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ADAMTS8 elisa kit :: Human ADAM metallopeptidase with thrombospondin type 1 motif, 8 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS945705
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ADAM metallopeptidase with thrombospondin type 1 motif, 8 (ADAMTS8), ELISA Kit

 Also Known As   

Human A disintegrin and metalloproteinase with thrombospondin motifs 8, ADAMTS8 ELISA Kit

 Product Synonym Names    Human A disintegrin and metalloproteinase with thrombospondin motifs 8 (ADAMTS8) ELISA kit; ADAM-TS8; FLJ41712; METH2; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 8; ADAM metallopeptidase with thrombospondin type 1 motif; 8
 Product Gene Name   

ADAMTS8 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS945705 Testing Data    Testing Data PDF
 OMIM    605175
 3D Structure    ModBase 3D Structure for Q9UP79
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human ADAMTS8. No significant cross-reactivity or interference between human ADAMTS8 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    1.56 ng/ml -100 ng/ml.
 Sensitivity    The minimum detectable dose of human ADAMTS8 is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of ADAMTS8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADAMTS8 purchase    MBS945705 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ADAM metallopeptidase with thrombospondin type 1 motif, 8 (ADAMTS8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56 ng/ml -100 ng/ml in biological research samples containing ADAMTS8, with an estimated sensitivity of The minimum detectable dose of human ADAMTS8 is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS945705 kit is based on ADAMTS8 antibody-ADAMTS8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ADAMTS8 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ADAMTS8 elisa kit    ADAMTS8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS945705
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NCBI/Uniprot data below describe general gene information for ADAMTS8. It may not necessarily be applicable to this product.
 NCBI GI #    153792351
 NCBI GeneID    11095
 NCBI Accession #    NP_008968.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_007037.4 [Other Products]
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 UniProt Primary Accession #    Q9UP79 [Other Products]
 UniProt Secondary Accession #    Q9NZS0 [Other Products]
 UniProt Related Accession #    Q9UP79 [Other Products]
 Molecular Weight    96,460 Da
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 NCBI Official Full Name    A disintegrin and metalloproteinase with thrombospondin motifs 8 preproprotein
 NCBI Official Synonym Full Names    ADAM metallopeptidase with thrombospondin type 1 motif, 8
 NCBI Official Symbol    ADAMTS8 [Similar Products]
 NCBI Official Synonym Symbols   
METH2; ADAM-TS8
[Similar Products]
 NCBI Protein Information    A disintegrin and metalloproteinase with thrombospondin motifs 8; METH-2; METH-8; ADAMTS-8; ADAM-TS 8; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 8
 UniProt Protein Name    A disintegrin and metalloproteinase with thrombospondin motifs 8
 UniProt Synonym Protein Names   
METH-2; METH-8
 Protein Family    A disintegrin and metalloproteinase with thrombospondin motifs
 UniProt Gene Name    ADAMTS8 [Similar Products]
 UniProt Synonym Gene Names    METH2; ADAM-TS 8; ADAM-TS8; ADAMTS-8 [Similar Products]
 UniProt Entry Name    ATS8_HUMAN
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 NCBI Summary for ADAMTS8    This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs, and disrupts angiogenesis in vivo. A number of disorders have been mapped in the vicinity of this gene, most notably lung neoplasms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ADAMTS8    ADAMTS8: Has anti-angiogenic properties.

Protein type: Cell cycle regulation; Motility/polarity/chemotaxis; EC 3.4.24.-; Protease; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q25

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: heparin binding; integrin binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; low affinity phosphate transmembrane transporter activity

Biological Process: negative regulation of cell proliferation; proteolysis
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 Research Articles on ADAMTS8    1. ADAMTS 8, CCL23, and TNFSF15 are implicated in anti-angiogenic activities
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with ADAMTS8 elisa kitOrgans/Tissues associated with ADAMTS8 elisa kit
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >3 publications with ADAMTS8 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >2 publications with ADAMTS8 and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >2 publications with ADAMTS8 and Nervous System Diseases
 Adenocarcinoma Antibodies  >2 publications with ADAMTS8 and Adenocarcinoma
 Brain Diseases Antibodies  >2 publications with ADAMTS8 and Brain Diseases
 Atherosclerosis Antibodies  >1 publications with ADAMTS8 and Atherosclerosis
 Brain Ischemia Antibodies  >1 publications with ADAMTS8 and Brain Ischemia
 Myocardial Infarction Antibodies  >1 publications with ADAMTS8 and Myocardial Infarction
 Liver Diseases Antibodies  >1 publications with ADAMTS8 and Liver Diseases
 Hypertension Antibodies  >1 publications with ADAMTS8 and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >2 publications with ADAMTS8 and Heart
 Brain Antibodies  >2 publications with ADAMTS8 and Brain
 Lung Antibodies  >2 publications with ADAMTS8 and Lung
 Connective Tissue Antibodies  >1 publications with ADAMTS8 and Connective Tissue
 Pancreas Antibodies  >1 publications with ADAMTS8 and Pancreas
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