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AEBP1 elisa kit :: Human AE Binding Protein 1 (AEBP1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2022177 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AE Binding Protein 1 (AEBP1), ELISA Kit

★Popular Item★
 Also Known As   

AE Binding Protein 1 (AEBP1) ELISA Kit

 Product Synonym Names    ACLP; Aortic Carboxypeptidase-Like Protein; Adipocyte Enhancer Binding Protein 1
 Product Gene Name   

AEBP1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    602981
 3D Structure    ModBase 3D Structure for Q8IUX7
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of AE Binding Protein 1 (AEBP1).
No significant cross-reactivity or interference between AE Binding Protein 1 (AEBP1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.78-50ng/mL
 Sensitivity    < 0.32ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level AE Binding Protein 1 (AEBP1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level AE Binding Protein 1 (AEBP1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AEBP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AEBP1 purchase    MBS2022177 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AE Binding Protein 1 (AEBP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78-50ng/mL in biological research samples containing AEBP1, with an estimated sensitivity of < 0.32ng/mL. The ELISA analytical biochemical technique of the MBS2022177 kit is based on AEBP1 antibody-AEBP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AEBP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AEBP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for AEBP1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AE Binding Protein 1 (AEBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to AE Binding Protein 1 (AEBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AE Binding Protein 1 (AEBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AE Binding Protein 1 (AEBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for AEBP1 elisa kit    Metabolic pathway
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 Typical Testing Data/Standard Curve (for reference only) of AEBP1 elisa kit    AEBP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2022177. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AEBP1. It may not necessarily be applicable to this product.
 NCBI GI #    119581523
 NCBI GeneID    165
 NCBI Accession #    EAW61119.1 [Other Products]
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 UniProt Primary Accession #    Q8IUX7 [Other Products]
 UniProt Secondary Accession #    Q14113; Q59ER7; Q6ZSC7; Q7KZ79 [Other Products]
 UniProt Related Accession #    Q8IUX7 [Other Products]
 Molecular Weight    82,183 Da
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 NCBI Official Full Name    AE binding protein 1
 NCBI Official Synonym Full Names    AE binding protein 1
 NCBI Official Symbol    AEBP1 [Similar Products]
 NCBI Official Synonym Symbols   
ACLP
[Similar Products]
 NCBI Protein Information    adipocyte enhancer-binding protein 1
 UniProt Protein Name    Adipocyte enhancer-binding protein 1
 UniProt Synonym Protein Names   
Aortic carboxypeptidase-like protein
 Protein Family    Adipocyte enhancer-binding protein
 UniProt Gene Name    AEBP1 [Similar Products]
 UniProt Synonym Gene Names    ACLP; AE-binding protein 1 [Similar Products]
 UniProt Entry Name    AEBP1_HUMAN
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 NCBI Summary for AEBP1    This gene encodes a member of carboxypeptidase A protein family. The encoded protein may function as a transcriptional repressor and play a role in adipogenesis and smooth muscle cell differentiation. Studies in mice suggest that this gene functions in wound healing and abdominal wall development. Overexpression of this gene is associated with glioblastoma. [provided by RefSeq, May 2013]
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 UniProt Comments for AEBP1    AEBP1: May positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation. May also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I- kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness. Can act as a transcriptional repressor. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: extracellular matrix; extracellular space; cytoplasm; nucleus

Molecular Function: calmodulin binding; carboxypeptidase activity; zinc ion binding; transcription factor activity; metallocarboxypeptidase activity; transcription corepressor activity

Biological Process: muscle development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; proteolysis; skeletal development
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 Research Articles on AEBP1    1. ACLP stimulates the fibroblast-to-myofibroblast transition by promoting SMA expression via TGFbeta signaling and promoting collagen expression through a TGFbeta receptor-independent pathway.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AEBP1 elisa kitDiseases associated with AEBP1 elisa kit
 Reference Product  PubMed Publications
 PTEN elisa kit  >4 publications with AEBP1 and PTEN
 TAGLN elisa kit  >1 publications with AEBP1 and TAGLN
 THBS2 elisa kit  >1 publications with AEBP1 and THBS2
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >9 publications with AEBP1 and Inflammation
 Cardiovascular Diseases Antibodies  >4 publications with AEBP1 and Cardiovascular Diseases
 Necrosis Antibodies  >3 publications with AEBP1 and Necrosis
 Hyperplasia Antibodies  >2 publications with AEBP1 and Hyperplasia
 Prostatic Neoplasms Antibodies  >1 publications with AEBP1 and Prostatic Neoplasms
 Genital Diseases, Male Antibodies  >1 publications with AEBP1 and Genital Diseases, Male
 Dyslipidemias Antibodies  >1 publications with AEBP1 and Dyslipidemias
 Weight Gain Antibodies  >1 publications with AEBP1 and Weight Gain
 HIV Infections Antibodies  >1 publications with AEBP1 and HIV Infections
 Ovarian Diseases Antibodies  >1 publications with AEBP1 and Ovarian Diseases
Organs/Tissues associated with AEBP1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >11 publications with AEBP1 and Muscle
 Vascular Antibodies  >10 publications with AEBP1 and Vascular
 Connective Tissue Antibodies  >8 publications with AEBP1 and Connective Tissue
 Adipose Tissue Antibodies  >8 publications with AEBP1 and Adipose Tissue
 Bone Antibodies  >8 publications with AEBP1 and Bone
 Embryonic Tissue Antibodies  >6 publications with AEBP1 and Embryonic Tissue
 Lung Antibodies  >4 publications with AEBP1 and Lung
 Blood Antibodies  >4 publications with AEBP1 and Blood
 Mammary Gland Antibodies  >3 publications with AEBP1 and Mammary Gland
 Heart Antibodies  >3 publications with AEBP1 and Heart
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