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ADAMTS5 elisa kit :: Human ADAM metallopeptidase with thrombospondin type 1 motif, 5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS911846
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5), ELISA Kit

★Popular Item★
 Also Known As   

Human A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAMTS5 ELISA Kit

 Product Synonym Names    Human A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA kit; ADAMTS11; ADMP-2; FLJ36738; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 5 (aggrecanase-2) ; aggrecanase-2; ADAM metallopeptidase with thrombospondin type 1 motif; 5
 Product Gene Name   

ADAMTS5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS911846 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q9TT92
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ADAMTS5. No significant cross-reactivity or interference between Human ADAMTS5 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    15.6 ng/ml-1000 ng/ml
 Sensitivity    3.9 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADAMTS5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADAMTS5 purchase    MBS911846 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 ng/ml-1000 ng/ml in biological research samples containing ADAMTS5, with an estimated sensitivity of 3.9 ng/ml. The ELISA analytical biochemical technique of the MBS911846 kit is based on ADAMTS5 antibody-ADAMTS5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADAMTS5 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ADAMTS5 elisa kit    ADAMTS5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS911846. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADAMTS5. It may not necessarily be applicable to this product.
 NCBI GI #    11131363
 NCBI GeneID    286805
 NCBI Accession #    Q9TT92.1 [Other Products]
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 UniProt Primary Accession #    Q9TT92 [Other Products]
 UniProt Related Accession #    Q9TT92 [Other Products]
 Molecular Weight    22,576 Da
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 NCBI Official Full Name    A disintegrin and metalloproteinase with thrombospondin motifs 5
 NCBI Official Synonym Full Names    ADAM metallopeptidase with thrombospondin type 1 motif, 5
 NCBI Official Symbol    ADAMTS5 [Similar Products]
 NCBI Protein Information    A disintegrin and metalloproteinase with thrombospondin motifs 5; ADMP-2; ADAM-TS5; ADAMTS-5; ADAM-TS 5; aggrecanase-2; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5
 UniProt Protein Name    A disintegrin and metalloproteinase with thrombospondin motifs 5
 UniProt Synonym Protein Names   
ADMP-2; Aggrecanase-2
 Protein Family    A disintegrin and metalloproteinase with thrombospondin motifs
 UniProt Gene Name    ADAMTS5 [Similar Products]
 UniProt Synonym Gene Names    ADAM-TS 5; ADAM-TS5; ADAMTS-5 [Similar Products]
 UniProt Entry Name    ATS5_BOVIN
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 UniProt Comments for ADAMTS5    Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.

Catalytic activity: Cleaves aggrecan at the 392-Glu-|-Ala-393 site.

Cofactor: Binds 1 zinc ion per subunit
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 Research Articles on ADAMTS5    1. The delayed activation of proMMPs and the relatively low cleavage efficiency of MMPs compared to ADAMTS5 (aggrecanase) explains the minor contribution of the MMP enzymes to aggrecan catabolism in vivo.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADAMTS5 elisa kitPathways associated with ADAMTS5 elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with ADAMTS5 and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with ADAMTS5 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Ovary Antibodies  >2 publications with ADAMTS5 and Ovary
 Uterus Antibodies  >1 publications with ADAMTS5 and Uterus
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