ADAMTS13 and Thrombotic Microangiopathies
 Kidney Diseases Antibodies  >228 publications with ADAMTS13 and Kidney Diseases
 Purpura, Thrombocytopenic, Idiopathic Antibodies  >161 publications with ADAMTS13 and Purpura, Thrombocytopenic, Idiopathic
 Inflammation Antibodies  >75 publications with ADAMTS13 and Inflammation
 Fever Antibodies  >66 publications with ADAMTS13 and Fever
 Liver Diseases Antibodies  >44 publications with ADAMTS13 and Liver Diseases
 Disease Models, Animal Antibodies  >33 publications with ADAMTS13 and Disease Models, Animal
 Necrosis Antibodies  >23 publications with ADAMTS13 and Necrosis
 Liver Failure Antibodies  >21 publications with ADAMTS13 and Liver Failure
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >637 publications with ADAMTS13 and Blood
 Liver Antibodies  >70 publications with ADAMTS13 and Liver
 Kidney Antibodies  >68 publications with ADAMTS13 and Kidney
 Brain Antibodies  >33 publications with ADAMTS13 and Brain
 Connective Tissue Antibodies  >9 publications with ADAMTS13 and Connective Tissue
 Ovary Antibodies  >4 publications with ADAMTS13 and Ovary
 Intestine Antibodies  >3 publications with ADAMTS13 and Intestine
 Nerve Antibodies  >2 publications with ADAMTS13 and Nerve
 Placenta Antibodies  >1 publications with ADAMTS13 and Placenta
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